Edta containing vacutainers

Manufactured by BD

Sourced in United Kingdom

EDTA-containing vacutainers are laboratory blood collection tubes that are pre-coated with the anticoagulant ethylenediaminetetraacetic acid (EDTA). The primary function of EDTA-containing vacutainers is to prevent blood samples from clotting, allowing for accurate analysis and testing.

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Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Sepmate pbmc isolation tube, by STEMCELL (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Histopaque, by Merck Group (1 mentions) Human magnetic cytokine panel, by Thermo Fisher Scientific (1 mentions) Sw41 rotor, by Beckman Coulter (1 mentions) Accuri c6, by BD (1 mentions) Attune nxt, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Collagenase type 2, by Worthington (1 mentions) Cellrox deep red reagent, by Thermo Fisher Scientific (1 mentions) Dnase, by Merck Group (1 mentions) Complete dmem medium, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

BD Vacutainer (1960 citations) EDTA vacutainers (182 citations) EDTA-containing vacutainer tubes (11 citations) EDTA containing vacutainer sets (2 citations)

9 protocols using edta containing vacutainers

Isolation and Cryopreservation of PBMCs

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Before steroid treatment, venous blood (100 mL) was drawn into EDTA-containing vacutainers (Becton Dickinson) and mixed (1:1) with phosphate-buffered saline. Blood-buffer mix (35 mL) was layered on 15 mL of Histopaque (1.077g/mL; Sigma-Aldrich) in 50 mL SepMate PBMC Isolation Tubes (STEMCELL Technologies), centrifuged (1,200g, 10 minutes, RT), supernatants transferred to 50 mL tubes, and further centrifuged (400g, 10 minutes, RT). Pellets were resuspended (10⁷ cells/mL) in freezing media [10% FBS; Scientifix] and 10% dimethyl sulfoxide (Sigma-Aldrich)] in RPMI 1640 supplemented with l-glutamine (ThermoFisher Scientific). Purification of peripheral blood mononuclear cells (PBMC) suspensions (1 mL) were added to cryotubes for controlled freezing at −80°C and then stored in liquid nitrogen.

Binder M.D., Nwoke E.C., Morwitch E., Dwyer C., Li V., Xavier A., Lea R.A., Lechner-Scott J., Taylor B.V., Ponsonby A.L, & Kilpatrick T.J. (2023). HLA-DRB1*15:01 and the MERTK Gene Interact to Selectively Influence the Profile of MERTK-Expressing Monocytes in Both Health and MS. Neurology® Neuroimmunology & Neuroinflammation, 11(2), e200190.

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Plasma Preparation for Analyses

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Venous blood samples were collected in EDTA containing vacutainers (Becton Dickinson & Company, Mount Wellington, New Zealand), and plasma was removed after centrifugation at 2,000 x g for 15 min at 4°C and frozen at −80°C prior to analyses.

Milan A.M., Samuelsson L.M., Shrestha A., Sharma P., Day L, & Cameron-Smith D. (2020). Circulating Branched Chain Amino Acid Concentrations Are Higher in Dairy-Avoiding Females Following an Equal Volume of Sheep Milk Relative to Cow Milk: A Randomized Controlled Trial. Frontiers in Nutrition, 7, 553674.

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Exercise Performance and Blood Markers

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The main experimental trial was conducted following a rest period of 7 days. Participants were instructed to abstain from exercise, alcohol, and caffeine for the 24‐h period prior to testing. Participants arrived at 0900 following an overnight 12‐h fast, during which they were allowed to consume water ad libitum. Height and weight were measured using standard procedures, and 22.5 mL of blood was then collected into EDTA containing vacutainers (Becton Dickinson, Oxford, UK) from a vein in the antecubital fossa of the forearm. The main protocol required participants to run at 60% of vV˙O2max for 120‐min, interspersed with sprints at 90% of vV˙O2max for the last 30‐sec of every 10‐min. Immediately upon completion of the run, a further blood sample was taken using the same procedure as previously described. Pre‐ and postrun blood samples were centrifuged at 1700 g for 10‐min at 4°C in order for plasma to be separated. Samples were immediately stored at −80°C for later analysis.

Horsburgh S., Todryk S., Toms C., Moran C.N, & Ansley L. (2015). Exercise‐conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiological Reports, 3(12), e12621.

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Cytokine Profiling of Emergency Samples

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Participant blood samples were collected within 24 hours of emergency department presentation in EDTA-containing vacutainers (BD Biosciences, San Jose, CA), transported on ice, spun down at 2500g for 10 min at 4°C, and stored at −80°C until further analysis. Cell-free plasma was analyzed using a human magnetic cytokine panel providing simultaneous measurement of 35 cytokines (Thermo Fisher Scientific, Waltham, MA). The assay was performed according to the manufacturer’s instructions with each subject sample performed in duplicate and then analyzed on a Luminex FLEXMAP 3D instrument.

Kim J.G., Zhang A., Rauseo A.M., Goss C.W., Mudd P.A., O'Halloran J.A, & Wang L. (2023). The salivary and nasopharyngeal microbiomes are associated with SARS‐CoV‐2 infection and disease severity. Journal of Medical Virology, 95(2), e28445.

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Measuring Plasma Cytokines and Biomarkers

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MT-DNA and other cytokines were measured in cell-free plasma of subjects within the first 24 hours of emergency department presentation. Blood samples were collected in EDTA-containing vacutainers (BD Biosciences) and subjected to 2 rounds of centrifugation to generate platelet poor plasma, first at 2500g for 20 minutes to generate plasma. The plasma was removed from vacutainers and centrifuged at 13,000g in sterile nuclease-free eppendorf tubes (Thermo Fisher Scientific) for 10 minutes to remove platelets. These platelet-poor specimens were then immediately stored at −80°C until further analysis. Concurrently measured clinical markers (i.e., CRP, ferritin, lactate dehydrogenase, D-dimer) were obtained from the electronic medical record.

Scozzi D., Cano M., Ma L., Zhou D., Zhu J.H., O’Halloran J.A., Goss C., Rauseo A.M., Liu Z., Sahu S.K., Peritore V., Rocco M., Ricci A., Amodeo R., Aimati L., Ibrahim M., Hachem R., Kreisel D., Mudd P.A., Kulkarni H.S, & Gelman A.E. (2021). Circulating mitochondrial DNA is an early indicator of severe illness and mortality from COVID-19. JCI Insight, 6(4), e143299.

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Isolation of Plasma-Derived Small Extracellular Vesicles

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Venous blood samples were drawn from rats and patients into EDTA-containing vacutainers (BD biosciences, USA). The plasma was obtained by centrifuging at 1600 × g for 20 minutes at 4°C and subsequently stored at -80°C for further experiments. Plasma sEV were purified using differential ultracentrifugation according to the guideline [16 ]. Briefly, plasma was centrifuged at 10,000 × g for 30 minutes at 4°C to remove cells and debris, then twice at 100,000 × g for 1 hour at 4°C with a SW-41 rotor (Beckman Coulter, USA). The pellets were washed with phosphate buffered saline (PBS) and centrifuged again at 100,000 × g for 1 hour at 4°C to purify the sEV. The isolated plasma sEV were collected and resuspended in 1x PBS for subsequent analysis.

Gao X.F., Wang Z.M., Chen A.Q., Wang F., Luo S., Gu Y., Kong X.Q., Zuo G.F., Jiang X.M., Ding G.W., Chen Y., Ge Z., Zhang J.J, & Chen S.L. (2021). Plasma Small Extracellular Vesicle-Carried miRNA-501-5p Promotes Vascular Smooth Muscle Cell Phenotypic Modulation-Mediated In-Stent Restenosis. Oxidative Medicine and Cellular Longevity, 2021, 6644970.

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Immune Cell Profiling from Biological Samples

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Blood was collected from the jugular vein using EDTA-containing vacutainers (BD Biosciences, San Jose, CA). BALF was centrifuged to analyze the cellular composition of the cell pellet. Lung, spleen, and TBLNs were dispersed into single cells as previously described (Artiaga et al., 2014 (link)). Leukocytes obtained from homogenized or digested tissues were treated with an ammonium chloride-based lysis buffer to eliminate residual red blood cells. Immune cell populations were characterized using either a BD Accuri C6, BD LSRFortessa (BD Bioscience) or Attune NxT (Thermofisher, Grand Island, NY) flow cytometer after cells were blocked with polyclonal rat IgG antibody and stained as previously described (Artiaga et al., 2016a (link)). Antibodies and tetramer reagents used to characterize iNKT cells, αβ T cells, γδ T cells, and NK cells are described in Supplemental Table 1. Data were analyzed using FlowJo software v9 (Treestar, Palo Alto, CA).

Gu W., Madrid D.M., Yang G., Artiaga B.L., Loeb J.C., Castleman W.L., Richt J.A., Lednicky J.A, & Driver J.P. (2020). Unaltered influenza disease outcomes in swine prophylactically treated with α-galactosylceramide. Developmental and comparative immunology, 114, 103843.

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Comprehensive Lung Immune Profiling

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BAL was collected from the left bronchus and cells and pelleted at 500 x g for 20 mins to produce BALf. Blood was collected in EDTA-containing vacutainers (BD Sciences). Lung cellular fractionation was conducted by tissue mincing with forceps and digestion with RPMI with Type II collagenase (0.5 mg/ml) (Worthington Biochemical) and 5 U/mL DNAse (Sigma) and transferred to a 37°C incubator in mild agitation for 1 hour. Cell pellets were resuspended in FACS buffer (PBS with 2% FBS and 0.4% EDTA) and stained with CD45 (eBioscience, 30-F11), CD11c (BD Pharmigen, HL3), Ly6C (Biolegend, HK1.4), Ly6G (Biolegend, 1A8), Gr1 (Biolegend, RB6–8C5) and CD11b (eBioscince, M1/70). For ROS analysis BAL pellets were incubated with CellROX deep red reagent (Thermo Fisher) for 30 mins at 37 ^oC in DMEM complete medium (Gibco). Detection of CXCL1, CXCL2, and CXCL5 from BALf was performed using ProcartaPlex Mouse Cytokine & Chemokine Immunoassays (eBioscience) in accordance with the manufacturer`s instructions.

Scozzi D., Ibrahim M., Liao F., Lin X., Hsiao H.M., Hachem R., Tague L., Ricci A., Kulkarni H.S., Huang H.J., Sugimoto S., Krupnick A.S., Kreisel D, & Gelman A.E. (2019). Mitochondrial damage associated molecular patterns released by lung transplants are associated with primary graft dysfunction. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(5), 1464-1477.

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Sleep Disorder Screening in Healthy Volunteers

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Blood samples were obtained from 15 (7 males/ 8 females) healthy non-smoker volunteers with a mean age of 26.6 ± 3 years and BMI of 23.7 ± 4.5 Kg/m². Sleep studies were performed on all subjects using the WatchPAT-100 device (21 (link)), to rule out occult SDB. All subjects had less than 5 oxygen desaturation breathing events per hour (ODI) which is considered a normal value. The protocol was approved by the local Human Rights Committee of RAMBAM Medical Center according to the Declaration of Helsinki, and all participants signed an informed consent form. Some of the subjects were tested two to six times. All blood samples were withdrawn in the morning hours, collected into EDTA containing vacutainers (BD Plymouth, UK) and kept on ice until use.

Avezov K., Aizenbud D, & Lavie L. (2018). Intermittent Hypoxia Induced Formation of “Endothelial Cell-Colony Forming Units (EC-CFUs)” Is Affected by ROS and Oxidative Stress. Frontiers in Neurology, 9, 447.

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