After approval by the institutional ethics committee of Saint Joseph University, Beirut, Lebanon (USJ-2017-55), 85 lower premolars, extracted for reasons unrelated to the study, were cleaned using an ultrasonic insert (1S, Satelec Acteon Group, Mérignac, France) and stored in 0.1% formocresol. Teeth were inspected under an operating microscope (Zeiss
Extaro 300, Oberkochen, Germany) at x25 magnification to eliminate teeth with cracks or advanced external resorption. Mesiodistal and vestibulolingual X-rays were taken (Sopix, Satelec Acteon Group, Merignac, France) to discard teeth with treated canals, pulpal calcification, or internal resorption. Cone beam computed tomography (Newtom VGI, Verona, Italy) (CBCT) was performed, and only teeth with mature apices and a single oval canal with a moderate curvature of 15 to 22 degrees according to the Schneider technique, were included in the study [18 (
link),19 (
link),20 (
link)]. Finally, 30 mandibular premolars were selected. Access cavity was prepared using an 856 diamond bur (Komet Italia SRL, Milan, Italy) with a high-speed handpiece under running water under an operating microscope, and a
size #10 k-file (Dentsply Sirona, Ballaigues, Switzerland) was introduced to verify patency. This study followed the CRIS guidelines for in vitro studies, as discussed in the 2014 concept note [21 (
link)].
Kaloustian M.K., Hachem C.E., Zogheib C., Nehme W., Hardan L., Rached P., Kharouf N., Haikel Y, & Mancino D. (2022). Effectiveness of the REvision System and Sonic Irrigation in the Removal of Root Canal Filling Material from Oval Canals: An In Vitro Study. Bioengineering, 9(6), 260.