Specific siRNA for MTA1 (sc-35981) was purchased from Santa Cruz and used at a final concentration of 100 nM. Control siRNA (D-001810-10) was purchased from GE Dharmacon. Lipofectamine RNAiMAX (Invitrogen) was used for siRNA Transfections carried out in 6-well plate using with Opti-MEM (Invitrogen) according to the manufacturer’s protocol. Plasmids indicated were transiently transfected into the cells in 6-well plate using Lipofectamine LTX (Invitrogen) with Opti-MEM in accordance with the manufacturer’s instructions.
Control sirna d 001810 10
Manufactured by Horizon Discovery
Control siRNA (D-001810-10) is a non-targeting small interfering RNA (siRNA) designed for use as a negative control in siRNA experiments. It does not target any known gene in the human, mouse, or rat genome.
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Lab products found in correlation
3 protocols using control sirna d 001810 10
1
siRNA Knockdown of MTA1 in Cells
2
Cloning and Expression of JNK1 Isoforms
3
siRNA Transfection of hESCs
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H1 hESCs were maintained on an inactivated mouse embryonic feeder layer (MEF) in DMEM/F12 supplemented with 20% KSR, 1× MEM non-essential amino acids solution, 1× Glutamax, 55 μM 2-mercaptoethanol and 4 ng/ml bFGF. For CLIP and transfection of siRNAs, H1 cells were transferred to Geltrex-coated plates with MEF-conditioned media supplemented with 50 ng/ml bFGF and passaged twice. H1 cells were transfected with siRNAs as described previously with minor modifications (32 (link)). Briefly, hESCs were treated with 10 μM Y-27632 for 2 hr before dissociation with TrypLE. Approximately 4 × 106 hESCs were washed once with Opti-MEM and resuspended in 600 μl RNAiMAX/siRNA complex in Opti-MEM. After 15 min, the transfected cells were plated onto Geltrex-coated 10 cm plates with 10 μM Y-27632. Thirty microliters of RNAiMAX and 60 pmol of siRNAs were used for the transfection. After 24 hr, the transfection process was repeated. The cells were harvested after another 24 hr. Control siRNA (D-001810-10) and siL1TD1 (L-017976-01) were purchased from Dharmacon.
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