Animals were deeply anesthetized with a mixture of Xylazine and Ketamine (1:8), washed with 1X saline for 1 min and then perfused with 4% paraformaldehyde (PFA) for 15–20 min. Brains were quickly dissected out and immediately stored in 4% PFA for 24 h at 4°C, and then transferred to 30% sucrose at 4°C for 48 h. Brains were cut using a cryostat at 4°C into 20-micron-thick coronal sections and stored at -20°C. Immunohistochemistry was performed on 20 μm-thick sections. Astrocytes were probed (1:200) with monoclonal anti-GFAP antibody (Millipore Corporation, USA), and microglia were probed (1:200) with IBA-1 polyclonal antibody (Wako, USA). Dendritic cells and/or microglia were probed (1:200) with CD11b polyclonal antibodies (Thermo Fisher, USA). Nuclear staining with 4′,6-Diamidino-2-Phenylindole (DAPI) was performed according to manufacturer’s protocols (Life Technologies, USA).
Cd11b polyclonal antibodies
Manufactured by Thermo Fisher Scientific
Sourced in United States
CD11b polyclonal antibodies are laboratory reagents used in various immunological and cell biology applications. They are designed to detect and bind to the CD11b protein, which is expressed on the surface of certain immune cells. These antibodies can be used for the identification, quantification, and isolation of cell populations of interest.
Automatically generated - may contain errors
2 protocols using cd11b polyclonal antibodies
1
Immunohistochemical Analysis of Glial Cells
2
Immunohistochemical Analysis of Glial Cells
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Animals were deeply anesthetized with a mixture of Xylazine and Ketamine (1:8), washed with 1X saline for 1 min and then perfused with 4% paraformaldehyde (PFA) for 15–20 min. Brains were quickly dissected out and immediately stored in 4% PFA for 24 h at 4°C, and then transferred to 30% sucrose at 4°C for 48 h. Brains were cut using a cryostat at 4°C into 20-micron-thick coronal sections and stored at -20°C. Immunohistochemistry was performed on 20 μm-thick sections. Astrocytes were probed (1:200) with monoclonal anti-GFAP antibody (Millipore Corporation, USA), and microglia were probed (1:200) with IBA-1 polyclonal antibody (Wako, USA). Dendritic cells and/or microglia were probed (1:200) with CD11b polyclonal antibodies (Thermo Fisher, USA). Nuclear staining with 4′,6-Diamidino-2-Phenylindole (DAPI) was performed according to manufacturer’s protocols (Life Technologies, USA).
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