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Fin56

Manufactured by Cayman Chemical
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FIN56 is a laboratory reagent produced by Cayman Chemical. It is a small molecule that functions as a selective inhibitor of the enzyme FIN56. The core function of FIN56 is to modulate the activity of this target enzyme in in vitro research applications.

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Lab products found in correlation

Ml210, by Cayman Chemical (2 mentions) Ferrostatin 1, by Cayman Chemical (1 mentions) Z vad fmk, by Cayman Chemical (1 mentions) N acetyl cysteine (nac), by Thermo Fisher Scientific (1 mentions) Erastin, by Cayman Chemical (1 mentions) Lovastatin, by Selleck Chemicals (1 mentions) Erastin, by Bio-Techne (1 mentions) Verdinexor, by Cayman Chemical (1 mentions) Tipifarnib, by MedChemExpress (1 mentions) Methotrexate, by Selleck Chemicals (1 mentions) Brequinar, by Selleck Chemicals (1 mentions) Alisertib, by Selleck Chemicals (1 mentions) Leptomycin b, by Cayman Chemical (1 mentions) Pitstop 2, by Merck Group (1 mentions) Dorsomorphin, by Selleck Chemicals (1 mentions) Erastin, by Merck Group (1 mentions) Nigericin, by Thermo Fisher Scientific (1 mentions) Staurosporine, by Cayman Chemical (1 mentions) Deferoxamine mesylate salt dfo, by Merck Group (1 mentions) Nec 1, by Enzo Life Sciences (1 mentions)

4 protocols using fin56

1

Generation and Characterization of ATF3 Knockout Cells

Cited 1 time
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HT1080 cells expressing ATF3 and empty vector were generated previously [26 (link)]. Retinal pigment epithelial (RPE) cells immortalized with hTERT were obtained from Dr. Todd Waldman [27 (link)]. To generate ATF3-knockout cells, a target sequence (5′-AAAATGATGCTTCAACACCC-3′) spanning the ATF3 start codon was inserted into pSpCas9(BB)-2A-puro, and used to transfect RPE and U2OS cells as described [28 (link)]. ATF3-knockout HCT116 cells were generated using rAAV-mediated homologous recombination as described previously [14 ]. These cells were cultured in media supplemented with 10% fetal bovine serum. We used DMEM for HT1080, RPE, DU145, and U2OS cells, RPMI 1640 for PC3 cells, and McCoy’s 5A for HCT116 cells. Erastin, RSL3, ferrostatin-1, BSO, Z-VAD-FMK, and FIN56 were purchased from Cayman Chemical. N-acetylcysteine (NAC) was purchased from Thermo Fisher Scientific. Other reagents were obtained from Sigma-Aldrich.
Wang L., Liu Y., Du T., Yang H., Lei L., Guo M., Ding H.F., Zhang J., Wang H., Chen X, & Yan C. (2019). ATF3 promotes erastin-induced ferroptosis by suppressing system Xc–. Cell Death and Differentiation, 27(2), 662-675.
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2

Ferroptosis Inducers and Inhibitors Screening

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erastin (Bio-techne, 5449); SAS (MedchemExpress, HY-14655); RSL3 (Cayman, 19288); FIN56 (Cayman, 25180); FINO2 (MedchemExpress, HY-129457); PANKi (Cayman, 31002); BSO (Sigma, B2515); DEM (Sigma, D97703); etomoxir sodium salt (Selleckchem, S8244); lovastatin (Selleckchem, S2061); TOFA (Selleckchem, S6690); dorsomorphin (Selleckchem, S7306); alisertib (Selleckchem, S1133); verdinexor (Cayman, 26171); leptomycin B (Cayman, 10004976); tipifarnib (MedchemExpress, HY-10502); methotrexate (Selleckchem, S1210); pitstop2 (Sigma, SML1169); EML425 (Selleckchem, S2977); C646 (Selleckchem, S7152); brequinar (Selleckchem, S3565); elamipretide (Selleckchem, S9803)
Chen S.Y., Lin C.C., Wu J., Chen Y., Wang Y.E., Setayeshpour Y., Mestre A, & Chi J.T. (2024). NINJ1 regulates ferroptosis via xCT antiporter interaction and CoA modulation. bioRxiv.
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3

Ferroptosis Regulators Protocol

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Lip-1 (Selleckchem, cat. no. S7699), doxycycline (Dox; Sigma, cat. no. D9891), RSL3 (Cayman, cat. no. 19288), BSO (Sigma, cat. no. B2515), iFSP1 (ChemDiv, cat. no. 8009-2626), icFSP1 (ChemDiv, cat. no. L892-0224 or custom synthesis by Intonation Research Laboratories), erastin (Merck, cat. no. 329600), ML210 (Cayman, cat. no. Cay23282-1), FIN56 (Cayman, cat. no. Cay25180-5), FINO2 (Cayman, cat. no. Cay25096-1), deferoxamine mesylate salt (DFO; Sigma, cat. no. 138-14-7), ferrostatin-1 (Fer-1; Sigma, cat. no. SML0583), zVAD-FMK (zVAD; Enzo Life Sciences, cat. no. ALX-260-02), necrostatin-1s (Nec-1s; Enzo Life Sciences, cat. no. BV-2263-5), MCC950 (Sigma, cat. no. 5381200001), olaparib (Selleckchem, cat. no. S1060), staurosporine (STS; Cayman, cat. no. 81590), recombinant human tumour necrosis factor (TNF; R&D Systems, cat. no. NBP2-35076), Smac mimic (BV-6; Selleckchem, cat. no. S7597), nigericin (Thermo Fisher, cat. no. N1495), IMP-1088 (Cayman, cat. no. Cay25366-1) and lipopolysaccharide (LPS; Sigma, cat. no. L2880) were used in this study.
Nakamura T., Hipp C., Santos Dias Mourão A., Borggräfe J., Aldrovandi M., Henkelmann B., Wanninger J., Mishima E., Lytton E., Emler D., Proneth B., Sattler M, & Conrad M. (2023). Phase separation of FSP1 promotes ferroptosis. Nature, 619(7969), 371-377.
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4

Ferroptosis Induction and Inhibition Assay

Cited 1 time
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All cell lines used in this study were purchased from the American Type Culture Collection and were free of mycoplasma contamination (tested by the vendor). No cell line used in this study has been found in the International Cell Line Authentication Committee database of commonly misidentified cell lines, based on short tandem-repeat profiling performed by the vendor. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (vol/vol) fetal bovine serum (FBS) and 1% (vol/vol) penicillin/streptomycin in a humidified atmosphere of 5% CO2 at 37 °C as described previously (38 (link), 39 (link)). All cell lines were seeded into a 12-well plate for cell death and lipid peroxidation measurements. Cells were treated with ferroptosis inducers, including RSL3 (Selleck Chemicals), ML210 (Cayman Chemical), ML162 (Cayman Chemical), and FIN56 (Cayman Chemical); ferroptosis inhibitors, including liproxstatin-1 (Cayman Chemical) and ferrostatin-1 (Sigma-Aldrich); deferoxamine (Sigma-Aldrich); glycerol (Sigma-Aldrich); sn-G3P (Cayman Chemical); the GPD2 inhibitor iGP-1 - Calbiochem (Sigma-Aldrich); and antioxidants, including 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO; Sigma-Aldrich), mitochondria-targeted TEMPO (MitoTEMPO; Sigma-Aldrich), MitoQ (Cayman Chemical), and MitoQH2 (Cayman Chemical).
Wu S., Mao C., Kondiparthi L., Poyurovsky M.V., Olszewski K, & Gan B. (2022). A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria. Proceedings of the National Academy of Sciences of the United States of America, 119(26), e2121987119.
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