The largest database of trusted experimental protocols

Ezviable calcein am fluorometric cell viability assay kit

Manufactured by Abcam
Sourced in United States

The EZViable Calcein AM Fluorometric Cell Viability Assay Kit is a fluorometric-based assay used to determine cell viability. It utilizes the cell-permeant dye Calcein AM to measure the enzymatic activity of live cells.

Automatically generated - may contain errors

2 protocols using ezviable calcein am fluorometric cell viability assay kit

1

Assessing Cell Viability with Calcein AM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell viability assays were performed using our previously described method24 (link). Briefly, an EZViable Calcein AM Fluorometric Cell Viability Assay Kit (BioVision, Milpitas, CA, USA) was used to quantify the number of viable cells. MRMECs were cultured on sterile black 96 well plates under growth conditions. At 75% confluence, MRMECs were treated with 0–0.5 µM AS-Eng shRNA–lipid in complete medium, 0 to 0.5 µM NS-shRNA–lipid in complete medium or 70% ethanol as a positive control for 8 h. After treatment, the cells were washed with cold PBS (Life Technologies Corporations). MRMECs were then exposed to a buffered (1:500) calcein AM solution and incubated at 37 °C for 30 min. Fluorometric readings were performed using a microplate reader (Biotek; Winooski, VT). Fluorescence intensity was plotted on the Y-axis and represented as % live cells. Experiments were performed at least three times with n = 3 for each experimental group.
+ Open protocol
+ Expand
2

Calcein AM Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
An EZViable Calcein AM Fluorometric Cell Viability Assay Kit (BioVision; Milpitas, CA) was used to quantify the number of viable cells. MRMECs were cultured on sterile black 96-well plates under growth conditions. At 75% confluence, MRMECs were treated with 0 to 5 nM hAuNP in complete medium, citrate capped GNP (5 nM) in complete medium, or 70% ethanol as a positive control for 8 hrs. After treatment, the cells were washed with cold PBS (Life Technologies). MRMECs were then exposed to a buffered (1:500) calcein AM solution and incubated at 37°C for 30 minutes. Fluorometric readings were performed using a microplate reader (Biotek). Fluorescence intensity was plotted on the Y-axis and represented as % live cells. Experiments were performed at least three times with three replicates for each experimental group.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!