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Cd127 percp ef710 sb 199

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CD127-PerCP-ef710 (SB/199) is an antibody conjugate used for flow cytometry applications. It targets the CD127 antigen and is labeled with the PerCP-ef710 fluorescent dye.

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Lab products found in correlation

Gata 3 pe, by Thermo Fisher Scientific (2 mentions) Foxp3 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd3 bv605, by BioLegend (2 mentions) Cd11c pe cy7, by BioLegend (2 mentions) Cd16 32 fc block, by BD (2 mentions) Fortessa, by BD (2 mentions) Live dead fixable aqua stain, by Thermo Fisher Scientific (2 mentions) Foxp3 staining buffer kit, by Thermo Fisher Scientific (2 mentions) Siglecf e50 2440, by Thermo Fisher Scientific (2 mentions) Ly6g bv785, by BioLegend (2 mentions) Cd11b efluor450 m1 70, by Thermo Fisher Scientific (2 mentions) Cd19 bv711 1d3, by Thermo Fisher Scientific (2 mentions) Cd4 allophycocyanin efluor780 rm4 5, by Thermo Fisher Scientific (2 mentions) Klrg1 pe efluor610 2f1, by BioLegend (2 mentions) Cd11b allophycocyanin cy7, by BioLegend (2 mentions) Ly6c bv606, by BioLegend (2 mentions) Cd3 fitc 17a2, by Thermo Fisher Scientific (2 mentions) Cd45 bv711, by BioLegend (2 mentions)

2 protocols using cd127 percp ef710 sb 199

1

Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with BD Biosciences (Oxford, U.K.) mAbs: CD45-PerCP-Cy5.5 (30-F11), CD45.2-V450 (104), CD4-BV650 (RM4-5), CD3-FITC (17A2), CD11b-eFluor450 (M1/70), CD19-BV711 (1D3), SiglecF (E50-2440), CD103-PE-CF594 (M290), Ly6G-BV650 (1A8); eBioscience (Loughborough, U.K.) mAbs: CD4-allophycocyanin-eFluor780 (RM4-5); Invitrogen (Dublin, Ireland) mAbs: KLRG1-PE-eFluor610 (2F1) and CD127-PerCP-ef710 (SB/199); and BioLegend (London, U.K.) mAbs: CD45-BV711 (clone: 30-F11), CD3-BV605 (17A2), CD11b-allophycocyanin-Cy7 (M1/70), CD11c-PE-Cy7 (N418), Ly6G-BV785 (1A8), Ly6C-BV606 (HK1.4), and SiglecF-allophycocyanin (S1700L). Before surface staining, Fc receptors were blocked using Fc-Block CD16/32 (BD Biosciences), and cells were incubated with LIVE/DEAD Fixable Aqua stain (Molecular Probes, Invitrogen) to isolate dead cells. For staining of transcription factors, cells were fixed and permeabilized using the Foxp3 staining buffer kit (Invitrogen) and stained with mAbs: GATA3-PE (TWAJ) and Foxp3-PE-Cy7 (FJK-16s). For the detection of YFP, along with intracellular transcription factors from Rora-YFP mice, after surface markers and viability stain, cells were prefixed with 2% paraformaldehyde followed by Foxp3 staining buffer kit. Cells were analyzed using a BD Fortessa (BD Biosciences), and data were analyzed using FlowJo software (Tree Star, Ashland, OR), using appropriate controls.
Roberts J., Chevalier A., Hawerkamp H.C., Yeow A., Matarazzo L., Schwartz C., Hams E, & Fallon P.G. (2023). Retinoic Acid–Related Orphan Receptor α Is Required for Generation of Th2 Cells in Type 2 Pulmonary Inflammation. The Journal of Immunology Author Choice, 211(4), 626-632.
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2

Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained with BD Biosciences (Oxford, U.K.) mAbs: CD45-PerCP-Cy5.5 (30-F11), CD45.2-V450 (104), CD4-BV650 (RM4-5), CD3-FITC (17A2), CD11b-eFluor450 (M1/70), CD19-BV711 (1D3), SiglecF (E50-2440), CD103-PE-CF594 (M290), Ly6G-BV650 (1A8); eBioscience (Loughborough, U.K.) mAbs: CD4-allophycocyanin-eFluor780 (RM4-5); Invitrogen (Dublin, Ireland) mAbs: KLRG1-PE-eFluor610 (2F1) and CD127-PerCP-ef710 (SB/199); and BioLegend (London, U.K.) mAbs: CD45-BV711 (clone: 30-F11), CD3-BV605 (17A2), CD11b-allophycocyanin-Cy7 (M1/70), CD11c-PE-Cy7 (N418), Ly6G-BV785 (1A8), Ly6C-BV606 (HK1.4), and SiglecF-allophycocyanin (S1700L). Before surface staining, Fc receptors were blocked using Fc-Block CD16/32 (BD Biosciences), and cells were incubated with LIVE/DEAD Fixable Aqua stain (Molecular Probes, Invitrogen) to isolate dead cells. For staining of transcription factors, cells were fixed and permeabilized using the Foxp3 staining buffer kit (Invitrogen) and stained with mAbs: GATA3-PE (TWAJ) and Foxp3-PE-Cy7 (FJK-16s). For the detection of YFP, along with intracellular transcription factors from Rora-YFP mice, after surface markers and viability stain, cells were prefixed with 2% paraformaldehyde followed by Foxp3 staining buffer kit. Cells were analyzed using a BD Fortessa (BD Biosciences), and data were analyzed using FlowJo software (Tree Star, Ashland, OR), using appropriate controls.
Roberts J., Chevalier A., Hawerkamp H.C., Yeow A., Matarazzo L., Schwartz C., Hams E, & Fallon P.G. (2023). Retinoic Acid–Related Orphan Receptor α Is Required for Generation of Th2 Cells in Type 2 Pulmonary Inflammation. The Journal of Immunology Author Choice, 211(4), 626-632.
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