Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (St. Louis, MO). Rivastigmine L-tartrate was obtained from Tokyo Chemical Industry Co., LTD (TCI; Portland, OR). Complete mammalian protease inhibitor, donkey serum, Ficoll 400, and sodium dodecylphasphate (SDS) were obtained from (Sigma-Aldrich, MO). Reagents for total protein analysis with the bicinchoninic acid method were obtained from Pierce (Rockford, IL). For Western blot and immunohistochemistry, the mouse monoclonal antibody C-219 against P-gp was obtained from Covance Research Products (Dedham, MA); mouse monoclonal antibody against light chain LRP1 was obtained from Abcam (Cambridge, MA); goat polyclonal antibodies against actin (C-11), HRP-labeled secondary antibodies, rabbit polyclonal anti-GFAP antibody, rabbit polyclonal anti-EAAT (GLT-1) antibody, and CFL594-conjugated donkey anti-rabbit IgG were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Mouse monoclonal anti-PSD-95 antibody and rabbit polyclonal anti-SNAP-25 antibody were obtained from GeneTex, Inc (Irvine, CA). All other chemicals and reagents were of analytical grade and were readily available from commercial sources.
Rabbit polyclonal anti gfap antibody
Manufactured by Santa Cruz Biotechnology
The Rabbit polyclonal anti-GFAP antibody is a primary antibody that targets the Glial Fibrillary Acidic Protein (GFAP), a type III intermediate filament protein expressed in astrocytes and other glial cells in the central nervous system. This antibody can be used in various immunological techniques to detect and identify GFAP-expressing cells.
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2 protocols using rabbit polyclonal anti gfap antibody
1
Protein Expression Analysis in Cells
2
Immunohistochemical Analysis of Glioma Xenografts
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Immunohistochemical staining of paraffin sections was performed as described (Zhao et al., 2009 (link)). The human U-87 MG glioma cell xenograft tissues were cryosectioned at 8 μm thickness. Antigen retrieval was performed using 10 mM citrate buffer (pH 6.0), and endogenous peroxidase clearance was performed by incubation in 3% H2O2. Then, sections were blocked with 10% normal goat serum in PBS at room temperature for 30 min, and samples were subjected to incubation with the following primary antibodies: rat anti-human CHL1 antibody (1:100, cat. no. MAB2126, R&D Systems), rabbit polyclonal anti-PCNA antibody (1:200, cat. no. sc-7907, Santa Cruz), rabbit polyclonal anti-caspase-3 antibody (1:200, cat. no. sc-7148, Santa Cruz), rabbit polyclonal anti-GFAP antibody (1:500, cat. no. BA0056, Boster Biological Technology) at 4°C overnight. Bound antibody was visualized using the AEC method. Counterstaining was performed with Mayer’s hematoxylin. H&E (Zhongshan Goldbridge Biotechnology Co., LTD, Beijing, China) and immunohistochemical stainings were analyzed using a Jiangnan light microscope (DN-10B, Jiangnan, Nanjing, Jiangsu).
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