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2 protocols using oasis mcx 3 cc 60 mg cartridges

1

Beef Hydrocolloid and AGE Profiling

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Beef was obtained from a local market in Changsha, China. Hydrocolloids including alginic acid, chitosan, carrageenan, xanthan gum, konjac glucomannan (KGM), pectin and gellan gum were purchased from Aladdin (Shanghai, China). Chemical standards of HAs: PhIP, IQ, MeIQ, MeIQx, 4,8-DiMeIQx, IQx, Harmane and NorHarmane were supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Chemical standards of AGEs, Nε-(carboxymethyl)lysine (CML), d4-CML, Nε-(carboxyethyl)lysine (CEL) and d4-CEL, were obtained by Santa Cruz Biotechnology Co. (Paso Robles, CA, USA). The standards of lysine, phenylalanine, creatinine, glucose, threonine, tryptophan, phenylacetaldehyde, 2,5-dimethylpyrazine, glyoxal, methylglyoxal, O-phenylendiamine, 2,3-hexanedione and nonafluoropentanoic acid (NFPA) were purchased from J&K Scientific Co, Ltd. (Beijing, China). Mass spectral grade methanol and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany). Oasis MCX (3 cc/60 mg) cartridges were purchased from Waters (Milford, MA, USA). The chemicals were all analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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2

Beef Hydrocolloid and AGE Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Beef was obtained from a local market in Changsha, China. Hydrocolloids including alginic acid, chitosan, carrageenan, xanthan gum, konjac glucomannan (KGM), pectin and gellan gum were purchased from Aladdin (Shanghai, China). Chemical standards of HAs: PhIP, IQ, MeIQ, MeIQx, 4,8-DiMeIQx, IQx, Harmane and NorHarmane were supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Chemical standards of AGEs, Nε-(carboxymethyl)lysine (CML), d4-CML, Nε-(carboxyethyl)lysine (CEL) and d4-CEL, were obtained by Santa Cruz Biotechnology Co. (Paso Robles, CA, USA). The standards of lysine, phenylalanine, creatinine, glucose, threonine, tryptophan, phenylacetaldehyde, 2,5-dimethylpyrazine, glyoxal, methylglyoxal, O-phenylendiamine, 2,3-hexanedione and nonafluoropentanoic acid (NFPA) were purchased from J&K Scientific Co, Ltd. (Beijing, China). Mass spectral grade methanol and acetonitrile were purchased from Merck KGaA (Darmstadt, Germany). Oasis MCX (3 cc/60 mg) cartridges were purchased from Waters (Milford, MA, USA). The chemicals were all analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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