HEK293T (ATCC CRL-3216), U2OS (ATCC HTB-96), U2OS-265 (34 (link)) and U2OS DR-GFP (ATCC CRL-3455) cells were grown in DMEM (ThermoFisher Scientific 10569044) supplemented with 10% FBS (R&D solutions S11150) at 37°C with 5% CO2. U2OS-265 DSB reporter cells were a generous gift from Roger Greenberg (34 (link)).
U2os dr gfp
Manufactured by American Type Culture Collection
The U2OS DR-GFP is a cell line that has been genetically modified to express a green fluorescent protein (GFP) reporter for the detection of DNA double-strand break repair activity. The core function of this product is to provide a tool for the study of DNA damage response and repair mechanisms in human cells.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t, by American Type Culture Collection (1 mentions)
Nci h1341, by American Type Culture Collection (1 mentions)
Nci h211, by American Type Culture Collection (1 mentions)
Nci h841, by American Type Culture Collection (1 mentions)
Nci h1048, by American Type Culture Collection (1 mentions)
Nci h446, by American Type Culture Collection (1 mentions)
Nci h146, by American Type Culture Collection (1 mentions)
Nci h524, by American Type Culture Collection (1 mentions)
Dms114, by American Type Culture Collection (1 mentions)
Nci h889, by American Type Culture Collection (1 mentions)
2 protocols using u2os dr gfp
1
Culturing Various Cell Lines
2
SCLC Cell Lines and PDX Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NCI‐H211, NCI‐H524, DMS‐114, NCI‐H841, NCI‐H1048, NCI‐H1341, NCI‐H446, NCI‐H146, NCI‐H889, and U2OS DRGFP (Nakanishi et al, 2011 (link)) cell lines were purchased from ATCC. NCI‐H211, NCI‐H889, NCI‐H1048, NCI‐H1341, and U2OS DRGFP cell lines are female and the rest are male, additional information for cell lines can be observed in Table 1 . Cell lines were authenticated using short tandem repeat analysis, and were monthly tested for mycoplasma contamination. PDX‐03 and PDX‐06 were derived from a male and a female SCLC patient, respectively. Cell medium was RPMI‐1640 supplemented with 10% FBS for all lines to maintain consistency. DT40 (chicken cell lines) were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with FBS 10% and chicken serum 5%. Cells were grown at 37°C and 5% CO2.
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