Horseradish peroxidase hrp coupled secondary anti mouse and anti rabbit antibodies

Manufactured by Thermo Fisher Scientific

Horseradish peroxidase (HRP)-coupled secondary anti-mouse and anti-rabbit antibodies are laboratory reagents used in various immunoassay techniques. They are designed to bind to primary antibodies that have been raised against mouse or rabbit antigens, allowing for the detection and visualization of target proteins or molecules.

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Lab products found in correlation

Rabbit anti erk2, by Santa Cruz Biotechnology (2 mentions) Anti goat secondary antibody, by Santa Cruz Biotechnology (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Mouse anti flag m2 antibody, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Secondary anti rabbit, by Santa Cruz Biotechnology (1 mentions)

2 protocols using horseradish peroxidase hrp coupled secondary anti mouse and anti rabbit antibodies

Immunoblotting of Phosphorylated Proteins

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Lipofectamine 2000 and horseradish peroxidase (HRP)-coupled secondary anti-mouse and anti-rabbit antibodies were from ThermoFisher Scientific (Waltham, MA). Cycloheximide and mouse anti-FLAG (M2) antibody were from Sigma-Aldrich (St Louis, MI). FLT3 ligand (FL) was from ORF Genetics (Reykjavik, Iceland). Rabbit anti-phospho-AKT (pSer473), mouse anti-phosphotyrosine (4G10), mouse anti-mono-ubiquitin antibodies were from Abcam (Cambridge, UK), Merck Millipore (Billerica, MA) and Covance Research Products (Princeton, NJ), respectively. Rabbit anti-phospho-ERK1/2 (pThr202/pThr204), goat anti-AKT, rabbit anti-ERK2 and anti-goat secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). The phycoerythrin-labeled anti-FLT3, mouse anti-phospho-p38 and anti-p38 antibodies were from BD Transduction Laboratories (Franklin Lakes, NJ). Rabbit anti-FLT3 antibody was described previously [29 (link)].

Kazi J.U., Rupar K., Marhäll A., Moharram S.A., Khanum F., Shah K., Gazi M., Nagaraj S.R., Sun J., Chougule R.A, & Rönnstrand L. (2017). ABL2 suppresses FLT3-ITD-induced cell proliferation through negative regulation of AKT signaling. Oncotarget, 8(7), 12194-12202.

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Immunoblotting of Phospho-Signaling Proteins

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Reagents, antibodies and inhibitors: Horseradish peroxidase (HRP)-coupled secondary anti-mouse and anti-rabbit antibodies were from Thermo Fisher Scientific (Waltham, MA).
Rabbit anti-phospho-AKT (pSer473), rabbit anti-phospho-ERK1/2 (pThr202/pThr204), goat anti-AKT, rabbit anti-ERK2, anti-rabbit secondary and anti-goat secondary antibodies were from Santa Cruz Biotechnology (Dallas, TX). Mouse anti-phospho-p38 and anti-p38 antibodies were from BD Transduction Laboratories (Franklin Lakes, NJ). All inhibitors were from Selleck Chemicals (Houston, TX).

Gazi M., Moharram S.A., Marhäll A, & Kazi J.U. (2017). The dual specificity PI3K/mTOR inhibitor PKI-587 displays efficacy against T-cell acute lymphoblastic leukemia (T-ALL). Cancer letters, 392.

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