Symphony automated platform

Manufactured by Roche

The Symphony automated platform is a laboratory equipment designed for high-throughput sample processing and analysis. It provides automated sample handling, liquid handling, and detection capabilities to streamline various laboratory workflows. The Symphony platform is intended to enhance efficiency and productivity in research and diagnostic settings, but a detailed description of its intended use is not available.

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Lab products found in correlation

Isotype control, by Abcam (1 mentions) Intellipath autostainer, by Biocare Medical (1 mentions) Aperio software, by Leica Biosystems (1 mentions) Intellipath, by Biocare Medical (1 mentions) Clone sp7, by Abcam (1 mentions)

2 protocols using symphony automated platform

Immunohistochemical Analysis of Salivary Tissue

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Formalin fixed, paraffin embedded tissue was stained with H&E using the Symphony automated platform (Ventana). Immunohistochemistry (IHC) was performed on salivary tissue with an IntelliPATH autostainer (BioCare Medical) using an Opn antibody (0.07 μg/mL) (LifeSpan Biosciences). An isotype control (Abcam) was used at the same concentration.

Husain-Krautter S., Kramer J.M., Li W., Guo B, & Rothstein T.L. (2015). The Osteopontin Transgenic Mouse is a New Model for Sjögren’s Syndrome. Clinical immunology (Orlando, Fla.), 157(1), 30-42.

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Histological Analysis of Salivary Tissue

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Salivary tissue was fixed in formalin, paraffin embedded, and processed using a Tissue Tek VIP (Sakura). H&E staining was performed using a Symphony automated platform (Ventana). Slides were scanned using Aperio software (Leica Biosystems) and lymphocytic infiltration was quantified using ImageJ. The presence of periductal FLS within submandibular salivary tissue was considered indicative of SS disease. Periductal FLS was quantified by measuring the percent of SMG tissue occupied by lymphocytes [30 (link)]. Immunohistochemical staining (IHC) was performed on salivary and splenic tissue with an IntelliPATH autostainer (BioCare Medical) using antibodies directed against B220 (2 μg/mL, clone RA3-6B2, Abcam) and CD3 (1:100 dilution, clone SP7, Abcam). Spectral phasor analysis was carried out using a custom ImageJ plugin (available from http://www.spechron.com). The phasor approach has previously been implemented to analyse lifetime [39 (link), 40 (link)] and spectral images [41 (link), 42 (link)]. A detailed description of this analysis is provided in the appendix.

Sharma A., Kiripolsky J., Klimatcheva E., Howell A., Fereidouni F., Levenson R., Rothstein T.L, & Kramer J.M. (2016). Early BAFF receptor blockade mitigates murine Sjögren's syndrome: Concomitant targeting of CXCL13 and the BAFF receptor prevents salivary hypofunction. Clinical immunology (Orlando, Fla.), 164, 85-94.

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