A300 spectrometer x band

Electron paramagnetic resonance (EPR) spectra of H64D Mb (0.3 mM, in 100 mM KPi buffer, pH 6.0) and A15C/H64D Ngb (0.3 mM, in 100 mM KPi buffer, pH 7.0) in the absence or presence of MG (0.3 mM) were recorded on a Bruker A300 spectrometer (X-band) equipped with Bruker ER4141VTM liquid nitrogen system. The sample was transferred into an EPR tube with a volume of 300 μL. The spectrum was measured at 100 K, with a frequency of 9.27 GHz, centerfield 2750 G and sweep width 4500 G, microwave power 0.595 mW, and modulation amplitude 3.0 G.

Electron paramagnetic resonance (EPR) spectra of F43H/H64A Mb (0.5 mM, in 100 mM KPi buffer, pH 6.0) in the absence or presence of MG (1 mM) were recorded on a Bruker A300 spectrometer (X-band) equipped with Bruker ER4141VTM liquid nitrogen system. The sample was transferred into an EPR tube with a volume of 300 μL. The spectrum was measured at 100 K, with frequency of 9.43 GHz, center field 2200 G and sweep width 3600 G, microwave power 0.595 mW and modulation amplitude 3.0 G.

TEM imaging was performed on a JEM-2100 (JEOL, Japan) transmission electron microscope at an acceleration voltage of 200 kV. Powder XRD patterns were measured at 5°/min using a diffractometer (Rigaku Ultima III, Japan and Bruker D8 advance, Germany) with a Cu Kα radiation. XPS spectra were collected by using a PHI 5000 Versa Probe XPS microscope (Ulvac-Phi, Japan). Ultraviolet-visible absorption spectra were measured on a SpectraMax M2e microplate reader (Molecular Devices, USA). HAADF-STEM and the corresponding energy-dispersive spectroscopy elemental mappings were performed on a Tecnai G2 F30 (FEI, USA). EPR spectra were recorded on a Bruker A300 spectrometer (X-band). The pH value of MRS medium was measured using a pH meter (STARTER 3100, USA). Cell images of C. albicans and Lactobacillus were recorded on a laser scanning confocal microscope (Olympus, Japan). Tissue images of H&E staining were photographed by a DMi8 fluorescence microscope (Leica, Germany).