The lyophilized extract was dissolved in water in a concentration of 100 mg/mL and the analysis was performed by ultra-fast liquid chromatography (UFLC) coupled to a photodiode array detector (PDA), through a methodology described by Pereira et al. [31 (link)], using a Shimadzu 20A series UFLC (Shimadzu Corporation, Kyoto, Japan). The compounds were separated using a SphereClone reverse phase C18 column (250 mm × 4.6 mm, 5 µm, Phenomenex, Torrance, CA, USA), at 35 °C and a flow rate of 0.8 mL/min of sulphuric acid (3.6 mM), through isocratic elution. The results were expressed in mg per g of extract.
Sphereclone reverse phase c18 column
Manufactured by Phenomenex
Sourced in United States
The SphereClone reverse phase C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The column features spherical silica particles with a C18 bonded phase, providing good chromatographic efficiency and resolution. The column is suitable for a variety of applications in analytical chemistry, pharmaceuticals, and other industries that require reliable and reproducible HPLC separations.
Automatically generated - may contain errors
Lab products found in correlation
20a series uflc, by Shimadzu (2 mentions)
Quinic acid, by Merck Group (1 mentions)
Oxalic acid, by Merck Group (1 mentions)
Shikimic acid, by Merck Group (1 mentions)
Malic acid, by Merck Group (1 mentions)
Fumaric acid, by Merck Group (1 mentions)
Succinic acid, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
20a series, by Shimadzu (1 mentions)
4 protocols using sphereclone reverse phase c18 column
1
Quantitative UFLC-PDA Analysis of Extracts
2
Quantifying Organic Acids in Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organic acids were extracted from the lyophilized fruit and analyzed via ultra-fast liquid chromatography (UFLC) coupled to photodiode array detector (PDA), employing a Shimadzu 20A series UFLC (Shimadzu Cooperation), according to Barros et al. [8 (link)]. Separation was achieved using a SphereClone reverse phase C18 column (250 mm × 4.6 mm, 5 µm, Phenomenex, Torrance, CA, USA), with an isocratic elution using sulphuric acid (3.6 mM) at a flow rate of 0.8 mL/min, operating at 35 °C. The results were expressed in g per 100 g of fresh fruit.
3
Macroalgae Nutritional Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to the AOAC methods [38] , protein, fat, carbohydrates and ash contents were determined on the three selected macroalgae species. Total carbohydrates were calculated by difference and energetic value was calculated using Equation (1):
The organic acids content of the three macroalgae samples was determined using an Ultra-Fast Liquid Chromatography (UFLC, Shimadzu 20A series, Kyoto, Japan) coupled to a photodiode array detector [39] . A SphereClone reverse phase C18 column (5 µm, 250 mm × 4.6 mm i.d., Phenomenex, Torrance, CA, USA) was used for separation at 35 • C. The mobile phase was sulfuric acid 3.6 mM at a flow rate of 0.8 mL/min. To quantify the compounds, calibration curves plotted using commercial standards (L-(+)-ascorbic acid, citric acid, malic acid, oxalic acid, shikimic acid, succinic acid, fumaric acid and quinic acid) procured from Sigma-Aldrich (St. Louis, MO, USA) were used. All tests were carried out in duplicate, expressed in terms of mean ± standard deviation (SD) and expressed in g per 100 g of dry weight (dw).
The organic acids content of the three macroalgae samples was determined using an Ultra-Fast Liquid Chromatography (UFLC, Shimadzu 20A series, Kyoto, Japan) coupled to a photodiode array detector [39] . A SphereClone reverse phase C18 column (5 µm, 250 mm × 4.6 mm i.d., Phenomenex, Torrance, CA, USA) was used for separation at 35 • C. The mobile phase was sulfuric acid 3.6 mM at a flow rate of 0.8 mL/min. To quantify the compounds, calibration curves plotted using commercial standards (L-(+)-ascorbic acid, citric acid, malic acid, oxalic acid, shikimic acid, succinic acid, fumaric acid and quinic acid) procured from Sigma-Aldrich (St. Louis, MO, USA) were used. All tests were carried out in duplicate, expressed in terms of mean ± standard deviation (SD) and expressed in g per 100 g of dry weight (dw).
4
Quantification of Organic Acids by UFLC-DAD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organic acids were determined by ultra-fast liquid chromatography coupled to a photo diode array detector (UFLC-DAD), following a previously described methodology (Heleno et al., 2015) . Separation was achieved on a SphereClone reverse phase C 18 column (4.6 × 250 mm, 5 μm, Phenomenex, Torrance, CA, USA) thermostatted at 35 °C. The elution was performed with sulphuric acid (3.6 mM) using a flow rate of 0.8 ml/min. The identification was performed by comparing relative retention times and UV spectra with commercial standards. Quantification was accessed by area comparison of the peaks recorded at 215 nm with calibration curves obtained from commercial standards and results were expressed in g per 100 g dw.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!