Mir 199b 5p

Manufactured by Thermo Fisher Scientific

Sourced in United States

MiR‐199b‐5p is a laboratory instrument used for the detection and quantification of the microRNA miR‐199b‐5p. It is designed to provide accurate and reliable measurement of this specific microRNA species within biological samples.

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Lab products found in correlation

Rnu48, by Thermo Fisher Scientific (2 mentions) Antibodies against gapdh, by Santa Cruz Biotechnology (1 mentions) Trametinib, by Selleck Chemicals (1 mentions) Taqman probes for gapdh, by Thermo Fisher Scientific (1 mentions) Mir 145 5p, by Thermo Fisher Scientific (1 mentions) Mir 204 5p, by Thermo Fisher Scientific (1 mentions) Vemurafenib, by Selleck Chemicals (1 mentions)

2 protocols using mir 199b 5p

Quantification of miRNA Expression Levels

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PCR quantification was carried out essentially as previously described.15, 16, 22 To quantify the expression level of miRNA, we utilized stem‐loop quantitative RT‐PCR (qRT‐PCR) for miR‐199a‐5p (assay ID: 000498; Applied Biosystems, Foster City, CA, USA), miR‐199b‐5p (assay ID: 000500, Applied Biosystems) and miR‐199‐3p (assay ID: 002304, Applied Biosystems) following the manufacturer's protocol. TaqMan probes and primers for Pri‐miR‐199a‐1 (Hs03302808_pri, Applied Biosystems), Pri‐miR‐199a‐2 (Hs03302922_pri, Applied Biosystems), Pri‐miR‐199b (Hs04227284_pri, Applied Biosystems) and ITGA3 (Hs01076873_m1, Applied Biosystems) were assay‐on‐demand gene expression products. mRNA and miRNA data were normalized to human GUSB (assay ID: Hs99999908_m1; Applied Biosystems) and RNU48 (assay ID: 001006; Applied Biosystems), respectively. The fold change was calculated using the delta–delta Ct method.

Koshizuka K., Hanazawa T., Kikkawa N., Arai T., Okato A., Kurozumi A., Kato M., Katada K., Okamoto Y, & Seki N. (2017). Regulation of ITGA3 by the anti‐tumor miR‐199 family inhibits cancer cell migration and invasion in head and neck cancer. Cancer Science, 108(8), 1681-1692.

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Molecular Targets in Cancer Biology

Cited 2 times

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Antibodies against GAPDH, Bcl-2 and VEGF were obtained from Santa Cruz Biotechnology. Vemurafenib and trametinib were obtained from Selleck Chemicals. TaqMan probes for GAPDH, VEGFA, TIMP2, PTPN14, HIF-1α, Bcl-2, CCL3, IL1b, CCL5, tgfB, CXCL8, CSF3, PDGFb, CCL20, miR-4443, miR-4488, miR-204-5p, miR-199b-5p, miR-630, miR-1234, ts-3676, miR-145-5p and RNU48 were purchased from Applied Biosystems. Primers relative to H3, RAD51, Notch1 and FOXM1 have been used in the work by Canu et al [18 (link)]. Western Blot analysis were performed as previously described [11 (link), 16 (link)].

Fattore L., Ruggiero C.F., Pisanu M.E., Liguoro D., Cerri A., Costantini S., Capone F., Acunzo M., Romano G., Nigita G., Mallardo D., Ragone C., Carriero M.V., Budillon A., Botti G., Ascierto P.A., Mancini R, & Ciliberto G. (2018). Reprogramming miRNAs global expression orchestrates development of drug resistance in BRAF mutated melanoma. Cell Death and Differentiation, 26(7), 1267-1282.

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