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41 protocols using size 10 k file

1

Standardized Single-Canal Incisor Preparation

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Sixty-eight human permanent mandibular incisors with a single canal and similar root lengths, were collected. Calculus on the external root surface and soft tissue remnants was mechanically removed. The samples were disinfected in 0.5% chloramine T, stored in distilled water at 4°C and used within 6 months of extraction. Digital radiographs were taken to determine the angle of curvature, using an image analysis program (AxioVision 4.5; Carl Zeiss Vision, Hallbergmoos, Germany) (19) . Only roots with the angle of curvature <10° and an initial apical size equivalent to a size-10 K-file (Dentsply Maillefer, Baillagues, Switzerland) were selected for the study (n = 40).
Straight-line access cavities were made and apical patency was determined by inserting a size-10 K-file into the root canal until its tip was visible at the apical foramen. The working length (WL) was 1 mm smaller than this measurement. Then, the foramen diameters of all specimens were standardized to a size-15 K-file (Dentsply Maillefer). The specimens were sterilized with ethylene oxide gas for 12 h using an Anprolene system at 74°C (Andersen Products Inc.; Haw River, NC, USA).
This study was approved by the Ethical Committee of Grande Rio University (#47448315.2.0000.5283).
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2

Root Canal Instrumentation and Irrigation

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The tooth crowns were removed at the cementoenamel junction level by using a 0.3 mm thick diamond disk fixed in an Isomet saw (Isomet, Buehler, Lake Bluff, Illinois, USA). The apical patency was determined by inserting a size 10 K-file (Dentsply Maillefer, Ballaigues, Switzerland) until it reached the apical foramen. Then working length (WL) was established at 1 mm short of the apical foramen.
A single operator prepared all the root canals using the Mtwo rotary instrumentation system (VDW, Munich, Germany) until a 35.04 diameter was obtained. Root canals were irrigated with 2.5 mL of 2.5% sodium hypochlorite (NaOCl) after each instrument by using a disposable syringe and a 27-G NaviTip needle (Ultradent, South Jordan, UT). On conclusion of root canal instrumentation, three applications of 2.5 mL of 2.5% NaOCl and 17% EDTA were made by means of passive ultrasonic activation (PUI) for 20 s each, to achieve improved isthmus cleaning24. Then, the root canals were finally irrigated with 5 mL of saline solution and dried with paper points.
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3

Standardized Root Canal Preparation

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Sixty extracted human single-root premolars were selected after being radiographed buccolingually and mesiodistally. Inclusion criteria were as follows: single straight root canal, completely formed apex with patent foramina, no obstruction within canal system, and no evidence of internal and/or external resorption. Selected teeth were decoronated at 16 mm from the apex to standardize the length of all specimens. After pulp extirpation, size 10 K-file (Dentsply Maillefer) was introduced into the canal until it was visible at the apical foramen. True working length was established by subtracting 1.0 mm from this measurement. Root canal preparation was carried out using a modified step-down technique 22) . Coronal two-third was flared using Gates-Glidden drills sizes 4, 3, and 2 (Dentsply Maillefer). Apical third was prepared using K-files up to master apical file size 35, followed by stepback in 1-mm increments up to file size 60. Canals were irrigated between each file change with 3 mL of 5.25% NaOCl (Clorox (M) Industries, Malaysia). Final irrigation was done using 5 mL of 17% EDTA (SmearClear™, SybronEndo, Orange, CA, USA) for 3 min to eliminate the smear layer, followed by irrigation with 5 mL of distilled water and drying with paper points (Dentsply Maillefer).
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4

Preparation and Mounting of Human Molars

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Thirty-six sound-extracted human second primary mandibular molars, with no physiological root resorption, were collected and stored in distilled water. Teeth with pathological resorption, fractures, severe curve (a root canal curvature > 20° [22 (link)]), perforation in the furcation area, and less than two thirds of the root were excluded from the study.
Access cavities were prepared using 330 high-speed carbide bur (Mailleffer, Ballaigues, Switzerland) with water spray. A size 10 K-file (Dentsply Maillefer, Tulsa, OK, USA) was placed into the canals until it was visible at the apical foramen, and the working length was established as 1 mm short of a length. All specimens were mounted vertically in auto-polymerizing acrylic resin (UNIFAST Trad, Gc America).
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5

Root Canal Preparation Standardization

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The crowns were removed at the cementoenamel junction using a high-speed handpiece and bur under copious water spray, standardizing the length of the remaining root to 9 mm. The coronal canal enlargement was performed using Gates Glidden bur #2 and #3 (Dentsply Sirona, Ballaigues, Switzerland). A size 10 K-file (Dentsply Sirona, Ballaigues, Switzerland) was inserted into the canal until the tip of the file was visible at the apical foramen. The working length was determined by subtracting 1 mm from this length and confirmed from the radiographs taken. All root canals were instrumented with Profile Ni-Ti rotary files (Dentsply Maillefer, Ballaigues, Switzerland) until the #40/06 file reached the working length. Between each instrumentation step, patency was maintained by passing a size 10 K-file to the apical foramen. Each canal was irrigated with 1 mL of 6% NaOCl solution using a 30-gauge needle. Upon the completion of instrumentation, the canal was rinsed with 1 mL of 17% ethylenediaminetetraacetic acid (EDTA) for 1 min to remove the smear layer, followed by 5 mL of 6% NaOCl. All canals were dried with absorbent paper points (Millimeter-Marked Paper Points, Diadent, Cheongju, Korea).
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6

Standardized Root Canal Preparation

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Thirty human teeth with single, straight root canals that were extracted for periodontal, orthodontic, or prosthetic reasons were selected for this study. All the extracted teeth were numbered and kept in 5.25% sodium hypochlorite (NaOCl) for 2 h and then stored in sterile 0.9% saline solution. The teeth were decoronated at the cementoenamel junction with a diamond disc to simplify access to the root canal and to obtain a reliable occlusal landmark. Gates-Glidden drills (Dentsply Maillefer, Ballaigues, Switzerland) #3 and #4 were used to flare the coronal one-thirds of each root canal. A 2.5% NaOCl solution was used for irrigation during the process and the patency of the apical foramen was maintained using size 10 K-File (Dentsply, Maillefer, Switzerland).
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7

Selection of Premolars for Endodontic Research

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After approval by the institutional ethics committee of Saint Joseph University, Beirut, Lebanon (USJ-2017-55), 85 lower premolars, extracted for reasons unrelated to the study, were cleaned using an ultrasonic insert (1S, Satelec Acteon Group, Mérignac, France) and stored in 0.1% formocresol. Teeth were inspected under an operating microscope (Zeiss Extaro 300, Oberkochen, Germany) at x25 magnification to eliminate teeth with cracks or advanced external resorption. Mesiodistal and vestibulolingual X-rays were taken (Sopix, Satelec Acteon Group, Merignac, France) to discard teeth with treated canals, pulpal calcification, or internal resorption. Cone beam computed tomography (Newtom VGI, Verona, Italy) (CBCT) was performed, and only teeth with mature apices and a single oval canal with a moderate curvature of 15 to 22 degrees according to the Schneider technique, were included in the study [18 (link),19 (link),20 (link)]. Finally, 30 mandibular premolars were selected. Access cavity was prepared using an 856 diamond bur (Komet Italia SRL, Milan, Italy) with a high-speed handpiece under running water under an operating microscope, and a size #10 k-file (Dentsply Sirona, Ballaigues, Switzerland) was introduced to verify patency. This study followed the CRIS guidelines for in vitro studies, as discussed in the 2014 concept note [21 (link)].
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8

Mandibular Molar Root Canal Instrumentation

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Approval for this study was obtained from the local IRB (protocol no. 1.472.852). The mesiobuccal canals of 96 extracted mandibular molars with curvatures of 10-20 degrees were used in this study according to the method described by Schneider (18) . The teeth were stored in a 0.9% saline solution until use. Pre-operative radiographs were taken to ensure that the teeth had no fractures, canal bifurcation, calcifications, or prior root canal treatment. The roots were sectioned transversely at the coronal third using a water-cooled diamond disk to obtain root specimens with a length of 15 mm. Apical patency was determined by inserting a size-08 K-file (Dentsply Maillefer, Ballaigues, Switzerland) into the root canal until the tip was visible at the apical foramen. Only narrow canals with initial apical diameters no wider than a size-10 K-file (Dentsply Maillefer) were included. All specimens were instrumented with #10 and #15 files calibrated at 15 mm under irrigation with distilled water. The root canals were filled with 17% EDTA for 3 min to remove the smear layer and washed with 5 mL of distilled water.
The apical foramen was then sealed with resin composite (Z250; 3M ESPE, St. Paul, MN, USA), and the root surfaces were covered with two layers of a nail varnish. Then, all specimens were autoclaved for 30 min at 120 °C, and further contamination was performed as described below.
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9

Single-rooted Anterior Tooth Preparation

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This study was approved by the University Ethics Committee (Protocol: 27745514.0.0000.5259). Fifty single-rooted anterior teeth with single canal and straight roots were selected for this study. The presence of immature root, root damage, calcifications, atresic root canals, or root length shorter than 13 mm were established as the exclusion criteria. Using a diamond disc, the selected teeth were sectioned at 12.5 mm from the anatomic apex, and two longitudinal grooves were prepared in the mesial and distal regions of roots to facilitate later splitting of the samples. After this, patency of all specimens was confirmed with a size 10 K file (Dentsply Maillefer, Rio de Janeiro, RJ, Brazil) and the apical foramen was standardized with a size 25 K file (Dentsply Maillefer).
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10

Endodontic Treatment: Cleaning and Shaping

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Access to the root canal was gained using a bur, and a size 10 K-file (Dentsply Sirona) was then used to establish a 15-mm working length (1 mm short of the apical foramen). A #10 K-file was used to extend the working length 1 mm beyond the anatomical apex to verify canal patency. Canals were cleaned and shaped with ProTaper NEXT NiTi rotary files J-STAGE Advance Publication: June 4, 2022 Journal of Oral Science (Dentsply Sirona) using five instruments, X1 to X5 (#50/variable taper, a 6% taper with a 3 mm tip), successively with a ProMark torque-limited electric motor (Dentsply Sirona). Canals were irrigated at each instrument change with 2 mL of 2.5% sodium hypochlorite (NaOCl) through a 27-G needle (Ultradent Inc., South Jordan, UT, USA). After irrigation with 5 mL of 17% EDTA for one minute, followed by 5 mL of 2.5% NaOCl, the canals were dried with ProTaper NEXT absorbent points (Dentsply Sirona).
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