FTO-patterned glass substrates were purchased from Asahi glass (Tokyo, Japan). Lead iodide (PbI2), methylammonium bromide (MABr), lead bromide (PbBr2), formamidinium iodide (FAI), and cesium iodide (CsI) were purchased from Tokyo Chemical Industry (Tokyo, Japan). The N,N-dimethylformamide (DMF; 99.5% purity) and dimethyl sulfoxide (DMSO; 99.5% purity) were purchased from Wako Pure Chemical (Osaka, Japan). Titanium diisopropoxide bis(acetylacetonate) (75 wt% in isopropanol) and 2-isopropanol (99.9% purity) were supplied from Sigma-Aldrich, Wako Pure Chemical, respectively.
2 isopropanol
Manufactured by Merck Group
Sourced in Germany
2-Isopropanol is a colorless, flammable liquid chemical compound. It is commonly used as a solvent and disinfectant in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Milli q water purification system, by Merck Group (2 mentions)
Rifapentine reference material, by Sanofi (2 mentions)
25 o desacetyl rifapentine reference material, by LGC (2 mentions)
Rifampicin d3, by LGC (2 mentions)
Lc ms ms grade millipore water, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Acetonitrile, by Honeywell (2 mentions)
Cesium iodide, by Tokyo Chemical Industry (1 mentions)
N n dimethylformamide (dmf), by Fujifilm (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Formamidinium iodide, by Tokyo Chemical Industry (1 mentions)
Titanium diisopropoxide bis acetylacetonate, by Merck Group (1 mentions)
Agar powder, by Merck Group (1 mentions)
Aceton, by Merck Group (1 mentions)
Trichloracetic acid, by Merck Group (1 mentions)
Catalase, by Merck Group (1 mentions)
Potassium hexachloroplatinate 4, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
11 protocols using 2 isopropanol
1
Perovskite Thin-Film Fabrication
2
Spectrophotometric Assay of Oxidative Enzymes
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Dimethylformamide (DMF), trichloracetic acid (TCA), horseradish peroxidase (HRP) (type VI, ≥250 units/mg), catalase (from bovine liver, 2,000–5,000 units/mg), 4-aminoantipyrine (4-AP), phenol, graphene oxide (GO), activated charcoal, agar powder, potassium chloride, silver nitrate, potassium hexachloroplatinate (IV), gold(III) chloride trihydrate, aceton, 2-isopropanol, ethanol, and other chemicals were purchased from Sigma-Aldrich (St. Louis, United States). All reagents were of analytical grade. Mn(III) meso-tetra(N-methyl-4-pyridyl) porphine pentachloride (MnTMPyP) and Mn(III) meso-tetra(4-pyridyl) porphine chloride (MnTPyP) (>95%) were obtained from Frontiers Scientific Inc. Phosphate buffer (PB) solutions (0.1 M, pH 7.4) were prepared from disodium hydrogen phosphate and sodium dihydrogen phosphate. The pH of the solutions was controlled by the pH-meter 765 (Knick GmbH). All solutions were prepared using a Milli-Q water purification system (Merk KGaA, Germany).
3
Efficient Isolation of MONO Fraction
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Example 7
To further improve the method for the isolation of a MONO fraction from mixtures of ZERO, MONO and POLY, liquid chromatography was conducted with various combinations of stationary and mobile phases. Excellent results in terms of purity and yield were obtained with dried silica high-purity grade, pore size 60 Å, 230-400 mesh particle size, 40-63 μm (Fluka, catalogue number 60737) and an eluent consisting of 12 v/v % triethylamine (Sigma-Aldrich, catalogue number 90340). 12 v/v % 2-isopropanol (Sigma-Aldrich, catalogue number 24137; Ph. Eur) and 76 v/v % heptane (Sigma-Aldrich, catalogue number 32287; Ph. Eur). Twenty g of M10 in 60 mL eluent were applied to a column prepared from 260 g silica (dried in a vacuum oven for at least 3 h at 160° C. and <100 mBar) in about 500 mL of eluent. The column was eluted at a flow of about 3 L per h and fractions of 50 mL were collected. Each fraction was analyzed by HPTLC (Sigma-Aldrich Uniplate Catalogue number Z26531-4) with 20 v/v % isopropanol in heptane as eluent. M10-ZERO was eluted at between 400 and 600 mL. M10-MONO was eluted at between 800 and 3200 mL. POLY remained on the column and could be eluted with 100% 2-isopropanol. A single liquid chromatography run resulted in a recovery of >90% M10-MONO with <2% ZERO and <2% POLY as determined by semi-quantitative HPTLC analysis.
4
Perovskite Solar Cell Material Synthesis
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Lead (II) iodide PbI2 ultradry 99.999% (metals basis) was purchased from Alfa Aesar, Formamidinium iodide HC(NH2)2I (FAI) from Xi’an Polymer Light Technology Corp. Corn starch (Maizena) was purchased from Unilever, DMSO was purchased from Fisher BioReagents. 2-isopropanol (HPLC, 99.9%), ethanol (anhydrous, 99.8%), Polyethylenimine (PEIE) 80% ethoxylated solution, Zinc acetate dihydrate (99.999%), were all purchased from Sigma Aldrich.
Poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,4′-(N-(4-sec-butylphenyl)diphenylamine)] (TFB) was supplied from American Dye source Inc. M-xylene 99% extra pure was purchased from Acros Organics. All the materials were used as received without any further purification. ZnO nanocrystals was prepared as previous reported33 (link). ITO substrates were purchased from Shenzhen Huayu Union Technology Co. Ltd.
Poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,4′-(N-(4-sec-butylphenyl)diphenylamine)] (TFB) was supplied from American Dye source Inc. M-xylene 99% extra pure was purchased from Acros Organics. All the materials were used as received without any further purification. ZnO nanocrystals was prepared as previous reported33 (link). ITO substrates were purchased from Shenzhen Huayu Union Technology Co. Ltd.
5
Analytical Standards for Chloramphenicol Assay
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Analytical standards of chloramphenicol (CAP, CAS 56-75-7), 2-isopropanol, acetic acid, deuterated internal standard (CAP-d5), dimethyl sulfoxide (DMSO), fetal bovine serum (FBS), bovine calf serum (BCS), bovine serum albumin, 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT), neutral red dye, Triton X-100, trypsin-EDTA, collagenase type IV, insulin, hydrocortisone, and antibiotic solutions (penicillin and streptomycin) were purchased from Sigma-Aldrich, Poznań, Poland. Chloramphenicol glucuronide (CAP-G, CAS 39751-33-2) was obtained from Toronto Research Chemicals (Toronto, ON, Canada). All other chemicals were sourced from commercial suppliers and were of the highest available purity.
6
Cardiac miRNA Expression Analysis via Trizol-Chloroform RNA Extraction
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The hearts were homogenized with 1 ml Trizol (Invitrogen) for 50 mg of tissue. Then were added 0.2 ml chloroform per each ml of the used Trizol. The samples have been put in shaking for 15 s, left in ice and centrifuged with 13,000 rpm for 20 min at 4 °C. The supernatant with RNA was transferred in a new eppendorf. RNA was then precipitated with 0.5 ml of isopropanol (2-isopropanol, Sigma) per each ml of Trizol used. The samples were incubated for a night at −20 °C and, after that, centrifuged at 13,000 rpm for 20 min at 4 °C; the new pellet was re-suspended in 1 ml ethanol/75% DEPC and centrifuged. The pellet was than dried at room temperature before being re-suspended in water with diethyl-pyrocarbonate (DEPC) and stored at −80 °C. Total RNA was analysed by Pico-drop (Perkin Elmer), electrophoresis and used for RT-PCR and qRT-PCR to evaluate the relative expression of cardiac miRs.
7
Lipid Extraction from Cell Cultures
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To evaluate lipid production cells were collected at different growing times by centrifugation at 3,100 g for 10 minutes. Lipids were extracted using an adapted solvent-based method described by Hara et al. [59 (link)]. A solution of hexane / 2-isopropanol (3:2, v/v) (both purchased from Sigma-Aldrich, St. Louis, MO) was added to every cell pellet previously thawed at room temperature. After vigorously vortexing, tubes were left for 3 hours with shaking. Then they were centrifuged for 7 min at 12,000 g and 8°C, the upper organic phase was collected and placed into new 2 mL tube and the solvent was evaporated in a rotational vacuum concentrator (Christ RVC2-18, Germany) at 40°C.
8
HA-Based Soft Tissue Filler Characterization
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HA (average mw 500 kDa) was purchased from Bloomage Freda Biopharm Co., Ltd. PEG methyl ether (PEGME, mw 5 kDa), PVP (mw 10 kDa), Sorbitan monooleate (Span® 80), Rhodamine 6G, Hoechst 33258, 2-isopropanol, acetone, penicillin-streptomycin and light mineral oil were purchased from Sigma-Aldrich. Divinyl sulfone (DVS) was purchased from VWR. The Dulbecco's Modified Eagle Medium (high glucose, L-Glut, phenol red) and the foetal bovine serum were purchased from Life Technologies. The AlamarBlue reagent was purchased from invitrogen TM . Two millilitres of commercialized HA-based soft tissue fillers (BioHyalu, Freda Biopharm) was kindly provided by the Plastic Surgery Department, Nanfang Hospital, Guangzhou, China.
9
Quantitative Analysis of Rifapentine and Metabolites
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Rifapentine reference material was donated by Sanofi (Bridgewater, New Jersey, United States) while 25-O-desacetyl Rifapentine reference material and rifampicin-d3 were purchased from Toronto Research Chemicals (North York, Ontario, Canada). Methanol and ascorbic acid were obtained from Sigma-Aldrich (Darmstadt, Germany), while 2-iso-propanol and formic acid were purchased from Merck (Darmstadt, Germany). Acetonitrile was purchased from Honeywell (Pittsburgh, USA). LC-MS/MS grade Millipore water was sourced in-house (Merck-Millipore, Germany).
10
Enzymatic Assay for Oxidative Stress
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Bovine liver catalase (H2O2:H2O2 oxidoreductase EC 1.11.1.6, 2000–5000 units mg−1), xanthine oxidase (XO) from bovine milk (xanthine:oxygen oxidoreductase, EC 1.17.3.2, ≥0.4 units mg−1), peroxidase from horseradish (type VI, 310 units mg−1) (HRP), cytochrome c from equine heart (λmaxreduced form = 550 nm), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), superoxide dismutase (1854 units mg−1) (SOD), acetone, 2-isopropanol, manganese(II) chloride tetrahydrate, and other analytical grade chemicals were purchased from Merck KGaA, Darmstadt, Deutschland. Phosphate buffers were prepared from sodium dihydrogen phosphate and disodium hydrogen phosphate. pH 2.0 and pH 3.0 buffers were prepared from phosphoric acid and sodium dihydrogen phosphate. The pH of the solutions was measured using a pH meter (Knick GmbH, Berlin, Germany). The O2 concentration was monitored using an oxygen sensor (LE621-IP67, MettlerToledo GmbH, Gießen, Germany).
All solutions were prepared using a Milli-Q water purification system (Merk KGaA, Darmstadt, Germany). Before the experiments began, the value of the extinction coefficient, ε340, of the ABTS product was examined, and it corresponded to 3.7 × 104 M−1 cm−1 [43 (link)].
All solutions were prepared using a Milli-Q water purification system (Merk KGaA, Darmstadt, Germany). Before the experiments began, the value of the extinction coefficient, ε340, of the ABTS product was examined, and it corresponded to 3.7 × 104 M−1 cm−1 [43 (link)].
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