5973 ms system

Manufactured by Agilent Technologies

Sourced in Germany

The 5973 MS system is a quadrupole mass spectrometer designed for use in analytical chemistry applications. It is capable of performing electron ionization and chemical ionization mass spectrometry. The system is equipped with a high-performance vacuum system and an advanced detector system to provide sensitive and accurate mass analysis.

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Lab products found in correlation

Api4000 ms ms system, by Thermo Fisher Scientific (2 mentions) 1100 hplc system, by Thermo Fisher Scientific (2 mentions) 7693a automatic liquid sampler, by Agilent Technologies (1 mentions) 1100 hplc system, by Agilent Technologies (1 mentions) 6890 gas chromatograph, by Agilent Technologies (1 mentions)

Spelling variants of this product

MS 5973 (15 citations) Agilent 6890 GC-5973 MS system (4 citations) Agilent 5973 MS System (4 citations) 6390N/5973 GC/MS system (3 citations) 6890 GC/ 5973 MS single quadrupole system (2 citations) Agilent 5973 GC/MS system (2 citations) 5973 MS Detection and Chemstation Data System (2 citations)

8 protocols using 5973 ms system

GC-MS Analysis of 13C Metabolites

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¹³C metabolism labeling experiments have previously been documented [23 (link)]. Metabolite extracts were collected and 2ul of metabolite extract samples were injected for the GC-MS analysis using an Agilent 6980 GC coupled to an Agilent 5973 MS system. Relative metabolite abundances were determined by normalizing abundances of each metabolite to the internal standard and to cell number.

Zhang M., Pan Y., Tang D., Dorfman R.G., Xu L., Zhou Q., Zhou L., Wang Y., Li Y., Yin Y., Kong B., Friess H., Zhao S., Wu J.L., Wang L, & Zou X. (2019). Low levels of pyruvate induced by a positive feedback loop protects cholangiocarcinoma cells from apoptosis. Cell Communication and Signaling : CCS, 17, 23.

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Cholesterol Profiling of T Cells

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Splenic T cells from female Ldlr^−/− and T-Abc^dkoLdlr^−/− mice fed a chow diet for 28 weeks, and young (3 months) and aged (24 months) wild-type male and female mice were isolated as described above. Cholestanol-D5 was added to the T cells as internal standard and cholesterol was extracted using hexane. For T cells from Ldlr^−/− and T-Abc^dkoLdlr^−/− mice, samples were split for measurement of either total or free cholesterol content using Gas Chromatography—Mass Spectrometry (7890B GS system, 5973 MS system, and 7693 A automatic liquid sampler from Agilent; positive chemical ionization mode with 5% ammonia in methane as reaction gas). A polar DB-WAXetr (30 m × 0.25 mm × 0.25 µm) column was used. CE content was calculated by subtracting free cholesterol from total cholesterol. Cholesterol content was normalized to cellular protein content measured by the Lowry assay. For T cells of young and aged wild-type mice, total cholesterol content was assessed using the same procedure.

Bazioti V., La Rose A.M., Maassen S., Bianchi F., de Boer R., Halmos B., Dabral D., Guilbaud E., Flohr-Svendsen A., Groenen A.G., Marmolejo-Garza A., Koster M.H., Kloosterhuis N.J., Havinga R., Pranger A.T., Langelaar-Makkinje M., de Bruin A., van de Sluis B., Kohan A.B., Yvan-Charvet L., van den Bogaart G, & Westerterp M. (2022). T cell cholesterol efflux suppresses apoptosis and senescence and increases atherosclerosis in middle aged mice. Nature Communications, 13, 3799.

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GC-MS Analysis of Metabolites

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Metabolism experiments were carried out as described previously [22] (link). Metabolite extracts were collected. A total of 2 μl of metabolite extracts were injected for gas chromatography-mass spectrometer (GC-MS) analysis using an Agilent 6980 GC coupled to an Agilent 5973 MS system. Relative metabolite abundances were investigated by comparing the abundance of each metabolite with internal standards and cell protein standards.

Xu L., Wang L., Zhou L., Dorfman R.G., Pan Y., Tang D., Wang Y., Yin Y., Jiang C., Zou X., Wu J, & Zhang M. (2019). The SIRT2/cMYC Pathway Inhibits Peroxidation-Related Apoptosis In Cholangiocarcinoma Through Metabolic Reprogramming. Neoplasia (New York, N.Y.), 21(5), 429-441.

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Lipid Profiling in Pancreatic Tissues

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Gas chromatography-mass spectrometry (GC–MS; Agilent 6890 GC coupled to an Agilent 5973 MS System, Waldbronn, Germany) and liquid chromatography-MS/MS (LC–MS/MS; Agilent 1100 HPLC-System, Darmstadt, Germany) were applied for the determination of fatty acids, triglycerides, cholesterol, phospholipids and eicosanoid levels.
Fatty acids, triglycerides, cholesterol, phospholipids and eicosanoid were extracted from pancreatic tissues respectively by liquid/liquid extraction and solid/liquid extraction. For further details, please see the Supplementary materials and methods.

Cao R.C., Yang W.J., Xiao W., Zhou L., Tan J.H., Wang M., Zhou Z.T., Chen H.J., Xu J., Chen X.M., Jin Y.C., Lin J.Y., Zeng J.L., Li S.J., Luo M., Hu G.D., Jin J., Yang X.B., Huo D., Zhou J, & Zhang G.W. (2022). St13 protects against disordered acinar cell arachidonic acid pathway in chronic pancreatitis. Journal of Translational Medicine, 20, 218.

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GC-MS Analysis of Cyanobacteria and Plants

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Cyanobacteria cells were harvested by centrifugation at 5000× g for 10 min at 4 °C and washed three times with distilled water. The pellets were then dried. The plant samples (0.3 g) were weighed and used for the subsequent analysis. The cyanobacteria cells and plant samples were transmethylated, extracted and analyzed by a gas chromatography/mass spectrometry (GC/MS) system using an Agilent 5973 MS system coupled with an Agilent 6890 gas chromatograph fitted with an HP-SMS capillary column (30 × 0.25 mm, film thickness 0.25 µm) [31 (link),32 (link)]. Helium was used as the carrier gas at a flow rate of 1 mL min⁻¹ with an injector temperature of 250 °C, a split ratio of 50:1 and a temperature program of 120 °C to 220 °C at a rate of 4 °C min⁻¹.

Li D., Li K., Zhou G, & He S. (2023). Effects of Temperature and Salt Stress on the Expression of delta-12 Fatty Acid Desaturase Genes and Fatty Acid Compositions in Safflower. International Journal of Molecular Sciences, 24(3), 2765.

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Mass Spectrometry Analysis of Plasma Lipids

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Three types of mass spectrometry analyses were applied. Gas chromatography–mass spectrometry (GC-MS; Agilent 6890 GC coupled to an Agilent 5973 MS System, Waldbronn, Germany) and liquid chromatography–MS/MS (LC–MS/MS; Agilent 1100 HPLC-System coupled to an Applied Biosystems API4000 MS/MS-System, Darmstadt, Germany).25 (link) Solid-phase extraction-LC–MS/MS (SPE-LC–MS/MS; Symbiosis Pharma, Spark, Emmen, The Netherlands) coupled to an Applied Biosystems API4000 MS/MS-System was used for the determination of steroid levels.26–28 (link)
Total lipids were extracted from plasma by liquid/liquid extraction using chloroform/methanol. The lipid extracts were subsequently fractionated by normal phase liquid chromatography into 11 different lipid groups.27 (link) For further details, see online supplementary material methods.

Mayerle J., Kalthoff H., Reszka R., Kamlage B., Peter E., Schniewind B., González Maldonado S., Pilarsky C., Heidecke C.D., Schatz P., Distler M., Scheiber J.A., Mahajan U.M., Weiss F.U., Grützmann R, & Lerch M.M. (2017). Metabolic biomarker signature to differentiate pancreatic ductal adenocarcinoma from chronic pancreatitis. Gut, 67(1), 128-137.

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Quantification of Metabolite Extracts

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The metabolite extracts were collected. A total of 2 μL metabolite extracts were injected for gas chromatography‐mass spectrometer (GC‐MS) analysis by an Agilent 6980 GC coupled to an Agilent 5973 MS system. The relative abundances of the metabolites were determined by comparing the abundance of each metabolite with internal standards and cell protein standards.

Xu L., Li Y., Zhou L., Dorfman R.G., Liu L., Cai R., Jiang C., Tang D., Wang Y., Zou X., Wang L, & Zhang M. (2019). SIRT3 elicited an anti‐Warburg effect through HIF1α/PDK1/PDHA1 to inhibit cholangiocarcinoma tumorigenesis. Cancer Medicine, 8(5), 2380-2391.

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Targeted Serum Metabolite Profiling

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Targeted metabolite pro ling of the serum samples which passed quality control was performed at a specialized metabolomics lab using a commercially available kit. The kit uses a protocol based on a 1phase extraction of the blood samples followed by gas chromatography mass spectrometry (GC-MS) (Agilent 6890 GC coupled to an Agilent 5973 MS-System) and liquid chromatography tandem-mass spectrometry (LC-MS/MS) (Agilent 1100 HPLC-System coupled to an Applied Biosystems API4000 MS/MS-System) analysis as previously described [28] . The analytical protocol was designed for routine measurement in the clinical practice setting; however, it is currently only available in specialized labs equipped with MS technology. The samples were stored at - 80 °C and transferred on dry ice prior to analysis. The three CLP metabolomic features and NT-proBNP measurements, were generated at baseline, only for the previously mentioned samples (n = 280). NT-proBNP was a measured using commercially available assays (Elecsys, Roche Diagnostics).

McGranaghan P., Saxena A., Westermann D., Rubens M., Appunni S., Salami J., Veledar E., Lacour P., Blaschke F., Obradović D., Loncar G., Tahirović E., Edelmann F., Pieske B., & Heidecke H. (2021). Performance of A Metabolomic Biomarker Score Compared to Three Prognostic Scores in Chronic Heart Failure.

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