Oct compound

Manufactured by Wuhan Servicebio Technology

Sourced in China

The OCT compound is a type of lab equipment used for optical coherence tomography (OCT) analysis. OCT is a non-invasive imaging technique that uses light to capture cross-sectional images of biological tissues. The core function of the OCT compound is to acquire high-resolution, three-dimensional images of structures within the sample.

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Lab products found in correlation

Dm5500b, by Leica (1 mentions) H 7650, by Hitachi (1 mentions) Cryostar nx50, by Thermo Fisher Scientific (1 mentions) Lsm 800, by Zeiss (1 mentions) Tunel apoptosis assay kit, by Beyotime (1 mentions) Anti cd68, by Abcam (1 mentions) Dihydroethidium dhe, by Beyotime (1 mentions) Ly6g antibody, by Wuhan Servicebio Technology (1 mentions) F4 80 antibody, by Wuhan Servicebio Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Hoechst 33342, by Beyotime (1 mentions) Triton x 100, by Merck Group (1 mentions) Lsm 880, by Zeiss (1 mentions) In situ cell death detection kit, by Roche (1 mentions)

6 protocols using oct compound

Evaluating Hepatic Fat Deposition and Ultrastructure

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Oil red O staining was used to evaluate fat deposition in the liver. Briefly, liver samples were fixed in a 10% formaldehyde solution for 24 h, then dehydrated in a 15% and 30% sugar solution at 4 °C. The dehydrated samples were embedded into an optimal cutting temperature (OCT) compound (Servicebio, Wuhan, China), and cut into 8 μm-thick sections using a freezing microtome (CRYOSTAR NX50, Thermo Scientific, Waltham, MA, USA). After that, sections were stained using oil red O and hematoxylin, then, they were observed and photographed under a microscope (DM5500B, Leica, Germany).
Transmission electron microscopy (TEM) analysis was conducted to observe hepatocellular ultrastructure. Samples were fixed in 2.5% glutaraldehyde solution overnight and post-fixed in osmic acid for 2 h at 4 °C. Then, the samples were dehydrated in gradient acetone solutions and embedded in epoxy resin. Ultrathin slices with a 60-nm thickness were produced, stained with uranyl acetate and lead citrate solutions, and observed under a TEM (Hitachi H-7650, Tokyo, Japan).

Dong Y., Li L., Xia T., Wang L., Xiao L., Ding N., Wu Y, & Lu K. (2022). Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress. Antioxidants, 11(7), 1276.

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Comprehensive Histological Evaluation of Cardiac Tissues

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Hearts were collected and fixed in 4% paraformaldehyde overnight and processed for routine paraffin histology. The 5-μm sections were stained with hematoxylin-eosin and picrosirius red. Wheat germ agglutinin (WGA) staining was used to evaluate the cross-sectional area of cardiomyocytes. To visualize ROS production within the myocardium, fresh hearts were immediately embedded in O.C.T. compound (Servicebio, Wuhan) and cut into 5 μm slices. Slices were stained with 5 μM dihydroethidium (DHE, Beyotime, Nantong) for 1 hour in the dark at room temperature and then washed 3 times for 1 hour with Krebs-HEPES buffer. For apoptosis observation, heart slices were stained using a TUNEL apoptosis assay kit (Beyotime, Nantong). For immunofluorescence staining, heart slices were incubated overnight with a CD68 antibody (anti-CD68, Abcam, USA) and subsequently fluorescence-labeled with a secondary antibody. Images were viewed with confocal scanning microscopy (Zeiss, LSM 800), and the fluorescence intensity was estimated using ImageJ (NIH, USA).

Liao S., Tang Y., Yue X., Gao R., Yao W., Zhou Y, & Zhang H. (2021). β-Hydroxybutyrate Mitigated Heart Failure with Preserved Ejection Fraction by Increasing Treg Cells via Nox2/GSK-3β. Journal of Inflammation Research, 14, 4697-4706.

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Quantifying Apoptosis in Mid-Penile Tissue

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Mid‐penile tissue was fixed and embedded with optimal cutting temperature compound (OCT) compound (Servicebio, G6059), cut to 5 μm thickness. The tissue was then permeabilized with PBS containing 0.25% Triton TMX‐100 and stained with the in‐situ cell death assay kit (Servicebio, G1502‐50T) according to the manufacturer's instructions. The TUNEL+ cells/Hoechst 33342+ cells ratio was quantified using ImageJ software.

Yang W., Qiu C., Zhai J., Zhang W., Huang C., Shao J., Zhang J., Chen S., Miao X., Chen P., Wei B., Ren J, & Wei H. (2023). Ultrasound‐targeted microbubble destruction mediates PDE5i/NO integration for cavernosum remodeling and penile rehabilitation. Bioengineering & Translational Medicine, 8(5), e10568.

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Quantifying Hepatocyte Apoptosis and Immune Infiltration

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Mouse liver tissues were embedded in an optimal cutting temperature (OCT) compound (Servicebio) to make frozen sections. Serial sections of the tissues of 8 μm thickness were cut and used for the fluorescence observations. Double immunofluorescent staining was performed to evaluate immune cell infiltration with Ly6G antibody (Servicebio) and F4/80 antibody (Servicebio). The apoptosis of hepatocytes was detected using a terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay from a commercial kit (Yeasen), following the manufacturer’s instructions. The nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (Beyotime, Shanghai, China), and the positive cells were visualized using a fluorescence microscope.

Luan X., Chen P., Li Y., Yuan X., Miao L., Zhang P., Cao Q., Song X, & Di G. (2023). TNF-α/IL-1β-licensed hADSCs alleviate cholestatic liver injury and fibrosis in mice via COX-2/PGE2 pathway. Stem Cell Research & Therapy, 14, 100.

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Liver Tissue TUNEL Assay and Analysis

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Liver tissues were fixed, embedded with OCT compound (Servicebio, G6059), and sliced into 8 μm in thickness, then permeated with PBS containing 0.25% Triton^TM X-100 (Sigma-Aldrich, T8787) and stained with In Situ Cell Death Detection Kit (Roche, 11684795910) following the manufacturer’s instructions. Hoechst 33342 (Beyotime, C1022) was used to counterstain nuclei. Sections were visualized under the confocal microscope (ZEISS, LSM 880), and images were analyzed by ZEN 2012 software. Quantification for TUNEL⁺ cells/Hoechst 33342⁺ cells ratio and average nuclear diameter was conducted using ImageJ software (version 1.8.0).

Xu Y., Zhou Z., Kang X., Pan L., Liu C., Liang X., Chu J., Dong S., Li Y., Liu Q., Sun Y., Yu S, & Zhang Q. (2022). Mettl3-mediated mRNA m6A modification controls postnatal liver development by modulating the transcription factor Hnf4a. Nature Communications, 13, 4555.

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Posterior Eye Tissue Dissection

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The eyes were removed from the vials and washed in a series of graded sucrose solutions. The anterior tissues and the vitreous were removed by dissection, and the remaining eye cups were trimmed to gain access to the posterior tissues (Figure 1a). Two 5-mm-wide segments were cut out of the posterior tissues superior-inferiorly, with the first segment that was centered at the fovea and the second segment that was immediately nasal to the first segment containing the optic nerve head (Figure 1b). The segments were dried with filter paper, embedded in OCT compound (Servicebio, Wuhan, China), frozen, and sectioned every 10 μm nasal-temporally in a cryostat at -20 °C. Sections containing the nasal peripheral, nasal macular, fovea, and temporal macular tissues were particularly selected for further processing (Figure 1b). In addition, the blood vessel tissues were sectioned using the same procedure for further processing.Figure 1

Eye dissection. After removing the anterior tissues and the vitreous, the posterior landmarks became visible (a). Two tissue segments were dissected out based on the landmarks, and tissue sections were collected from four different areas (b).

Figure 1

Wang J., Zhang H., Ji J., Wang L., Lv W., He Y., Li X., Feng G, & Chen K. (2022). A histological study of atherosclerotic characteristics in age-related macular degeneration. Heliyon, 8(3), e08973.

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