After 7 days on induction, HeLa cells grown on cover slips were washed and directly stained with AlexaFluor488 Goat anti Mouse IgM (Life Technologies, Carlsbad, CA, USA) to detect the presence of extracellular μΔCH1. Cells were then fixed in 4% paraformaldehyde: after permeabilization in 0.1% TX100 slides were stained with AlexaFluor546 Goat anti Mouse IgM (Life Technologies, Carlsbad, CA, USA) to detect intracellular material and Hoechst to visualize nuclei.
Alexafluor546 goat anti mouse igm
Manufactured by Thermo Fisher Scientific
Sourced in United States
AlexaFluor546 Goat anti Mouse IgM is a secondary antibody conjugated with the AlexaFluor546 fluorescent dye. It is designed to detect and bind to mouse IgM antibodies in various immunodetection techniques.
Automatically generated - may contain errors
2 protocols using alexafluor546 goat anti mouse igm
1
Detection of extracellular and intracellular μΔCH1
2
Immunostaining of Caenorhabditis elegans
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Day 3 adult worms were washed using EBT buffer (1× egg buffer, 20 mM sodium azide, and 0.2% Tween 20), decapitated to extract the germ line and intestine on a coverslip, and covered with a poly-lysine–treated slide. The microscope slide was then fast-frozen on dry ice, fixed in methanol for 2 min at −20°C, and washed with PBST followed by blocking with 10% donkey serum in PBST for 30 min. Samples were incubated overnight with anti–PGL-1 antibody (1:33; DSHB, K76) or anti-ATP5A (1:100; Abcam, ab14748) at room temperature. Alexa Fluor 546 goat anti-mouse IgM (1:500; Life Technologies, 2105681) and Alexa Fluor 546 goat anti-mouse IgG (1:500; Life Technologies, 1904466) were added for 2 hours. Slides were mounted with DAPI fluoromount-G (SouthernBiotech 0100-20).
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