Echocardiography was performed at P5 and P10 using a Vevo3100 imaging system (VisualSonics) with MX550 (for P10) or MX700 (for P5) probes. Two-dimensional-guided M-mode images of the short axis at the papillary muscle level were recorded. Data were analyzed by VevoLab 3.2.5 software using Auto-LV technology to minimize the variability between the measurement and confirmed by an experienced researcher. For electrocardiography (ECG) measurements the PR interval was measured from the beginning of the P-wave to the beginning of the QRS complex; QRS duration was measured from the first deflection of the Q-wave to the nadir of the S-wave (defined as the point of minimum voltage in the terminal portion of the QRS complex); the R-R interval was obtained as the average R-R interval over the sampling period.
Mx550
Manufactured by Fujifilm
Sourced in Canada
The MX550 is a compact, multifunction medical imaging device designed for diagnostic applications. It offers high-quality image capture and processing capabilities, catering to the needs of healthcare professionals. The core functionality of the MX550 is to provide reliable and efficient medical imaging solutions.
Automatically generated - may contain errors
4 protocols using mx550
1
Echocardiography and Electrocardiography in Mice
2
Echocardiographic Assessment of Post-MI
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Echocardiography was performed 21 days after MI using a Vevo 3100 imaging system (VisualSonics) with the MX550 or MX700 probe. Parasternal long-axis views, short-axis views, and 2-dimensional-guided M-mode images of the short axis at the papillary muscle level were captured. To minimize variability between the measurements, all echocardiographic measurements were analyzed by VevoLab 3.2.5 software using artificial intelligence–based Auto-LV technology.
3
Echocardiographic Evaluation of Mice
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Transthoracic M-mode and Doppler echocardiographic examinations were performed using the Vevo 3100 with a 40 MHz transducer (MX550S; VisualSonics, Toronto, Canada) as previously described [38 (link)]. Mice were placed on a heating pad and a nose cone with 2% isoflurane in 100% oxygen was applied. The temperature was maintained at 36.5 to 37.5 °C. Ultrasound gel was placed on the chest of the anesthetized mouse. The ultrasound probe was placed in contact with the ultrasound gel and scanning was performed over 30 min. The temperature and heart rate were constantly monitored during the scanning. M-mode images were used to measure LV chamber size and wall thickness, among other parameters.
4
Comprehensive Cardiac Functional Assessment
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Transthoracic 2D echocardiography was used for cardiac functional assessment 1 week prior to animal euthanasia. Animals were anesthetized by isoflurane (1–2%) and recordings were taken using Vevo 3100 high-resolution imaging system, 40 MHz transducer (MX550S; Visual Sonics). Visual Sonics VevoLab software was used for assessment of the cardiac images. Left ventricular interior volumes and left ventricular internal diameters (LVID) were determined from m-mode images taken at the mid-papillary level. Left ventricular ejection fraction (%EF) was calculated using the equation % EF = [(LVEDV - LVESV)/LVEDV] × 100. Left ventricular mass (corrected, mg) was calculated with 1.05 × [(LVID; d + LVPW; d + IVS; d)^3-LVID; d^3] × 0.8. The transmitral filling pattern was assessed by pulsed-wave Doppler imaging to determine diastolic function. The E/A ratio represents early transmitral wave (E-wave) followed by the late filling wave due to atrial “kick” (A-wave). Tissue Doppler imaging was used to describe the motion of the mitral annulus (E’ and A’).
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