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Rpmi 1640 cell media

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RPMI 1640 is a cell culture medium designed to support the growth and maintenance of a variety of mammalian cell lines. It contains a balanced salt solution, amino acids, vitamins, and other nutrients necessary for cell proliferation.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Skov3, by American Type Culture Collection (1 mentions) Skov3 ip, by American Type Culture Collection (1 mentions) Facs lsr 2, by BD (1 mentions) Ultra low attachment plate, by Corning (1 mentions) Facs aria high speed cell sorter, by BD (1 mentions) Aldefluor reagent, by STEMCELL (1 mentions) Anti cd24 pacific blue, by Thermo Fisher Scientific (1 mentions) Anti cd24 pacific blue, by BD (1 mentions) Stempro msc sfm supplement, by Thermo Fisher Scientific (1 mentions) Anti cd44 alexa fluor 700, by BioLegend (1 mentions) Palladium chloride, by Maclin Biochemical Technology (1 mentions) Hoechst 33342, by Beyotime (1 mentions) Milli q plus system, by Merck Group (1 mentions) Fluo 4 am, by Beyotime (1 mentions)

Spelling variants of this product

RPMI 1640 (15891 citations) RPMI 1640 media (1491 citations) RPMI media (445 citations) 1640 media (43 citations) RPMI 1640 cell culture media (17 citations) DMEM and RPMI 1640 cell culture media (4 citations) Gibco RPMI 1640 cell culture media (2 citations)

5 protocols using rpmi 1640 cell media

1

Synthesis and Characterization of ART-ICG Conjugate

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ART (purified ≥98.0%) was obtained from Shanghai Ryon Biological Technology Co., Ltd, Shanghai, China. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), ICG and BSA (purified ≥98.0%) were purchased from Sigma-Aldrich (Co., St Louis, MO, USA). NH2-PEG2,000-RGD was purchased from Shaanxi BEO Biotechnology co., Ltd (Xi’an, China). Sulfo ICG-NHS ester was obtained from Xi’an Ruixi Biotechnology co., Ltd (Xi’an, China). RPMI 1640 cell media, FBS and PBS were obtained from Invitrogen (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) was supplied by Dojindo Laboratories (Kumamoto, Japan).
Ma Y., Liu X., Ma Q, & Liu Y. (2018). Near-infrared nanoparticles based on indocyanine green-conjugated albumin: a versatile platform for imaging-guided synergistic tumor chemo-phototherapy with temperature-responsive drug release. OncoTargets and therapy, 11, 8517-8528.
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2

Competitive Binding Assay for Radiolabeled BBN Analogue

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The protocol used was previously reported [24 ]. Specifically, [125I]-Tyr4-BBN(NH2) (PerkinElmer, Waltham, MA) was used as the competitor; cell media [500 mL RPMI 1640 cell media (Invitrogen, Carlsbad, CA) was supplemented with approximately 30 μM bovine serum albumin (BSA, 1 g, 15 μmol) and 20.6 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES, 2.46 g, 10.3 mmol), filtered through a 0.22 μm sterile filter, and pH adjusted to 7.4] was prepared for the study. Approximately 30,000 PC-3 cells were incubated at 37 °C, 95% humidity, and 5% CO2 for 45 min, with approximately 30,000 cpm [125I]-Tyr4-BBN(NH2) and varying concentrations of ReO- 5 (prepared by Method 1) in the modified cell growth media. The cells were washed with cold modified media to remove unbound activity. An LTi Multi-Wiper gamma counter (LTI Laboratory Technologies; Elburn, IL) was used to count the activity bound to the cells. The entire process was performed three times in duplicate. The IC50 value was calculated using GraFit (Erithacus Software, West Sussex, UK).
Demoin D.W., Dame A.N., Minard W.D., Gallazzi F., Seickman G.L., Rold T.L., Bernskoetter N., Fassbender M.E., Hoffman T.J., Deakyne C.A, & Jurisson S.S. (2016). Monooxorhenium(V) complexes with 222-N2S2 MAMA ligands for bifunctional chelator agents: Syntheses and preliminary in vivo evaluation. Nuclear medicine and biology, 43(12), 802-811.
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3

Metabolic Labeling of Ovarian Cancer Cells

Cited 1 time
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Serous ovarian cancer cell line SKOV3 and its high metastatic derivative SKOV3-ip were obtained from the American Type Culture Collection (ATCC). These two ovarian cancer cell lines were grown in RPMI1640 cell media (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Invitrogen). 293T cell line was obtained from the Institute of Cell Biology Academic Sinica (Shanghai, China). 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum. All cells were cultured with 1% penicillin/streptomycin at 37°C in a humidified 5% CO2 incubator. For stable isotope labeling experiments, cells were cultured for at least six doublings in the RPMI1640 medium with 10% fetal bovine serum, containing 20 mM L-glutamine (either 14N or amide-15N-Gln) to achieve complete incorporation of labeled N-glycans [41] (link). Amide-15N-Gln (98% purity) was purchased from Cambridge Isotopes, Inc. (Andover, MA, USA).
Zhang X., Wang Y., Qian Y., Wu X., Zhang Z., Liu X., Zhao R., Zhou L., Ruan Y., Xu J., Liu H., Ren S., Xu C, & Gu J. (2014). Discovery of Specific Metastasis-Related N-Glycan Alterations in Epithelial Ovarian Cancer Based on Quantitative Glycomics. PLoS ONE, 9(2), e87978.
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4

Characterization of CSC Markers in Cancer

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CSC markers were evaluated by multi-color flow cytometric analysis (FACS) on a BD FACS LSRII (BD Biosciences, San Jose, CA, USA), after cell staining with 0.5 μg per 100 μl of anti-CD24-Pacific Blue (eBioscience, San Diego, CA, USA) and anti-CD44-Alexafluor 700 (Biolegend, San Diego, CA, USA), 0.5 μg per 100 μl of anti-human-CD133-AF700 (MACS, Auburn, CA, USA) or ALDEFLUOR reagent (0.5 μg per 100 μl; Stem Cell Technologies, Vancouver, BC, CA). Data are represented as mean±s.d. *P<0.05; **P<0.01 were calculated by two-tailed Student's t-test (n=6; replicated 2 ×). CD24low/CD24high sub-populations of NCI-H292 cells were sorted utilizing anti-CD24-Pacific Blue antibody, using a BD FACSAria high-speed cell sorter (BD Biosciences). Similarly, CD133+ cells were sorted and collected in high fetal bovine serum (20%)-containing RPMI-1640 cell media (Gibco, Grand Island, NY) supplemented with 5% penicillin/streptomycin. Cells were centrifuged, washed 2 × with 1 × PBS, and resuspended in StemPro mesenchymal stem cell growth media supplemented with StemPro MSC SFM supplement (Gibco) and placed in ultra-low attachment plates (Corning) and monitored over the course of 7 days.
Little A.C., Sham D., Hristova M., Danyal K., Heppner D.E., Bauer R.A., Sipsey L.M., Habibovic A, & van der Vliet A. (2016). DUOX1 silencing in lung cancer promotes EMT, cancer stem cell characteristics and invasive properties. Oncogenesis, 5(10), e261-.
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5

Synthesis and Characterization of Palladium Nanoparticles

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Palladium chloride (59–60%) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China), hydrochloric acid (AR), hydrogen peroxide (30%) was purchased from Guangzhou brand reagent company (Guangzhou, China), oleic acid, oleylamine, benzyl alcohol (99%), absolute ethanol, and sucrose were obtained from Aladdin Biochemical Technology Co. (Shanghai, China). Fluo-4 AM, Hoechst 33342, Trypan Blue Solution (0.4%), HEPES buffer solution (pH = 7.4), PBS buffer solution (pH = 7.4) was bought from Beyotime Biotechnology (Shanghai, China). Roswell Park Memorial Institute (RPMI-1640) cell media were bought from Gibco. Jurkat T cells were donated by Southern Medical University. All other chemical reagents used in this experiment were analytically pure without further purification. Purified deionized water was prepared by the Milli-Q Plus system (Millipore, United States).
Fu D., Xie D., Wang F., Chen B., Wang Z, & Peng F. (2022). Mechanically Optimize T Cells Activation by Spiky Nanomotors. Frontiers in Bioengineering and Biotechnology, 10, 844091.
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