For BLI and quantification of lung metastasis burden, an IVIS Spectrum system (CaliperLS; Perkin-Elmer, Hopkinton, MA, USA) was used with software provided by the manufacturer (Living Image version 4.4.17504). D-luciferin (in PBS, 126 mg/kg) was injected intraperitoneally, acquisition of consecutive frames was started immediately thereafter until maximum signal intensity was reached, measured as photon flux per second through a region of interest (2.9 cm × 1.8 cm) covering the lungs. Image acquisition numbers and times varied between 10 and 15 frames of 30–60 s each, depending on optimal acquisition settings as a function of signal intensity intrinsic to lung metastasis grade.
Ivis spectrum system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Spectrum system is a preclinical imaging platform designed for in vivo bioluminescence and fluorescence imaging. It enables non-invasive, real-time visualization and quantification of biological processes in small animal models.
Automatically generated - may contain errors
Lab products found in correlation
Living image software, by PerkinElmer (20 mentions)
Living image 4, by PerkinElmer (11 mentions)
D luciferin, by PerkinElmer (5 mentions)
D luciferin, by Promega (4 mentions)
Mw 3000, by Thermo Fisher Scientific (3 mentions)
D luciferin, by Merck Group (3 mentions)
Nsg mice, by Charles River Laboratories (2 mentions)
D luciferin, by GoldBio (2 mentions)
Xenolight d luciferin k salt bioluminescent substrate, by PerkinElmer (2 mentions)
Nunc microwell, by Thermo Fisher Scientific (2 mentions)
Living image 3, by PerkinElmer (2 mentions)
Ivis spectrum, by PerkinElmer (2 mentions)
Osteosense 680ex, by PerkinElmer (2 mentions)
Iso urane, by Baxter (2 mentions)
Lsm 880, by Zeiss (2 mentions)
6460 triple quadruple mass spectrometer, by Agilent Technologies (1 mentions)
Cy7 nhs ester, by Lumiprobe (1 mentions)
Angiosense 750 ex, by PerkinElmer (1 mentions)
Luciferin, by GoldBio (1 mentions)
Living image software version 4, by PerkinElmer (1 mentions)
145 protocols using ivis spectrum system
1
Quantitative Lung Metastasis Imaging
2
In Vivo Fluorescence Imaging of Cas9
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Alexa Fluor 647–labeled Cas9 was used for in vivo fluorescence imaging. Free L76-Cas9 or L76-Cas9–loaded MSCM-NF were injected into the tibia of BALB/c mice and were imaged with an IVIS Spectrum system (PerkinElmer) with excitation at 640 nm and emission at 650 to 670 nm.
3
Preparation and Application of NIRF Probe Targeting CD206
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The NIRF probe targeting surface CD206 was prepared by conjugating
Cy7 fluorescent dye to 2-amino-2-deoxymannose.55 (link) Briefly, 1 μmol of Cy7 NHS ester (Lumiprobe) was
conjugated with 10 μmol of mannosamine hydrochloride in 0.05
M sodium borate buffer (pH 8.5). After incubation at 4 °C for
2 h in the dark, conjugated Man-Cy7 was purified by using a preparative
C18 HPLC column (Phenomenex) on a 321 preparative-LC system (Gilson).
The purified Man-Cy7 NIRF probe was confirmed using 1260 HPLC with
a 6460 triple-quadruple mass spectrometer (Agilent). For NIRF imaging,
mice were anesthetized with 2% isoflurane, followed by the intraperitoneal
injection of Man-Cy7 (5 nmol in 200 μL). After 4 h, in vivo imaging was performed using an IVIS spectrum system
(PerkinElmer), in which Cy7 fluorescence was measured at excitation
and emission wavelengths of 710 and 800 nm, respectively. In vivo image analysis was performed using Living Image
4.5 software (PerkinElmer).
Cy7 fluorescent dye to 2-amino-2-deoxymannose.55 (link) Briefly, 1 μmol of Cy7 NHS ester (Lumiprobe) was
conjugated with 10 μmol of mannosamine hydrochloride in 0.05
M sodium borate buffer (pH 8.5). After incubation at 4 °C for
2 h in the dark, conjugated Man-Cy7 was purified by using a preparative
C18 HPLC column (Phenomenex) on a 321 preparative-LC system (Gilson).
The purified Man-Cy7 NIRF probe was confirmed using 1260 HPLC with
a 6460 triple-quadruple mass spectrometer (Agilent). For NIRF imaging,
mice were anesthetized with 2% isoflurane, followed by the intraperitoneal
injection of Man-Cy7 (5 nmol in 200 μL). After 4 h, in vivo imaging was performed using an IVIS spectrum system
(PerkinElmer), in which Cy7 fluorescence was measured at excitation
and emission wavelengths of 710 and 800 nm, respectively. In vivo image analysis was performed using Living Image
4.5 software (PerkinElmer).
4
Bioluminescent Imaging of Transplanted Mice
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At different time points after transplantation, the mice were injected with luciferin (150 mg/kg body weight; PerkinElmer, 122799) and imaged with the IVIS Spectrum System (PerkinElmer) at the Biotechnology Resource Center at Cornell.
5
Combination Therapy for Recurrent Tumors
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First, 5 × 105 4T1-Luc cells were subcutaneously injected into the right flank of each BALB/c mouse. On day 10, the primary tumors were resected, retaining about 1% tumors to imitate the residual microtumors in the surgical bed. The mice were then randomly divided into six groups and received intravenous injections of PBS, FPNVs, OXA, O-FPNVs, TPNVs, or O-TPNVs (25 mg of FPNVs or TPNVs and 5 mg of OXA per kilogram body weight) every 3 days for four cycles. Recurrent tumor development was observed and analyzed within 60 days. The in vivo bioluminescence images of recurrent tumors were obtained using an IVIS spectrum system (PerkinElmer). Recurrent tumor sizes were measured using a digital caliper. When mice exhibited signs of impaired health or the tumor size exceeded 1500 mm3, individuals were euthanized. In addition, recurrent tumors were collected and further examined by flow cytometry and histological analyses.
6
Angiogenic Imaging in Injury Models
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Mice were anesthetized via inhaled isoflurane and depilated with Nair. Specimens were imaged before injection for baseline fluorescence levels at 770-nm wavelength using an IVIS Spectrum system (PerkinElmer 124262). Per manufacturer’s instructions, 100 μL of 20 μmol·L−1 AngioSense 750EX (PerkinElmer NEV10011EX) solution was injected into the tail vein followed by re-imaging 24 h post injection at excitation/emission of 750/800 nm. AngioSense protocol was performed 20 h post burn/tenotomy (n = 3/group).
7
Quantifying ADN Fluorescence in Mice
8
In vivo Bioluminescence Imaging of Pb-luc Infection
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In vivo imaging studies of bioluminescence activity from Pb-luc infected mice were performed using an IVIS Spectrum System (Perkin Elmer, Hanover, MD, USA), as described previously (38 (link)). LPB was detected at 24 and 48 h post challenge. Mice received 150 mg/kg of luciferin (Gold Biotechnology, St. Louis, MO, USA) i.p. in 200 µl of PBS. Three minutes post luciferin administration, mice were anesthetized by isoflurane inhalation. Mice were then positioned ventral side up in the IVIS chamber on a 37°C heating platform and continued to receive isoflurane through nose cones. Exposure time was set to 5 min or until complete saturation with f-stop = 1 and large binning setting. Bioluminescence emitted from the region of intensity (ROI) was measured as luminescence signal intensity in photons per second using the ROI settings of the Living Image® 3.0 software.
9
Quantifying Bioluminescence Signals in Mice
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After isoflurane-supported anesthesia, mice were intraperitoneally injected with 25 mg/kg L-012 solution. Bioluminescence images of each mouse were obtained under isoflurane anesthesia using an IVIS Spectrum system (PerkinElmer). For quantitative analyses, the included Living Image software was utilized to calculate the intensity of bioluminescent signals at standardized regions of interest (ROIs) for each mouse.
10
Tumor Targeting and Biodistribution Analysis
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Mice carrying a desired tumor size were included for analysis of biodistribution and tumor targeting of trimeric constructs and peptides. All trimeric constructs and peptides were injected intravenously via retrobulbar venous plexus in a final volume of 100 µL PBS buffer (3.34 nmol, 10 nmol, or 34.7 nmol/mice). Mice (n = 3 for each construct) were imaged in an IVIS Spectrum System (Perkin Elmer) using excitation range of 615–665 nm and monitoring emission signals at 695–770 nm. Imaging process was performed 1, 2, 3, or 6 h post-injection and after euthanization the tumor and specific organs were excised, imaged, weighed, and cryo-conservated for further analysis. Fluorescence intensity of regions of interest was quantified using Living Image® software (Perkin Elmer).
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