Eclipse ti e

Manufactured by Nikon

Sourced in Japan, United States, United Kingdom, Canada, Germany, Italy, France, Netherlands, Belgium, Azerbaijan

The Eclipse Ti-E is a high-performance research microscope platform from Nikon. It features motorized components for automated control and precise positioning of the sample, stage, and optics. The Eclipse Ti-E is designed for a wide range of advanced microscopy techniques, including live-cell imaging, high-resolution imaging, and quantitative analysis.

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Lab products found in correlation

Nis elements software, by Nikon (36 mentions) Csu x1, by Yokogawa (18 mentions) Nis elements ar software, by Nikon (18 mentions) Nis element, by Nikon (17 mentions) Volocity software, by PerkinElmer (15 mentions) Metamorph 7, by Molecular Devices (15 mentions) Fura 2 am, by Thermo Fisher Scientific (11 mentions) Metamorph software, by Molecular Devices (10 mentions) Orca flash 4, by Hamamatsu Photonics (10 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (10 mentions) Nis elements ar 4, by Nikon (9 mentions) Hoechst 33342, by Thermo Fisher Scientific (9 mentions) Cfi plan apochromat lambda, by Nikon (8 mentions) Ultraview, by PerkinElmer (8 mentions) Perfect focus system, by Nikon (8 mentions) Nis elements ar, by Nikon (8 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (8 mentions) Ixon ultra 888, by Oxford Instruments (8 mentions) Alexa fluor 488, by Thermo Fisher Scientific (7 mentions) Rp 40lp, by Warner Instruments (7 mentions)

736 protocols using eclipse ti e

Transient Expression of PvTET8-1 in Nicotiana benthamiana

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The 35S:PvTET8-1-GFP construct was electroporated into A. tumefaciens strain CV3010. Preparing A. tumefaciens (OD₆₀₀ 0.5) for infiltration into N. benthamiana epidermal cells were done on leaves from 4- to 6-week-old wild type plants as previously described (Norkunas et al., 2018 (link)). Leaves transformed with the vector harboring the 35S::GFP was used as the control. Images of transiently infected leaves were observed using confocal laser scanning microscopy (Nikon Eclipse Ti-E) after 72 h of infiltration. The subcellular localization of PvTET8-1 was determined in P. vulgaris root hairs from 4-day-old transgenic hairy roots, generated with A. rhizogenes carrying 35S::PvTET8-1-GFP. Root hairs were observed using confocal laser scanning microscopy (Nikon Eclipse Ti-E).

Parra-Aguilar T.J., Sarmiento-López L.G., Santana O., Olivares J.E., Pascual-Morales E., Jiménez-Jiménez S., Quero-Hostos A., Palacios-Martínez J., Chávez-Martínez A.I, & Cárdenas L. (2023). TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions. Frontiers in Plant Science, 14, 1152493.

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Fluorescent Staining and Imaging of Cell Viability

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To image cell viability, 100 μL of 0.05% Calcein AM in sterile PBS (Life Technologies LIVE/DEAD® Cell Viability Assay) were added to wells and kept for 30 minutes before imaging on a Nikon Eclipse Ti-E with Andor Zyla camera.
LiMMEs were fixed using 120 μL of 4% Paraformaldehyde (PFA) in PBS for at least 2 hours to allow diffusion. Phalloidin-FITC (P5282 Sigma) − 0.1 mg/mL in methanol – was diluted 1:150 in PBS and wells were treated with 100 μL for 30 minutes, each. A 1:1000 DAPI (D1306 ThermoFisher Scientific, 1 mg/mL in diH₂O) to PBS ratio was used to treat LiMMEs for 10 minutes to label nuclei. LiMMEs were rinsed 3 times with PBS every 30 minutes for 3 hours, and Phalloidin/DAPI images were obtained using a Nikon Eclipse Ti-E microscope with a C2 confocal scanning system.

Rubiano A., Delitto D., Han S., Gerber M., Galitz C., Trevino J., Thomas R.M., Hughes S.J, & Simmons C.S. (2017). Viscoelastic properties of human pancreatic tumors and in vitro constructs to mimic mechanical properties. Acta biomaterialia, 67, 331-340.

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Quantifying DNA Damage Repair Foci

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For ionizing radiation (IR) induced U2OS-YFP-RAD52 foci, cells were grown on IBIDI 35 mm μ-dishes and incubated overnight. Next day, cells were irradiated (10 Gy) and 2 h post irradiation fixed with 4% formaldehyde at room temperature for 10 min, washed three times with PBS and nuclei were stained using DAPI (Panreac AppliChem). For hydroxyurea (HU) induced U2OS-YFP-RAD52 foci, cells were grown on IBIDI eight-well μ-slides and incubated overnight. Next day, cells were treated with 2 mM HU (sigma-Aldrich) for 4 h and fixed as described above. The slides were viewed at 600× magnification on Nikon fluorescence microscope (EclipseTi-E) using high-throughput mode with 100 images captured for one sample. Nd files from Nikon Eclipse Ti-E was used in automated analysis using CellProfiler 2.3 (53 (link)). DAPI channel was used for cell segmentation. Automatic identification of the RAD52 foci was done based on the intensity and size. At least 1000 cells were quantified in each condition. Statistical analysis was performed in GraphPad Prism 7.

Stefanovie B., Hengel S.R., Mlcouskova J., Prochazkova J., Spirek M., Nikulenkov F., Nemecek D., Koch B.G., Bain F.E., Yu L., Spies M, & Krejci L. (2019). DSS1 interacts with and stimulates RAD52 to promote the repair of DSBs. Nucleic Acids Research, 48(2), 694-708.

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Phase Separation of Fusion Proteins

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The phase diagrams of the different fusion proteins were obtained by analyzing 1 µM protein solutions by bright-field microscopy (Eclipse Ti-E, Nikon) using a 60x oil objective (FI Plan Apo Lambda NA 1.4, Nikon). To this aim, the fusion proteins were dissolved from stock solutions into 25 mM Tris buffer, 10 mM NaCl, pH 7.5, and allowed to phase separate for 10 min. After analyzing the presence of condensates, the salt concentration was progressively increased to 100 mM, 300 mM, and 500 mM, and images were acquired after 10 min equilibration after each change of salt concentration.
To estimate the c_sat of the different fusion proteins, samples were prepared at different protein concentrations (from 100 nM to 1 µM) at 25 mM Tris, 20 mM NaCl, pH 7.5, and allowed to equilibrate for 10 min. The presence of condensates was analyzed by bright-field microscopy (Eclipse Ti-E, Nikon) using a 60x oil objective (FI Plan Apo Lambda 60× Oil, Nikon).
All the measurements were performed in a 384-well plate (MatriPlate 384-Well Plate, Glass Bottom, Brooks).

Gil-Garcia M., Benítez-Mateos A.I., Papp M., Stoffel F., Morelli C., Normak K., Makasewicz K., Faltova L., Paradisi F, & Arosio P. (2024). Local environment in biomolecular condensates modulates enzymatic activity across length scales. Nature Communications, 15, 3322.

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Fluorescence Imaging of Microbial Colonies

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Fluorescence images of colonies were acquired after 7-8 days of growth using a 1X objective on a Nikon Eclipse Ti-E inverted fluorescence microscope equipped with a DU897 electron multiplying charged couple device (EMCCD) camera (Andor) using µManager v. 1.4 (49) , or a Nikon TE-2000 or Zeiss Axio Zoom.V16 microscope. Colonies sandwiched between two agar surfaces and the colonies in the corresponding control experiments were imaged after 7 days at 4 °C. Colonies that did not show sufficient fluorescence at this point, were imaged again after 26 more days at 4 °C.
Edges of colonies were imaged with a 20X objective on a Nikon Eclipse Ti-E inverted fluorescence microscope equipped with a DU897 camera (Andor) using µManager v. 1.4.

Aranda-Díaz A., Rodrigues C., Grote A., Sun J., Schreck C.F., Hallatschek O., Souslov A., Möbius W., & Huang K.C. (2021). Bacterial filamentation drives colony chirality.

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Fluorescence Imaging of Microbial Colonies

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Fluorescence images of colonies were acquired following growth using a Nikon Eclipse Ti-E inverted fluorescence microscope equipped with a DU897 electron-multiplying charge-coupled device (EMCCD) camera (Andor) using μManager v. 1.4 (52 (link)) or a Nikon TE-2000 or Zeiss Axio Zoom.V16 microscope. Colonies sandwiched between two agar surfaces and the colonies in the corresponding control experiments were imaged after 7 days at 4°C. Colonies that did not show sufficient fluorescence at this point were imaged again after 26 more days at 4°C.
Edges of colonies were imaged with a 20X objective on a Nikon Eclipse Ti-E inverted fluorescence microscope equipped with a DU897 camera (Andor) using μManager v. 1.4.

Aranda-Díaz A., Rodrigues C., Grote A., Sun J., Schreck C., Hallatschek O., Souslov A., Möbius W, & Huang K.C. (2021). Bacterial Filamentation Drives Colony Chirality. mBio, 12(6), e01542-21.

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Live-Cell Fluorescence Imaging of ERGIC

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Cells were plated in Lab-Tek II chambered coverglass and transfected as described above. For cells transfected with HaloTag-Sec23A, 0.2 μM of JF635 HaloTag ligand was added to the cell culture medium 1 hr before imaging. After incubation at 37°C for 30 min, the cells were rinsed with the normal cell culture medium for 5 min × 6 times. Prior to imaging, 25 mM HEPES was added to the cell culture medium to maintain the pH in the ambient environment.
Live-cell fluorescence microscopy was performed on an Olympus IX73 inverted epifluorescence microscope with a water-immersion objective (Olympus, UPLSAPO60XW, NA 1.2) and a mercury lamp, or a Nikon Eclipse Ti-E inverted fluorescence microscope with an oil-immersion objective (Nikon CFI Plan Apochromat λ 100×, NA 1.45) with 488-nm, 560-nm, and 647-nm lasers modulated by an acousto-optic tunable fiber (AOTF, Gooch & Housego, 97–03151-01). Cells were imaged at 2–20 frames per second (fps) at room temperature to moderately slow down the motion of the fast-moving t-ERGIC. Concurrent multi-color imaging was achieved by modulating the AOTF to allow frame-synchronized alternating excitation at 488, 560, and 647 nm (Yan et al., 2020 (link)) with a multi-bandpass filter cube (Semrock Di01-R405/488/561/635 and Chroma ZET405/488/561/640m).

Yan R., Chen K., Wang B, & Xu K. (2022). SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport. Developmental cell, 57(4), 512-525.e8.

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Live Cell Imaging of Transfected Cells

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Cells were cultured on glass bottom dishes (801001; NEST). 24 h after transfection of plasmids, cells were cultured in HBSS medium and imaged at 37°C and 5% CO₂ using a 100× objective (CFI Plan Apochromat Lambda, NA 1.45; Nikon) with immersion oil on an inverted fluorescence microscope (Eclipse Ti-E; Nikon) with a spinning-disk confocal scanner unit (UltraView; PerkinElmer). Images were taken every 5 s for 1 h. The images were analyzed with Volocity software (PerkinElmer).

Liu N., Zhao H., Zhao Y.G., Hu J, & Zhang H. (2021). Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. The Journal of Cell Biology, 220(7), e202012091.

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GUS and Mycorrhization Assays in Plants

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Composite plants harboring pPvTET8-1::GFP-GUS and empty vector control were collected after 7, 14 and 21 dpi for nodulation and 14 dpi for mycorrhization assays. Histochemical staining for GUS activity was done as described by Jefferson et al. (1987) (link) and images were acquired with an inverted microscope (Nikon Eclipse Ti-E) at 10-40X magnification (Jefferson et al., 1987 (link)). For the mycorrhization assay, dual GUS-WGA Alexa Fluor^® 488 staining was performed for promoter activity and mycorrhizal colonization, respectively (Kuhn et al., 2010 (link)). Colonized roots were fixed with 50% EtOH for 24 h, and then cleared in 20% KOH for 72 h. The fungal cell walls were stained with 0.2 µg/mL WGA-Alexa Fluor^® 488 (Invitrogen^®) according to Manck-Gotzenberger and Requena (2016) (link). For each construct, at least 30 root segments were analyzed (Manck-Gotzenberger and Requena, 2016 (link)).

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Visualizing Dam Methylation in E. coli

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The method is described in detail elsewhere (Shao et al., 2015) . The culture of cells with Dam MTase gene deleEon (E. coli K-12 MG1655) was grown at M9 to OD 600 = 0.4 with the 10 mM MgSO 4 and 0.2% maltose. The cells were mixed with bacteriophage λvir whose DNA is fullymethylated by Dam MTase and incubated for 5 minutes. A 1 µl of the mixture was placed on an agarose pad containing M9 medium. The Eme-lapse filming was performed using a Nikon Eclipse Ti-E inverted microscope. The filming was carried out in transmihed light (TL) and mKO2 fluorescence channels for the bacteriophage DNA visualizaEon. VisualizaEon was performed due to the detecEon of SeqA::mKO2 protein which specifically binds methylated or hemimethylated by Dam MTase DNA. The disEnct foci were visualized using the z-stacks. Each field of view was imaged every 15 minutes for more than 150 minutes.

Кириллов А.И., Morozova N., Polinovaskaya V., Smirnov S., Ходорковский М.А., Zeng L., Ispolatov Y., & Severinov K. (2022). Cells with Stochastically Increased Methyltransferase to Restriction Endonuclease Ratio Provide an Entry for Bacteriophage into Protected Cell Population.

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