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2 protocols using desformylflustrabromine hydrochloride

1

Radioligand Binding Assay Reagents

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[7,8-3H]Dopamine (21.2 Ci/mmol (0.784 TBq/mmol)) and [1-7,8-3H]noradrenaline (12.1 Ci/mmol (0.448 TBq/mmol)) were purchased from Perkin Elmer, Monza, Italy. Nicotine hydrogen tartrate salt, choline, 4-aminopyridine, 6-nitroquipazine, desipramine, beta-amyloid (40-1), horseradish peroxidase-conjugated anti-mouse secondary antibody, Ponceau Red were from Sigma (Sigma-Aldrich, St Louis, MO, USA); Monoclonal antibody 6E10 was from Covance ImmunoTechnologies, Dedham, MA; 5-iodo-A-85380, dihydro-β-erythroidine, α-conotoxin MII, oxotremorine sesquifumarate, desformylflustrabromine hydrochloride, galantamine hydrobromide, SR 165845, beta-amyloid (1-40) were from Tocris (Tocris Bioscience, Bristol, UK).
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2

Radioligands and Pharmacological Tools for Neuroscience Research

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[7,8-3H] Dopamine (21.2 Ci/mmol (0.784 TBq/mmol)) and [1-7,8-3H] Noradrenaline (12.1 Ci/mmol (0.448 TBq/mmol)) were purchased from Perkin Elmer, Monza, Italy. Nicotine hydrogen tartrate salt was from Sigma (Sigma-Aldrich, St. Louis, MO, USA). 5-iodo-A-85380, α-conotoxin MII, oxotremorine, desformylflustrabromine hydrochloride, beta-amyloid (1–40) were from Tocris (Tocris Bioscience, Bristol, UK). AnnexinV conjugate PE was from Miltenyi Biotec GmbH (order n° 130-118-363) and CalceinAM was from Biotium (catalog n° 30026). KLVFF (segment Aβ 16-20) and VFLKF peptide were purchased from Aurogene s.r.l. (Rome, Italy)
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