Caspase-1 activity (Biovision, Mountain View, CA, USA) was measured by a commercially available colorimetric assay. IL-1β production (R&D Systems, Minneapolis, MN, USA) and vascular endothelial growth factor (VEGF; Bender Medsystems, San Diego, CA, USA) were quantified through an ELISA using cellular supernatant. All these assays were performed according to manufacturer’s instructions.
Caspase 1 activity
Manufactured by Abcam
Sourced in United States
Caspase-1 activity assay measures the activity of caspase-1, a protease enzyme involved in the inflammatory response and cell death processes. The assay provides a quantitative assessment of caspase-1 activity in cell or tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β production, by R&D Systems (3 mentions)
Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (3 mentions)
Mouse il 1β elisa kit, by Thermo Fisher Scientific (1 mentions)
Urinary albumin, by Fortis Life Sciences (1 mentions)
Elisas, by R&D Systems (1 mentions)
Transam kit, by Active Motif (1 mentions)
Mpo activity, by Abcam (1 mentions)
Ldh assay kit, by Abcam (1 mentions)
6 protocols using caspase 1 activity
1
Quantification of Inflammatory Markers
2
Quantification of Inflammatory Markers
3
Nicotine Modulates Inflammatory Responses
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After nicotine treatment, cells were harvested and homogenized to extract proteins for caspase-1 activity assay by using a commercially available kit (Biovision). These data were expressed as the fold change compared with control cells. In addition, the cell supernatant was also collected to measure the IL-1β production by a mouse IL-1β ELISA kit (Bender Medsystems, Burlingame, CA) according to the protocol described by the manufacturer [21 (link), 50 (link)].
4
Quantifying Inflammatory Biomarkers
5
Plasma ALT and Cytokines Quantification
6
Lung Inflammation Biomarker Assays
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LDH activity in the lungs and BALFs was determined using an LDH assay kit (Abcam, UK) [36 (link)]. Nuclear proteins were extracted, and NF-κB activity was determined using the TransAM kit according to the manufacturer's instructions (Active Motif, USA). MPO activity (Abcam, UK) in fresh lung homogenates was detected by measuring absorbance at 412 nm; this was used as a biomarker for neutrophil infiltration. Caspase-1 activity (Abcam, UK) in the lungs or macrophages was detected using the fluorescent substrate YVAD-AFC and quantified by a multidetection reader. ASK1 activity was measured using an immune complex kinase assay with a His-MKK6 substrate as previously described [37 (link)].
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