Potassium phosphate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Potassium phosphate is a chemical compound that serves as a buffer solution, commonly used in various laboratory applications. It maintains a stable pH level in aqueous solutions, enabling controlled and consistent conditions for experiments and analyses.

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Lab products found in correlation

23 protocols using potassium phosphate

In Vitro Digestion Protocol

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Ethanol, sodium phosphate, potassium phosphate, acetonitrile, sodium hydroxide, trichloroacetic acid, hydrochloric acid, gallic acid, potassium ferricyanide, ferric chloride and calcium chloride dihydrate were purchased from Fisher Scientific (Pittsburgh, PA, USA). Folin–Ciocalteu’s reagent, potassium chloride, ammonium carbonate, acetic acid glacial and sodium chloride were purchased from Panreac Applichem (Barcelona, Spain). Magnesium chloride hexahydrate and aluminum chloride were from Acros Organics (Pittsburgh, PA, USA), and sodium carbonate anhydrous was purchased from Labkem (Barcelona, Spain).
Human salivary α-amylase (A1031-1KU), porcine pepsin (P6887), porcine pancreatin 4 × USP specifications (P1750), bile bovine (B3883), 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin, chrysin and p-coumaric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Kaempferol was purchased from Extrasynthese (Genay, France) and naringenin from Acros Organics (Pittsburgh, PA, USA).

Aylanc V., Tomás A., Russo-Almeida P., Falcão S.I, & Vilas-Boas M. (2021). Assessment of Bioactive Compounds under Simulated Gastrointestinal Digestion of Bee Pollen and Bee Bread: Bioaccessibility and Antioxidant Activity. Antioxidants, 10(5), 651.

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MycG4 DNA NMR Spectroscopy Protocol

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A 1.2 mM solution of MycG4 DNA was prepared with 10 mM potassium phosphate (Fisher Scientific) buffer, pH 7, 90% H₂O/10% D₂O. The NMR spectra were measured on a Bruker AV-800 MHz spectrometer equipped with a QCI cryoprobe at 25 °C using excitation sculpting for water suppression. All 2D experiments were performed at a berberine–MycG4 ratio of 3:1. The NOESY mixing times were between 80 ms and 300 ms, and additional NOESY experiments were done at 35 °C. ¹H–¹³C HSQ experiments was recorded optimized for a ¹J(C,H) of 190 Hz. Chemical shift referencing was done directly for ¹H based on the water signal relative to 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) and indirectly for ¹³C relative to DSS. The spectra were processed with Topspin 3.5 (Bruker) and analyzed with CcpNmr Analysis 2.4.³⁸

Dickerhoff J., Brundridge N., McLuckey S.A, & Yang D. (2021). Berberine Molecular Recognition of the Parallel MYC G-Quadruplex in Solution. Journal of medicinal chemistry, 64(21), 16205-16212.

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Spectroscopic Characterization of Heme Proteins

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Phenacetin, dilauroyl-l-3-phosphatidyl choline (DLPC), sodium dithionite, polyvinyl pyrolidone (PVP) and D₂O were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium phosphate and EDTA were purchased from Fisher Scientific (Pittsburgh, PA, USA). Emulgen^® and Chaps^® were obtained from the Chemical Division of the KAO Corporation (Tokyo, Japan) and EMD Biosciences (Lajolla, CA, USA), respectively. Deuterium oxide (D₂O, 99.9%) was obtained from Cambridge Isotope Laboratory (Andover, MA, USA). Centricon 10 kDa MW cut-off filters were purchased from Millipore Corporation (Billerica, MA, USA). Carbon monoxide was obtained from Mountain State AirGas (Morgantown, WV, USA). All other chemicals were of analytical grade and were purchased from standard commercial sources.

Huang Q, & Szklarz G.D. (2018). Increased Phenacetin Oxidation upon the L382V Substitution in Cytochrome P450 1A2 is Associated with Altered Substrate Binding Orientation. International Journal of Molecular Sciences, 19(6), 1580.

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Cryopreserved Hepatocyte Incubation Protocol

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Cryopreserved human hepatocytes, HLM, thawing and incubation media were
purchased from BioreclamationIVT (Baltimore, MD, USA). NADPH regenerating system
solutions A and B were purchased from Corning, Inc. (part #s 451220 and
451200, respectively). AH-7921 (97.94±1.04% purity) was kindly
provided by the Drug Enforcement Administration Special Testing and Research
Laboratory (Dulles, VA, USA). Liquid chromatography-mass spectrometry
(LC-MS)grade acetonitrile and Trypan Blue were purchased from Sigma-Aldrich (St.
Louis, MO, USA), while potassium phosphate, glacial acetic acid and formic acid
(LC-MS grade) were obtained from Fisher Scientific (Waltham, MA, USA). APCI
positive calibration solution was from SCIEX (part # 4460131, Redwood
City, CA, USA). Water was purified in house with an ELGA Purelab Ultra Analytic
purifier (Siemens Water Technologies, Lowell, MA, USA).

Wohlfarth A., Scheidweiler K.B., Pang S., Zhu M., Castaneto M., Kronstrand R, & Huestis M.A. (2015). Metabolic characterization of AH-7921, a synthetic opioid designer drug: in vitro metabolic stability assessment and metabolite identification, evaluation of in silico prediction, and in vivo confirmation. Drug testing and analysis, 8(8), 779-791.

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Iodometric Titration Reagents and Compounds

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Reagents for the iodometric titrations were purchased from Hach (dissolved oxygen three powder pillows, potassium iodide powder pillows, sodium thiosulfate digital titrator cartridge, 0.113 N, and starch indicator solution). Sodium chloride (NaCl), sodium thiosulfate (Na₂S₂O₃, STS), potassium chloride (KCl), p-cresol, sodium phosphate, and potassium phosphate were purchased from Fisher Scientific. Water from MilliQ water purification system (MilliporeSigma, Burlington, MA) was used for all experiments. HOCl (pH 4) was provided by Briotech Inc. (Woodinville, WA) as a commercially available end product; higher starting concentrations were used for spray studies, whereas lower starting concentrations were used for solution studies. High-pressure liquid chromatography (HPLC)–grade water and acetonitrile, butyric acid, DMDS, indole, and DMTS were purchased from Sigma Aldrich. Nutrient broth, bacto agar, and tryptic soy agar for Escherichia coli and MS2 culture are from BD Difco.

Dennler-Church T.E., Butz J.C., McKinley J.E., Keim E.K., Hall M.C., Meschke J.S., Mulligan J.M., Williams J.F, & Robins L.I. (2020). Modification of Major Contributors Responsible for Latrine Malodor on Exposure to Hypochlorous Acid: The Potential for Simultaneously Impacting Odor and Infection Hazards to Encourage Latrine Use. The American Journal of Tropical Medicine and Hygiene, 103(6), 2584-2590.

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Radioligand Binding Assay Protocol

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[³H]Nicotine (specific activity, 66.9 Ci/mmol) was purchased from PerkinElmer Life and Analytical Sciences (Boston, MA), while [³H]methyllycaconitine (specific activity, 100 Ci/mmol) and [³H]dofetilide (specific activity 80 Ci/mmol) were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO). (+)-Methamphetamine hydrochloride, sodium chloride, perchloric acid, disodium ethylenediamine tetra acetate (EDTA), ethyleneglycol tetra acetate (EGTA), L-(+) tartaric acid, N-[2-hydroxyethyl] piperazine-N’-[2-ethanesulfonic acid] (HEPES), dopamine hydrochloride, magnesium sulfate, D-glucose, 1-octanesulfonic acid sodium salt, amitriptyline, polyethyleneimine, ascorbic acid, potassium aspartate, adenosine triphosphate, and ascorbate oxidase were purchased from Sigma-Aldrich (St. Louis, MO). Citric acid, sodium bicarbonate, sodium phosphate, potassium chloride, calcium chloride, potassium phosphate and magnesium chloride were purchased from Fisher Scientific Co. (Pittsburgh, PA).

Nickell J.R., Siripurapu K.B., Horton D.B., Zheng G., Crooks P.A, & Dwoskin L.P. (2016). GZ-793A inhibits the neurochemical effects of methamphetamine via a selective interaction with the vesicular monoamine transporter-2. European journal of pharmacology, 795, 143-149.

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Analytical Grade Reagent Protocol

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All chemicals and biochemicals were of analytical (or higher) grade. Potassium phosphate was purchased from Fisher (Waltham, MA USA), NAD⁺ was from Calbiochem (Billerica, MA USA); all other reagents were from Sigma Chemical Co (St. Louis MO USA).

Vernau K., Napoli E., Wong S., Ross‐Inta C., Cameron J., Bannasch D., Bollen A., Dickinson P, & Giulivi C. (2014). Thiamine Deficiency‐Mediated Brain Mitochondrial Pathology in Alaskan Huskies with Mutation in SLC19A3.1. Brain Pathology, 25(4), 441-453.

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HPLC-Based Compound Quantification

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HPLC-grade solvents, ascorbic acid, DTPA, methylmonoarginine, o-phthalaldehyde, PCA, potassium hydroxide, and potassium phosphate were purchased from Fisher Scientific (Pittsburgh, PA, USA).

Ballard K.D., Mah E., Guo Y., Bruno R.S., Taylor B.A., Beam J.E., Polk D.M, & Thompson P.D. (2016). Single Low-Density Lipoprotein Apheresis Does Not Improve Vascular Endothelial Function in Chronically Treated Hypercholesterolemic Patients. International Journal of Vascular Medicine, 2016, 4613202.

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Bacillus subtilis Biofilm Cultivation Protocol

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Bacillus subtilis NCIB 3610 amyE::P_tapA-Ypet strain (ES2107) was used for all experiments. The strain was cultured on lysogeny broth (LB)-agar plates prepared from Miller LB (Acros Organics, Fair Lawn, NJ) and 1.5% agar powder (Molecular Genetics, Fisher Scientific, Waltham, MA). LB agar plates were streaked with B. subtilis ES2107 from frozen stocks and incubated at 30°C for 15 h.
B. subtilis biofilms were grown on MSgg, a B. subtilis biofilm-promoting medium (5 mM potassium phosphate [pH 7]) (Fisher Scientific, Waltham, MA), 100 mM MOPS (morpholinepropanesulfonic acid [pH 7]) (Sigma-Aldrich, St. Louis, MO), 2 mM MgCl₂ (Fisher Scientific, Waltham, MA), 700 μM CaCl₂ (Alfa Aesar, Haverhill, MA), 50 μM MnCl₂ (Fisher Scientific, Waltham, MA), 50 μM FeCl₃ (Sigma-Aldrich, St. Louis, MO), 1 μM ZnCl₂ (Sigma-Aldrich, St. Louis, MO), 2 μM thiamine hydrochloride (Fisher Scientific, Waltham, MA), 0.5% glycerol (Fisher Scientific, Waltham, MA), and 0.5% glutamate (Sigma-Aldrich, St. Louis, MO). For MSgg agar plates, 1.5% agar (BD) was added to the medium, and approximately 5-ml plates were poured.

Lukowski J.K., Bhattacharjee A., Yannarell S.M., Schwarz K., Shor L.M., Shank E.A, & Anderton C.R. (2021). Expanding Molecular Coverage in Mass Spectrometry Imaging of Microbial Systems Using Metal-Assisted Laser Desorption/Ionization. Microbiology Spectrum, 9(1), 10.1128/spectrum.00520-21.

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Synthesis of Organic Compounds

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Example 1

Phenylmagnesium bromide (PMB) was purchased from Sigma-Aldrich at a 3M concentration in diethyl ether. Carbon disulfide was purchased from Sigma-Aldrich at 99% purity. 3-Chloro-2-butanone was purchased from Sigma-Aldrich at 97% purity. Potassium phosphate was purchased from Fisher Scientific at 97% purity. HPLC grade acetone was purchased from Fisher Scientific. Ethanethiol was purchased from Sigma-Aldrich at 97% purity. Styrene and n-butyl acrylate were purchased from Sigma-Aldrich at 99% purity. Initiators azobisisobutyronitrile (AIBN) and di-tert-butyl peroxide (TBP) were all purchased from Sigma Aldrich.

US10968173B2. Thiocarbonylthio compounds as chain transfer agents suitable for raft polymerization (2021-04-06). IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. [US]. Inventors: Eric W. Cochran [US], Nacu Hernandez [US], Michael Forrester [US], Austin Hohmann [US].

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