Naltrexone drug, undecylenic acid (10-undecenoic acid) and mPEG 2000 were purchased from Fluka (Darmstand, Germany). ε-CL (98%), 2-mercaptoethanol (99%), 2,2-dimethoxy-2-phenylacetophenone (DMPA) (99%), tin(II) 2-ethylhexanoate (Sn(Oct)2) (95%), PEG 1500, Poly(vinyl alcohol) (PVA), sodium chloride (NaCl), and disodium hydrogen phosphate (Na2HPO4) were supplied from Sigma-Aldrich Chemical Co. (Steinheim, Germany). Potassium dihydrogen phosphate (KH2PO4) and dichloromethane (DCM, CH2Cl2 ≥ 99.8% pure) was obtained from Chem-Lab and Merck Analytics (Kenilworth, NJ, USA), respectively. The stabilizer Tween 20 (Polysorbate 20) sorbitan monolaurate, were kindly supplied by Pharmathen S.A. (Athens, Greece). All the other reagents were of analytical grade and were used as received without further purification.
Disodium hydrogen phosphate na2hpo4
Manufactured by Merck Group
Sourced in Germany, United States, Ireland, Hungary
Disodium hydrogen phosphate (Na2HPO4) is a chemical compound that is commonly used as a buffer in various laboratory applications. It is a white, crystalline powder that is soluble in water and has a neutral pH. Disodium hydrogen phosphate is a versatile compound that can be used to maintain a specific pH in aqueous solutions, which is important for various experimental and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Sodium dihydrogen phosphate nah2po4, by Merck Group (8 mentions)
Sodium hydroxide (naoh), by Merck Group (7 mentions)
Sodium chloride (nacl), by Merck Group (5 mentions)
Citric acid, by Merck Group (4 mentions)
Acetonitrile, by Merck Group (4 mentions)
Methanol, by Merck Group (4 mentions)
Potassium dihydrogen phosphate kh2po4, by Merck Group (4 mentions)
Water purification system, by Merck Group (3 mentions)
Acetic acid, by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Merck Group (3 mentions)
Elix reference water purification system, by Merck Group (2 mentions)
N 2 hydroxyethylpiperazine n 2 ethanesulphonic acid hepes buffer, by Hampton Research (2 mentions)
Human serum albumin (hsa), by Merck Group (2 mentions)
Isopropanol, by Merck Group (2 mentions)
Direct q3 uv apparatus, by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Oxalic acid, by Merck Group (2 mentions)
Trypsin, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
35 protocols using disodium hydrogen phosphate na2hpo4
1
Synthesis of Naltrexone-Loaded Polymeric Nanoparticles
2
Electrochemical Glucose and Lactate Biosensing
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Selectophore grade sodium ionophore X, bis(2-ethylehexyl) sebacate (DOS), sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (Na-TFPB), high–molecular weight polyvinyl chloride (PVC), tetrahydrofuran, valinomycin (potassium ionophore), sodium tetraphenylborate (NaTPB), cyclohexanone, polyvinyl butyral resin BUTVAR B-98 (PVB), 3,4-ethoxylenedioxythiophene (EDOT), NaPSS, glucose oxidase (from Aspergillus niger), l -lactate oxidase (>80 activity units/mg), AA, UA, lactate, and glucose were purchased from J&K Scientific. Chitosan, acetic acid, disodium hydrogen phosphate (Na2HPO4), potassium dihydrogen phosphate (KH2PO4), sodium chloride (NaCl), potassium chloride (KCl), potassium ferricyanide (III), and potassium ferricyanide (II) were obtained from Sigma-Aldrich. All other chemicals (analytical reagent grade) were commercially available and used without further purification. All solutions were prepared using deionized water (18.3 megohm·cm) produced from a Millipore water purification system.
3
Synthesis of Gold Nanoparticles
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Hydrogen tetrachloroaurate(III) trihydrate (chloroauric acid, HAuCl4·3H2O) was purchased from Fisher Chemical, Ireland. N-2-hydroxyethylpiperazine-N-2-ethanesulphonic acid (HEPES) buffer was purchased from Hampton Research, US. Sodium phosphate monobasic (NaH2PO4) and disodium hydrogen phosphate (Na2HPO4) were purchased from Sigma Aldrich, Ireland. Deionized water, deionized using Elix® Reference Water Purification System from Millipore, Ireland, was used for all experiments and solution preparations.
4
Formulation Development of Meloxicam-HSA Complexes
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Meloxicam (MEL, 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-benzothiazine-3-carboxamide-1,1-dioxide) was donated by EGIS Pharmaceuticals Plc. (Budapest, Hungary) for research work. Human serum albumin (HSA, lyophilized powder, purity > 97%), fluorescein isothiocyanate-labelled HSA (FITC-HSA), Tween 80 (Tween), P407, disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), polar brain lipid extract, cholesterol, mucin from porcine stomach (Type III), and all reagents for cell line studies were purchased from Sigma Aldrich Co. Ltd. (Budapest, Hungary) if not indicated otherwise. Analytical grade solvents such as methanol, dimethyl sulfoxide (DMSO) and dodecane were purchased from Molar Chemicals (Budapest, Hungary). Sodium hyaluronate (NaHA, Mw = 1400 kDa) was obtained from Gedeon Richter Plc. (Budapest, Hungary). In all experiments, water was purified by the Millipore Milli-Q® 140 Gradient Water Purification System.
5
SARS-CoV-2 Spike Glycoprotein Aptamer Synthesis
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The SARS-CoV-2 spike glycoprotein–specific aptamers were custom-synthesized by Bioneer (South Korea). Besides, the sequences are given as follows [58 , 59 (link)]: 5′-NH2-TCGCTCTTTCCGCTTCTTCGCGGTCATTGTGCATCCTGACTGACCCTAAGGTGCGAACATCGCCCGCGTAAGTCCGTGTGTGCGAA-3′.
Analytical grade of disodium hydrogen phosphate (Na2HPO4), sodium hydroxide (NaOH), sodium chloride (NaCl), 1,3,5-benzene tricarboxylic acid (C9H6O6, H3BTC), potassium nitrate (KNO3), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), monosodium dihydrogen phosphate (NaH2PO4), ethanol (C2H6O), and all other reagents were bought from Sigma-Aldrich or Merck and they were used sans additional purification. A redox probe was used which was prepared as a 0.01 M phosphate-buffered solutions (PBS) with pH = 7.4 containing K3Fe(CN)6/K4Fe(CN)6 (5 mM) in a 1 to 1 ratio and the all experiments were performed at room temperature.
Analytical grade of disodium hydrogen phosphate (Na2HPO4), sodium hydroxide (NaOH), sodium chloride (NaCl), 1,3,5-benzene tricarboxylic acid (C9H6O6, H3BTC), potassium nitrate (KNO3), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), monosodium dihydrogen phosphate (NaH2PO4), ethanol (C2H6O), and all other reagents were bought from Sigma-Aldrich or Merck and they were used sans additional purification. A redox probe was used which was prepared as a 0.01 M phosphate-buffered solutions (PBS) with pH = 7.4 containing K3Fe(CN)6/K4Fe(CN)6 (5 mM) in a 1 to 1 ratio and the all experiments were performed at room temperature.
6
Polymer-Based Membrane Fabrication
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Polyethersulfone (PES Ultrason E6020P with Mw = 75,000 g/mol) was purchased from Solvay Specialty Polymers, (Jurong Island, Singapore). Thiourea (CH4N2S) was purchased from HmbG (Kuala Lumpur, Malaysia). N-methyl-2-pyrolidone (NMP) and silver nitrate (AgNO3) were purchased from Merck, di-sodium hydrogen phosphate (Na2HPO4) was purchased from Sigma Aldrich (Petaling Jaya, Malaysia), and methyl orange (MO) was purchased from Bendosen Laboratory Chemicals (Kuala Lumpur, Malaysia). Distilled water was used throughout the study. All chemicals are of analytical grade and were used without any further purification.
7
HPLC Analysis of Medicinal Triterpenoids
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The pharmacopeial standards of arjunic acid, arjunolic acid, arjungenin, arjunglucoside I, sericic acid and arjunetin were purchased from Sigma Aldrich (St. Louis, MO, USA; p.a.). The organic modifiers for the micellar mobile phase, i.e., acetonitrile and isopropanol, as well as surfactants: polyoxyethylene (23) lauryl ether (Brij35) and dodecyl sodium sulfate (SDS), were purchased from Merck (Darmstadt, Germany; p.a.). The buffer components, i.e., citric acid and disodium hydrogen phosphate (Na2HPO4), were purchased from Sigma Aldrich (Sigma Aldrich, St. Louis, MO, USA; p.a.). Distilled water was obtained from the Direct-Q3 UV apparatus (Millipore, Warsaw, Poland).
8
Spectrophotometric Analysis of Oxidants
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CLQ and diammonium 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) were obtained from Aladdin Industrial Corporation (Shanghai, China) with purity higher than 98%. K2FeO4 of high purity (>92%) was synthesized by a wet oxidation method in the laboratory [36] (link). The Fe(VI) stock solutions were freshly prepared within 30 min prior to their use. Other reagents at least analytical purity including disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), sodium tetraborate decahydrate (Na2BO4), sodium chloride (NaCl), sodium nitrate (NaNO3), sodium bicarbonate (NaHCO3), sulphuric acid (H2SO4), glacial acetic acid (CH3COOH) and sodium thiosulfate (Na2S2O3) were purchased from Sigma-Aldrich (St. Louis, USA). Purified water was provided from JD Co. (Beijing, China). All the buffer solution was prepared using phosphate and borate to adjust the pH from 6.5 to 10.5.
9
Diabetes Induction Protocol Using STZ
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Bovine serum albumin (BSA), d -(+)-glucose, disodium hydrogen phosphate (Na2HPO4), ethanol, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), mannitol, methanol, pioglitazone hydrochloride (Pio), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), streptozotocin (STZ), sodium chloride (NaCl), sodium citrate dehydrate (C6H5O7Na3·2H2O), and sucrose, were purchased from Sigma (St Louis, MO, USA). 1,4-Dithiothreitol (DTT) was obtained from Merk KGaA (Darmstadt, Germany). The protease inhibitor cocktail tablet was purchased from Roche (Mannheim, Germany). The Bio-Rad protein assay dye reagent was obtained from Bio-Rad Laboratories (Richmond, VA, USA). All of the chemicals used in this study are of analytical grade.
10
Electrochemical Detection of 5-Fluorouracil
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All the chemicals and reagents [thiophene monomer (C4H4S) (≥99% SRL), ferric chloride (FeCl3) (≥98% AR), boron nitride bulk (h-BN) (≥99% SRL), sodium hydroxide (NaOH) (≥98% FISHER), acetonitrile, ethanol, methanol, 5-fluorouracil (C4H3FN2O2) (≥99% SRL), uric acid (C5H4N4O3) (≥99% SRL), lactose (C12H22O11) (≥98% SRL), oxalic acid (≥99.5% AR), ascorbic acid (C6H8O6) (≥99.7% SRL), dopamine (C8H11NO2) (≥99% SRL), l -cysteine (C3H7NO2S) (≥99% SRL), glucose (C6H12O6) (≥99% SRL), potassium ferricyanide (C6N6FeK3) (≥99% SRL), potassium ferrocyanide (C6FeK4N6) (≥99% SRL), potassium chloride (KCl) (99.8% Fisher), monosodium hydrogen phosphate (NaH2PO4) (≥99% SRL), and disodium hydrogen phosphate (Na2HPO4) (≥99% SRL)] are purchased in Sigma Aldrich and Alfa Aesar. To create a stock solution, ultrapure deionized water was used. NaH2PO4 and Na2HPO4 were combined to create 0.1 M phosphate buffer (PBS) (pH = 7.0) for the detection of 5-Fu. The human blood serum sample was collected from SRM Medical College Hospital and Research Center, Kattankulathur, Tamil Nadu, for real sample analysis. All the experiments were done by using Milli-Q-water (18.2 MΩ cm @ 25 ± 2 °C).
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