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Efluor450 anti mouse cd45 clone 30 f11

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EFluor450-anti-mouse CD45 (clone 30-F11) is a fluorescently-labeled antibody that binds to the mouse CD45 protein, which is expressed on the surface of mouse hematopoietic cells.

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Lab products found in correlation

Fitc anti mouse cd206 clone c068c2, by BioLegend (2 mentions) Anti cd16 cd32 abs clone 2.4g2, by Sungene Biotech (2 mentions) Percp cy5.5 anti mouse human cd11b clone m1 70, by BioLegend (2 mentions) Apc anti mouse cd86 clone gl 1, by BioLegend (2 mentions) Facsverse, by BD (2 mentions) Pe cy7 anti mouse f4 80 clone bm8, by BioLegend (2 mentions) Apc anti mouse cd11c clone hl3, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Fitc anti mouse cd86 clone gl1, by Cytek Biosciences (2 mentions) Pe anti mouse cd80 clone 16 10a1, by Cytek Biosciences (2 mentions) Fitc anti mouse cd4 clone gk1, by Cytek Biosciences (2 mentions) Percp cy5 anti mouse b220 clone ra3 6b2, by Cytek Biosciences (2 mentions) Apc cy7 anti mouse cd8 clone 53 6, by Cytek Biosciences (2 mentions) Fitc anti mouse cd8a clone 53 6, by Thermo Fisher Scientific (1 mentions) Moflo cell sorter, by Agilent Technologies (1 mentions) Liberase, by Roche (1 mentions)

Spelling variants of this product

CD45 (30-F11, (34 citations) CD45 (clone 30-F11, (31 citations) Anti-CD45 (clone 30-F11, (23 citations) Clone 30-F11 (22 citations) Anti-CD45 (30-F11, (15 citations) Anti-CD45-eFluor450 (8 citations) Anti-mouse CD45 eFluor450 (6 citations) CD45-eFluor450 (clone 30-F11, (4 citations) Anti-mouse CD45 (30-F11, (4 citations) Anti-mouse CD45 (clone 30-F11, (2 citations)

5 protocols using efluor450 anti mouse cd45 clone 30 f11

1

Macrophage Polarization Profiling in Muscle

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Transverse abdominal muscles were dissected, minced, and digested in 0.2% collagenase for 45 min. Single‐cell suspensions were incubated with purified anti‐CD16/CD32 Abs (clone 2.4G2, Sungene Biotech, Tianjin, China) for 15 min to block Fc receptors. After washing, cells were stained with eFluor 450 anti‐mouse CD45 (clone 30‐F11, Invitrogen, Carlsbad, CA), Percp‐Cy5.5‐anti‐mouse/human CD11b (clone M1/70, Biolegend, San Diego, CA), PE‐Cy7‐anti‐mouse F4/80 (clone BM8, Biolegend, San Diego, CA), APC‐anti‐mouse CD86 (clone GL‐1, Biolegend, San Diego, CA), FITC‐anti‐mouse CD206 (clone C068C2, Biolegend), or isotype controls at 4°C for 15 min and detected by flow cytometry (FACSVerse, BD). Data were analysed using FlowJo software (V10). Macrophages were identified as CD45+/CD11b+/F4/80+, and the percentage of pro‐inflammatory (CD86+) and anti‐inflammatory (CD206+) macrophages was analysed and shown.
Ding H., Chen S., Pan X., Dai X., Pan G., Li Z., Mai X., Tian Y., Zhang S., Liu B., Cao G., Yao Z., Yao X., Gao L., Yang L., Chen X., Sun J., Chen H., Han M., Yin Y., Xu G., Li H., Wu W., Chen Z., Lin J., Xiang L., Hu J., Lu Y., Zhu X, & Xie L. (2021). Transferrin receptor 1 ablation in satellite cells impedes skeletal muscle regeneration through activation of ferroptosis. Journal of Cachexia, Sarcopenia and Muscle, 12(3), 746-768.
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2

Identifying Pro- and Anti-inflammatory Macrophages

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Single-cell suspensions were incubated with purified anti-CD16/CD32 Abs (clone 2.4G2, Sungene Biotech, Tianjin, China) for 15 min to block Fc receptors. After wash, cells were stained with eFluor 450-anti-mouse CD45 (clone 30-F11, Invitrogen), Percp-Cy5.5-antimouse/human CD11b (clone M1/70, Biolegend), PE-Cy7-anti-mouse F4/80 (clone BM8, Biolegend), APC-anti-mouse CD86 (clone GL-1, Biolegend), FITC-anti-mouse CD206 (clone C068C2, Biolegend) or isotype controls at 4 for 15 min and detected by flow cytometry (FACSVerse, BD). Data were analyzed using FlowJo software (V10). Macrophages were identified as CD45 + /CD11b + /F4/80 + , and the percentage of pro-inflammatory (CD86 + ) and antiinflammatory (CD206 + ) macrophages were analyzed and shown.
Ding H., Chen S., Pan X., Dai X., Pan G., Li Z., Mai X., Tian Y., Zhang S., Liu B., Cao G., Yao Z., Yao X., Gao L., Yang L., Chen X., Sun J., Chen H., Han M., Yin Y., Xu G., Li H., Wu W., Chen Z., Lin J., Xiang L., Lu Y., Zhu X., & Xie L. (2020). Transferrin receptor (Tfr1) ablation in satellite cells impacts skeletal muscle regeneration through the activation of ferroptosis.
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3

Multicolor Flow Cytometry of BMMs and Tumor Samples

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Cultured BMMs were detached as described by Han et al. [14 (link)]. The BMMs were resuspended at 1.0 × 106 cells/1 ml in PBS and then incubated with FITC-anti-mouse CD86 (clone GL1, TONBO Biosciences, San Diego, CA), PE-anti-mouse CD80 (clone 16-10A1, TONBO), Alexa Fluor® 647-anti-mouse CD206 (clone MR5D3, BD Pharmingen). For immunoprofiling of tumor-bearing mice, single cell suspensions of Hepa1-6 tumors or the draining lymph nodes (dLN) at 1 × 106 cells/sample were immunostained with a combination of some of the following antibodies, such as FITC-anti-mouse CD4 (clone GK1.5, Tonbo), PE-anti-mouse CD11b (clone M1/70, BD), PerCP-Cy5-anti-mouse-B220 (clone RA3-6B2, Tonbo), APC-anti-mouse-CD11c (clone HL3, BD), eFluor450-anti-mouse CD45 (clone 30-F11, eBioscience), APC-Cy7-anti-mouse-CD8 (clone 53-6.7, Tonbo), eFluor450-anti-mouse CD44 (clone IM7, eBioscience), APC-anti-mouse CD62L (clone MEL-14, eBioscience), eFluor450-anti-mouse CD8 (clone 53-6.7, eBioscience), and eFluor660-anti-mouse CD107a (clone 1D4B, eBioscience). Data of the stained samples were acquired using an LSR II flow cytometer (BD) and analyzed using the software FlowJo (Tree Star Inc., Ashland, OR).
Han Z., Liu S., Lin H., Trivett A.L., Hannifin S., Yang D, & Oppenheim J.J. (2019). Inhibition of murine hepatoma tumor growth by cryptotanshinone involves TLR7-dependent activation of macrophages and induction of adaptive antitumor immune defenses. Cancer Immunology, Immunotherapy, 68(7), 1073-1085.
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4

Isolation and Sorting of Tumor-Infiltrating CD4+ and CD8+ T Cells

Cited 1 time
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Tumors were bisected and one half of each tumor was used for DNA extraction, whereas the other half from the 4 animals in each treatment group was pooled, minced into small pieces and digested with 0.2 mg/mL DNAse and 1.67 Wunsch U/mL Liberase (Roche) using established protocols (24 (link), 29 (link)). Cell suspensions were filtered through a 40 µM cell strainer, lysed for red blood cells and incubated with anti-mouse CD16/32 (Fc block) prior to staining. Cells were stained with eFluor450 anti-mouse CD45 Clone 30-F11, APC anti-mouse CD4 Clone RM4–5 and FITC anti-mouse CD8a Clone 53–6.7 (eBioscience). CD4+ and CD8+ T cells were sorted using MoFlo cell sorter (DakoCytomation, Carpinteria, CA).
Rudqvist N.P., Pilones K.A., Lhuillier C., Wennerberg E., Sidhom J.W., Emerson R.O., Robins H.S., Schneck J., Formenti S.C, & Demaria S. (2017). Radiotherapy and CTLA-4 blockade shape the TCR repertoire of tumor-infiltrating T cells. Cancer immunology research, 6(2), 139-150.
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5

Multiparametric Flow Cytometry Analysis

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DCs suspended in FACS buffer (PBS containing 0.5% BSA and 0.05% NaN3) were blocked with 2% mouse serum on ice for 20 min and stained with various combinations of fluorophore-conjugated antibodies against human or mouse DC surface markers on ice for 30 min in the dark. Mouse DCs were stained with FITC-anti-mouse CD86 (clone GL1, TONBO Biosciences, San Diego, CA), PE-anti-mouse CD80 (clone 16-10A1, TONBO), Pacific Blue-anti-mouse CD83 (clone Michel-19, BD), and APC-anti-mouse I-A/E (clone M5/114.15.2, eBioscience). Single LLC tumor cell suspensions were stained with FITC-anti-mouse CD4 (clone GK1.5, Tonbo), PerCP-Cy5-anti-mouse-B220 (clone RA3-6B2, Tonbo), APC-anti-mouse-CD11c (clone HL3, BD), eFluor450-anti-mouse CD45 (clone 30-F11, eBioscience), and APC-Cy7-anti-mouse-CD8 (clone 53 − 6.7, Tonbo). Data of the stained samples were acquired using an LSR II flow cytometer (BD) and analyzed using the software FlowJo.
Liu S., Han Z., Trivett A.L., Lin H., Hannifin S., Yang D, & Oppenheim J.J. (2019). Cryptotanshinone has curative dual anti-proliferative and immunotherapeutic effects on mouse Lewis lung carcinoma. Cancer Immunology, Immunotherapy, 68(7), 1059-1071.
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