Mouse monoclonal anti plk1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Mouse monoclonal anti-PLK1 is a laboratory reagent that can be used to detect and analyze the presence of the Polo-like kinase 1 (PLK1) protein in various experimental systems. PLK1 is a serine/threonine-protein kinase that plays a critical role in cell division and is involved in the regulation of various cellular processes.

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Lab products found in correlation

Mouse monoclonal anti cyclin b1, by Santa Cruz Biotechnology (2 mentions) Mouse monoclonal anti β actin, by Merck Group (2 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti p53, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti vinculin, by Santa Cruz Biotechnology (1 mentions) Rabbit polyclonal anti bcl xl, by Santa Cruz Biotechnology (1 mentions) Rabbit polyclonal anti akt, by Cell Signaling Technology (1 mentions) Paclitaxel t7402, by Merck Group (1 mentions) Rabbit polyclonal anti p21, by Santa Cruz Biotechnology (1 mentions) Pe annexin v 556421 and 7aad 21 68981e, by BD (1 mentions) Rnase a, by Qiagen (1 mentions) Mouse monoclonal anti hec1, by Abcam (1 mentions)

2 protocols using mouse monoclonal anti plk1

Cell Lysis and Western Blot Analysis

Cited 1 time

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Cell lysis and Western blot analysis were performed as described previously [40 (link)], using the following antibodies: Rabbit polyclonal anti-SIK2 (Cell Signaling, Danvers, MA, USA), rabbit polyclonal anti-SIK2-pS385 (Kinexus, Sydney, Australia), rabbit polyclonal anti-AKT (Cell Signaling), rabbit polyclonal anti-AKT-pS473 (Cell Signaling), mouse monoclonal anti-β-actin (Sigma-Aldrich), rabbit polyclonal-anti KIF18B (ThermoFisher scientific, Waltham, MA, USA), rabbit polyclonal anti-EB1 (Abcam, Cambridge, UK), mouse monoclonal anti-cyclin B1 (Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal anti-Histone 3-pS10 (ThermoFisher scientific), mouse monoclonal anti-PLK1 (Santa Cruz Biotechnology), mouse monoclonal anti-vinculin (Santa Cruz Biotech), mouse monoclonal anti-P53 (Santa Cruz Biotech), rabbit polyclonal anti-P21 (cell signaling), rabbit polyclonal anti-BCL-xL (Santa Cruz Biotechnology), rabbit polyclonal anti-Cyclin D (Santa Cruz Biotechnology).
Reagents were purchased from the following sources: Paclitaxel (T7402) Sigma-Aldrich, propidium iodide (440300250) Acros Organics (Fair Lawn, NJ, USA), RNase A (1007885) Qiagen (Hilden, Germany), PE Annexin V (556421) and 7AAD (21-68981E) BD Biosciences, Caspase-Glo^® 3/7 Assay system (P1781) Promega, RO3306 (S7747) Selleckchem (Houston, TX, USA).

Raab M., Rak M., Tesch R., Gasimli K., Becker S., Knapp S., Strebhardt K, & Sanhaji M. (2021). The Small-Molecule Inhibitor MRIA9 Reveals Novel Insights into the Cell Cycle Roles of SIK2 in Ovarian Cancer Cells. Cancers, 13(15), 3658.

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Western Blot Analysis of Cell Cycle Markers

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Western blot analysis was performed as described [47 (link)-49 (link)] and the following primary antibodies were used: mouse monoclonal anti-Kif2c (Santa Cruz biotechnology, Heidelberg), mouse monoclonal anti-Plk1 (Santa Cruz biotechnology), mouse monoclonal anti-cyclin B1 (Santa Cruz biotechnology), mouse monoclonal anti-β-actin (Sigma-Aldrich) and mouse monoclonal anti-Hec1 (Abcam, Darmstadt). Cell cycle was analyzed as described [17 (link),19 (link)].

Ritter A., Sanhaji M., Steinhäuser K., Roth S., Louwen F, & Yuan J. (2014). The activity regulation of the mitotic centromere-associated kinesin by Polo-like kinase 1. Oncotarget, 6(9), 6641-6655.

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