Brain tissues were collected and immediately frozen in liquid nitrogen until analysis. Homogenization was performed in RIPA buffer with the addition of protease inhibitor cocktail. ADAR1 protein was detected in the brain tissues using Western blot as described previously.18 (link) In brief, 30ug of protein extract was loaded to each lane and separated on 8% polyacrylamide gel with 0.1% SDS. ADAR1 was detected with ADAR1 antibody clone 15.8.6 (Santa Cruz sc-73408) at 1:1,000 dilution.
Adar1 antibody clone 15
Manufactured by Santa Cruz Biotechnology
The ADAR1 antibody clone 15.8.6 is a research-use only product designed to detect the presence of the ADAR1 (Adenosine Deaminase Acting on RNA 1) protein in biological samples. ADAR1 is an enzyme involved in the post-transcriptional modification of RNA molecules. This antibody can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of ADAR1 in cells and tissues.
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Lab products found in correlation
3 protocols using adar1 antibody clone 15
1
ADAR1 Protein Detection in Brain Tissues
2
ADAR1 Protein Detection in Mouse Brain
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Before tissues were collected from the mice, whole body perfusion was performed with phosphate buffered saline (PBS) with heparin to remove blood from the organs. The tissues were immediately frozen in liquid nitrogen until analysis. For the brain sample collections, the cerebellum and olfactory bulb were removed prior to freezing. Homogenization was performed in RIPA buffer with the addition of protease inhibitor cocktail. ADAR1 protein in the brain tissues was detected using western blot as described previously [18 (link)]. In brief, 30 ug of protein extract was loaded to each lane and separated on 8% polyacrylamide gel with 0.1% SDS. ADAR1 was detected with ADAR1 antibody clone 15.8.6 (Santa Cruz sc-73408) at 1:1,000 dilution.
3
ADAR1 Protein Detection in Mouse Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before tissues were collected from the mice, whole body perfusion with PBS with heparin was performed to remove the blood from the organs. The tissues were immediately frozen in liquid nitrogen until analysis. For the brain sample collections, the cerebellum and olfactory bulb were removed prior to freezing. Homogenization was performed in RIPA buffer with the addition of protease inhibitor cocktail. ADAR1 protein in the brain tissues was detected by Western blot as described previously [31 (link)]. In brief, 30 μg of protein extract was loaded to each lane and separated on 8% polyacrylamide gel with 0.1% SDS. ADAR1 was detected with ADAR1 antibody clone 15.8.6 (Santa Cruz sc-73408) at 1:1000 dilution.
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