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4 protocols using potassium permanganate

1

Enzymatic Lipid Modification Protocol

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Four genes (Photobacterium lipolyticum M37 lipase [25 (link)-28 (link, link, link)], Proteus vulgaris K80 lipase [29 (link)], Bacillus stearothermophilus L1 lipase [30 (link)], Staphylococcus haemolyticus L62 lipase [31 (link)]) isolated previously by our laboratory were used in this study. Candida antarctica lipase B (CalB) enzyme was purchased from Novozyme. Alcalase (Bacillus licheniformis protease), diethyl malate, p-nitrophenyl caprylate, silica gel (Davisil Grade 635, pore size 60Å, 60-100 mesh), and 1-butanol were purchased from Sigma-Aldrich. Acetic acid was purchased from Kanto. Potassium permanganate and formic acid were purchased from Daejung. Sodium hydroxide and potassium carbonate were purchased from Jusei. Acetonitrile was purchased from Duksan.
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2

Graphite-based Material Synthesis

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Graphite (Sigma-Aldrich, 325 mesh), sulfuric acid (H2SO4, 95%, Daejung), phosphoric acid (H3PO4, 85%, Daejung), potassium permanganate (KMnO4, 99.0%, Daejung), hydrogen peroxide (H2O2, 30%, Daejung), hydrochloric acid (HCl, 35%, Daejung), and potassium hydroxide (KOH, flake, Daejung), were used for the synthesis process. These chemicals were used as received without further purification. Deionized water was used in all experiments.
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3

Synthesis of Inorganic Compounds

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Zinc sulfate heptahydrate (ZnSO4·7H2O,
99%, MW 287.56 g mol–1), potassium sulfate (K2SO4, 99%, MW 174.24 g mol–1),
sodium chloride (NaCl, 99%, MW 58.44 g mol–1), anhydrous
magnesium sulfate (MgSO4, 99%, MW 120.37 g mol–1), and ethyl alcohol (C2H5OH, 94%) were purchased
from Duksan Pure Chemicals (South Korea). N,N-Dimethylformamide (C3H7NO, 99.5%,
MW 73.1 g mol–1), oxalic acid dihydrate (H2C2O4·2H2O, 99.5–100.2%,
MW 126.07 g mol–1), 5 N sodium hydroxide standard
solution, potassium permanganate (KMnO4, 99.3%, MW 158.03
g mol–1), and manganese(II) sulfate pentahydrate
(MnSO4·5H2O, 98%, MW 241.08 g mol–1) were purchased from Daejung Chemicals & Metals (South Korea).
Calcium sulfate dihydrate (CaSO4·2H2O,
95%, MW 172.17 g mol–1) was purchased from Oriental
Chemical Industries (South Korea). Strontium nitrate (Sr(NO3)2, 99%, MW 211.63 g mol–1) was purchased
from Sigma-Aldrich (USA) and used to prepare simulant solutions. All
solutions were prepared with deionized (D.I.) water (EXL 5 A16, EXL
Service, USA).
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4

Graphite-based Chemical Synthesis Protocol

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Graphite was purchased from Kropfmühl AG (Herzenberg, Germany). Potassium permanganate, sodium nitrate, sulfuric acid, hydrogen peroxide (30%), pyrogallol, and hydrochloric acid (35–37%) were purchased from Daejung Chemicals (Siheung, Korea). Polyethylene imine (MW: 7 50 000) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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