Demineralization solution (2mM Ca(NO3)2, 2mM K3PO4, 75 mM CH3COOH; pH 4.4) was prepared according to the method proposed earlier.28 (link),29 (link) The following chemicals were used without further purification: calcium nitrate hydrate (Ca(NO3)2, Sigma-Aldrich), potassium phosphate tribasic (K3PO4, Sigma-Aldrich), and acetic acid (CH3COOH, Sigma-Aldrich).
K3po4
Manufactured by Merck Group
Sourced in Germany
K3PO4 is a chemical compound that serves as a buffering agent and pH regulator. It is commonly used in various laboratory applications to maintain a specific pH range. The product's core function is to stabilize the pH of solutions and facilitate controlled chemical reactions.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (2 mentions)
Ammonium formate, by Merck Group (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ca no3 2, by Merck Group (1 mentions)
Ch3cooh, by Merck Group (1 mentions)
Acetic acid ch3cooh, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions)
Anhydrous sodium sulphate, by Merck Group (1 mentions)
Linalool, by Merck Group (1 mentions)
Butylated hydroxytoluene, by Merck Group (1 mentions)
Muller hinton agar, by Bio-Rad (1 mentions)
Red violet bile glucose medium, by Thermo Fisher Scientific (1 mentions)
Bis trimethylsilyl tri fluoroacetamide, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Kh2po4, by Kanto Chemical (1 mentions)
Isocitrate, by Merck Group (1 mentions)
Isocitrate dehydrogenase, by Merck Group (1 mentions)
12 protocols using k3po4
1
Demineralization Solution Preparation
2
Antimicrobial activity assessment
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Anhydrous sodium sulphate, linalool ((±)-3,7-dimethyl-1,6-octadien-3-ol), butylatedhydroxytoluene (BHT), streptomycin, thiazolyl blue tetrazolium bromide (MTT), and potassium phosphate buffer (K3PO4) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Muller–Hinton agar was purchased from Bio-Rad, Marnes-la-Coquette, France. Agar plates of MH agar and red-violet bile glucose medium were obtained from Oxoid Ltd., Basingstoke, UK.
For extraction and derivatization, ethanol, pyridine, and bis-(trimethylsilyl) tri-fluoroacetamide (BSTFA) were purchased from Sigma-Aldrich (Steinheim, Germany).
For extraction and derivatization, ethanol, pyridine, and bis-(trimethylsilyl) tri-fluoroacetamide (BSTFA) were purchased from Sigma-Aldrich (Steinheim, Germany).
3
Nanomaterial-enabled DNA Detection
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Single-stranded deoxyribonucleic acid (ss-DNA), double-strand DNA (ds-DNA), low molecular weight DNA (lm-DNA), MnO2, K3PO4, and phosphate-buffered saline (PBS) are all purchased from Sigma-Aldrich (China). Graphite with purity higher than 98.3% was obtained from Sinocarbon Materials Technology Co., Ltd., China. Experimental used water is deionized water ((DI H2O), 18.2 MΩ/cm, ELGA Lab Water, England).
4
Photoanode Fabrication for Water Oxidation
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According to the previously reported method,34 (link) Ti(OCH(CH3)2)4 treatment was carried out by dipping the PbFeO2F/FTO electrode in an ethanol solution of 0.1 M Ti(OCH(CH3)2)4 (>97%, Kanto Chemical), followed by drying on a hotplate at ∼423 K. The procedure was repeated five times. Finally, the electrode was heated in air at 573 K for 1 h. Cobalt phosphate (Co–Pi) cocatalyst, known as a water-oxidation promoter,35 (link) was then electrodeposited onto the TiO2/PbFeO2F/FTO electrode.35,36 (link) A three-electrode cell was used with the TiO2/PbFeO2F/FTO as the working electrode, a Ag/AgCl electrode as the reference electrode and Pt wire as the counter electrode. An electrochemical bias of +1.0 V vs. Ag/AgCl was applied to the working electrode in 0.1 M potassium phosphate buffered at pH 7 and containing 0.5 mM cobalt nitrate (98%, FUJIFILM Wako Pure Chemical) until the charge passing through the outer circuit reached 100 mC unless otherwise stated. The pH of the phosphate solution was controlled by mixing KH2PO4 (>98.0%, Kanto Chemical), K2HPO4 (>98.0%, Kanto Chemical) and/or K3PO4 (≥98%, Sigma-Aldrich), where the concentration was maintained at 0.1 M in total.
5
Quantitative Analysis of Novel Psychoactive Substances
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alpha-PBP and alpha-PEP were provided by the Bavarian State Criminal Police Office (Munich, Germany). NADP-Na2, acetonitrile (LC-MS grade), and methanol (LC-MS grade) were obtained from VWR (Darmstadt, Germany), MgCl2, K2HPO4, K3PO4, superoxide dismutase, isocitrate dehydrogenase, isocitrate, ammonium formate, ammonium acetate, and formic acid from Sigma (Taufkirchen, Germany). Water was purified with a Millipore filtration unit (18.2 Ω × cm water resistance). pHLM (pool of 25 donors, 20 mg microsomal protein/mL) were obtained from Corning (Amsterdam, The Netherlands). After delivery, the pHLM were thawed at 37 °C, aliquoted, snap-frozen in liquid nitrogen, and stored at −80 °C until use.
6
Protein Extraction from Frozen Tissues
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Fresh frozen tissue samples were homogenized in 5 volumes (µl) of homogenization buffer (20 mM Tris-base, pH 7.6, 1:100 EDTA-free protease inhibitor [Sigma-Aldrich), 1:10 EDTA-free phosphatase inhibitor (PhosSTOP; Roche)] per mg of tissue at 4°C using a hand-held tissue grinder with metal-free polypropylene probes (Kontes Pellet Pestle®, Sigma-Aldrich). Homogenates were aliquoted and stored at −80°C, or were centrifuged (16 000g, 30 min, 4°C) and the supernatant and pellet collected, aliquoted and stored at −80°C. Protein concentrations within homogenates and supernatants were quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). Supernatant and pellet tissue fractions will subsequently be referred to as soluble and insoluble tissue extracts, respectively. Measures were undertaken to prevent metal contamination of tissue extracts, including the use of plastic forceps for handling tissues, and the removal of trace metal impurities from buffers and glassware using Chelex resin (Bio-Rad) and a metal-chelating rinse solution (0.37% EDTA in 2.25 mM K3PO4; Sigma-Aldrich), respectively. These measures were also incorporated into native isoelectric focusing experiments and SOD1 specific activity assays.
7
Synthesis of Functionalized Organic Compounds
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Pd(OAc)2 (Sigma, 97%), SPhos (TCI Europe, >98%), K3PO4 (Sigma, ≥98%), ICl (Sigma, 1 M solution in CH2Cl2), Pd(PPh3)2Cl2, (Sigma, 98%), CuI (Sigma, 98%), ethynyltrimethylsilane, (Sigma, 98%), tetrabutylammonium fluoride (Sigma, 1 M THF), o-xylene (Sigma, anhydrous 97%), FeCl3, (Sigma, anhydrous for synthesis), CH3NO2 (Sigma, absolute, over molecular sieve, ≥98.5%), B2Pin2, (Combi-Blocks, 98%), Pd(dppf)Cl2 (Sigma, not specified), KOAc (Sigma, anhydrous ≥99%), 1,4-dioxane (Thermo Scientific Chemicals, anhydrous 99.8%), Pd(PPh3)4 (Sigma, 99%), 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (Thermo Scientific Chemicals, ≥98%), triflic acid (Sigma, ≥ 99%), AgNO3 (Sigma, ≥99%), and tetra-n-butylammonium hexafluorophosphate (Sigma, 98%) were used as received. 1,4-diiodo-2,5-bis(trimethylsilyl)benzene51 (link) and 2,5-diphenyl-3,4-di(4-tert-butylphenyl)-cyclopentadien-1-one52 (link) were synthesized according literature procedures. Solvents were purchased from SDS Carlo Erba, Aldrich, and Fisher Scientific and were used as received. For synthesis, unstabilized CH2Cl2 (CaH2, N2), toluene (K/benzophenone, N2), THF (K/benzophenone, N2), Et3N (CaH2, N2), and Et2O (CaH2, N2) were distilled before use.
8
Synthesis of Dental Biomaterial Precursors
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Calcium hydroxide (98%, ACROS Organics B.V.B.A, Fair Lawn, NJ, USA), calcium nitrate tetrahydrate (Ca(NO3)2 × 4 H2O, 99%, Merck KGaA, Darmstadt, Germany), deionized water (Millipore®, Merck KGaA, Darmstadt, Germany), dimethyl sulfoxide (>99.5%, Sigma-Aldrich Chemie GmbH, Munich, Germany), (2-((2-(ethoxycarbonyl) allyl)oxy)ethyl)phosphonic acid (ECPA, Ivoclar Vivadent AG Schaan, Liechtenstein), ethanol (99.9%, VWR International GmbH, Radnor, PA, USA), hydrochloric acid, (HCl, 0.1 mol/L Titrisol®, Merck KGaA, Darmstadt, Germany), isopropanol (analytical grade, Thermo Fisher Scientific GmbH, Dreieich, Germany), N,N′-(propane-1,3-diyl)bis(N-ethylacrylamide) (Ivoclar Vivadent AG Schaan, Liechtenstein), potassium phosphate tribasic (K3PO4, ≥98%, Sigma-Aldrich Chemie GmbH, Munich, Germany), propylene glycol (99%, ACROS Organics B.V.B.A, Fair Lawn, NJ, USA), sodium hydroxide (NaOH, 0.1 mol/L Titrisol®, Merck KGaA, Darmstadt, Germany) (2,4,6-trimethylbenzoyl)diphenylphosphine oxide (97%, Sigma-Aldrich Chemie GmbH, Munich, Germany) were used as received.
9
Switchgrass Characterization and Catalyst Preparation
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Switchgrass was crushed and sieved into small particles (300–600 μm). The ultimate and proximate analyses of switchgrass were carried out; the moisture, ash, volatile matter, and fixed carbon contents are 5.1, 6.3, 76.9, and 11.7 wt.%, respectively, and the higher heating value (HHV) is 19.6 MJ/kg. The C, H, N, S, and O contents were determined using a CHNS/O analyzer, Perkin-Elmer 2400Series II, and the values are 47.9, 6.2, 0.8, 0.1, and 38.7 wt.% (O content was calculated by difference), respectively. Bentonite and K3PO4 were purchased from Sigma-Aldrich, Canada Co. Clinoptilolite was purchased from Bear River Zeolite Co., United States.
10
Cassava Starch Characterization and Applications
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Cassava starch (food grade, approximately 82% amylopectin and 18% amylose) was purchased from a local market (Salaya, Nakhon Pathom, Thailand). The used as-received chemicals were potassium phosphate (K3PO4; Sigma-Aldrich, Munich, Germany), dimethyl sulfoxide (DMSO; AR grade, Central Drug House Ltd., Delhi, India), methyl laurate (Tokyo Chemical Industry, TCI, Tokyo, Japan), chloroform-d (Cambridge Isotope Laboratories, Inc., Cambridge, UK), ethanol, and chloroform (AR grade, RCI Labscan limited, Bangkok, Thailand). Deionized (DI) water was employed as a solvent.
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