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2 protocols using d glucose

1

Bacterial Growth Media Preparation

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Brain heart infusion (BHI), sheep blood agar (SBA), and THY were from BD Biosciences (San Jose, CA) or RPI. Kanamycin and D-glucose were purchased from Research Products International (RPI; Mt. Prospect, IL). Glycine was purchased from AMERSCO (Solon, OH). Unless otherwise specified, all reagents were purchased from Sigma Aldrich. M9 minimal medium was prepared as previously described (Miller, 2010 (link)).
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2

Biodegradable PLGA Nanoparticle Synthesis

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PLGA polymer [Resomer® 503, 50:50 with inherent viscosity 0.32 – 0.44 dl/g] was obtained from Evonik Industries AG (Darmstadt, Germany). Acetone and N, N dimethylformamide (DMF) were purchased from Fisher Chemicals (Fair Lawn, NJ, USA). Nile Red and Trypsin-EDTA solution were obtained from MilliporeSigma (St. Louis, MO, USA). SnakeSkin® pleated dialysis tubing with a 7000 Da molecular weight cutoff was purchased from ThermoFisher Scientific (Rockford, IL, USA). Cellosolve® Acetate (2-ethoxyethyl acetate) was obtained from AlfaAesar (Ward Hill, MD, USA). D-Glucose was obtained from Research Products International (RPI) (Mt. Prospect, IL, USA). Whatman filter paper was purchased from Global Life Sciences Solutions USA LLC (Pittsburgh, PA, USA).
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