For AFM, bacterial cells or OMV preparations were diluted with ultrapure water (Millipore) and placed onto a freshly cleaved mica surface. Samples were incubated for 5 min at room temperature, washed with ultrapure water, and then placed in a desiccator for ~2 h in order to dry. The samples were finally magnified through a Nanoscope V Atomic Force Microscope (Bruker AXS GmbH, Karlsruhe, Germany), using tapping mode. Final images were plane fitted in both the x and y axes and are presented in amplitude mode.
Nanoscope 5 atomic force microscope
Manufactured by Bruker
Sourced in Germany
The Nanoscope V Atomic Force Microscope is a high-resolution imaging instrument that uses a sharp tip to scan the surface of a sample. It measures the small forces between the tip and the sample, generating a detailed topographic map of the surface at the nanometer scale.
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Lab products found in correlation
5 protocols using nanoscope 5 atomic force microscope
1
Atomic Force Microscopy of Bacterial Samples
2
Atomic Force Microscopy of S. aureus
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Atomic force microscopy (AFM) analysis of S. aureus MSSA476 cultivated on Luria-Bertani (LB) agar (LA), brain-heart infusion (BHI) agar, and blood agar were carried out as described previously (Thay et al., 2013 (link)). Briefly, bacterial cells were suspended in ultrapure water and placed on a freshly cleaved mica surface. The samples were incubated for approximately 5 min at room temperature and blotted dry before being placed into a desiccator. Representative images were collected by a Nanoscope V Atomic Force Microscope (Bruker AXS, Germany).
3
Bacterial Cell Topography via AFM
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For AFM, bacterial cells were diluted with ultrapure water (Millipore) and placed onto a freshly cleaved mica surface. Samples were incubated for 5 min at room temperature, washed with ultrapure water, and then placed in a desiccator for ~2 h in order to dry. The samples were finally magnified through a Nanoscope V Atomic Force Microscope (Bruker AXS GmbH, Karlsruhe, Germany), using tapping mode. Final images were plane fitted in both the x- and y-axes and are presented in amplitude mode.
4
AFM Imaging of Outer Membrane Vesicles
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For AFM, samples of OMVs were diluted with ultrapure water (Millipore) and placed onto a freshly cleaved mica surface. Samples were incubated for 5 min at room temperature, washed with ultrapure water, and then placed in a desiccator for ~2 h in order to dry. The samples were finally magnified through a Nanoscope V Atomic Force Microscope (Bruker AXS GmbH, Karlsruhe, Germany), using tapping mode. Final images were plane fitted in both the x and y axes and are presented in amplitude mode.
5
Bacterial Cell Surface Imaging by AFM
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Washed and concentrated bacterial cultures were placed on a freshly cleaved ruby red mica (Goodfellow Cambridge Ltd, Cambridge), incubated for 5 min at room temperature and blotted dry before placement in a dessicator for at least 2 h. Images were collected within a Nanoscope V atomic force microscope (Bruker software) using ScanAsyst in air with ScanAsyst cantilevers, at a scanrate of ~0.9–1 Hz. The final images were flattened and/or planefitted in both axes using Bruker software and presented in amplitude (error) mode.
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