Folic acid in brains was measured using a competitive receptor binding immunoassay (Chemiluminescent Immunoenzyme Assay Access Immunoassay System II; Beckman Coulter, Krefeld, Germany). The intra‐assay CV for folate was 3.8–6.5%.
Chemiluminescent immunoenzyme assay access immunoassay system 2
Manufactured by Beckman Coulter
Sourced in Germany
The Chemiluminescent Immunoenzyme Assay Access Immunoassay System II is a laboratory equipment designed for conducting automated immunoassay tests. It utilizes chemiluminescent detection technology to measure analyte levels in biological samples.
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3 protocols using chemiluminescent immunoenzyme assay access immunoassay system 2
1
Folic Acid in Brain Measurement
2
Folate Levels in Maternal-Fetal Tissues
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Folate concentrations in maternal serum were determined using a high-performance liquid chromatography tandem mass spectrometry system (HPLC-MS/MS). Folate contents of mouse maternal peripheral blood, mouse placentas and brains from human fetuses were measured using a competitive receptor-binding immunoassay (Chemiluminescent Immunoenzyme Assay Access Immunoassay system II, Beckman Coulter, Krefeld, Germany) according to the manufacturer’s instructions.
3
Folate Quantification in Embryonic Brain
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The level of folic acid was determined using a competitive receptor binding immunoassay (Chemiluminescent Immunoenzyme Assay Access Immuno-assay system II: Beckman Coulter, Krefeld, Germany) according to the standard protocol. Briefly, 15 mg of embryonic brain tissue samples was homogenized with 1 ml of extraction buffer (TRIS-buffered saline, A16792, BECKMAN, Germany). The homogenized tissue was ultra-sonicated for 3 min with 10-s ultra-sonication and 10-s interval (Bioruptor pico, Diagenode, Belgium). The samples were then centrifuged at 4°C, 12 000 rpm for 3 min, then 200 μl of supernatant was added to the sample cups for folate detection.
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