Ascend aeon wb 400

The structure of
the synthesized IL was confirmed using a Bruker Ascend Aeon WB 400
(Bruker BioSpin AG, Fällanden, Switzerland) nuclear magnetic
resonance (NMR) spectrometer. The working frequencies were 400.21
MHz for ¹H, 100.64 MHz for ¹³C, and 162.01 MHz
for ³¹P. DMSO-d₆ was used as
a solvent, and the data were processed using Bruker Topspin 3.5 software.
The NMR resonance lines assignment is given below.
¹H NMR (400.21 MHz, DMSO-d₆) δ/ppm:
7.45 (1H, s, O–CH=CH), 6.64–6.30 (1H, m, CH–CH=C),
6.34–6.33 (1H, m, CH=CH–CH), 2.22–2.14
(8H, m, 4× PCH₂), 1.45–1.21 (48H, m, −CH₂−), 0.86–0.81 (12H, t, −CH₃). ¹³C NMR (100.63 MHz, DMSO-d₆) δ/ppm: anion (162.72, 154.09, 142.66, 111.72, 111.09), cation
(31.92, 30.40, 29.69, 29.59, 22.71, 22.42, 18.37, 17.90, 14.44, 14.38). ³¹P NMR (162.01 MHz, DMSO-d₆) δ/ppm:
33.51. The ¹H, ¹³C, and ³¹P NMR spectra
of [P_6,6,6,14][FuA] in DMSO-d₆ are shown in the Supporting Information (Figures S1–S3).
Fourier Transform infrared spectroscopy
(FT-IR, Nicolet iS10) was
used to further characterize the structure of the synthesized IL.
Morphology and the surface roughness of TNAs were characterized by
field-emission scanning electron microscopy (FESEM, JSM-7800F PRIME)
and AFM (Bruker ICON). X-ray photoelectron spectroscopy (XPS, PHI
QuantERA II) was used to analyze the percentage of Cyt c on TNAs.

The lithium salt and ionic liquid were characterized using Bruker Ascend Aeon WB 400 (Bruker BioSpin AG, Fällanden, Switzerland) NMR spectrometer. NMR spectra of the electrolytes were recorded with CDCl₃ solvent as an external lock. The working frequency was 100.63 MHz for ¹³C, 162.01 MHz for ³¹P, 128.40 MHz for ¹¹B and 155.56 MHz for ⁷Li. Data were processed using Bruker Topspin 3.5 software.