The hESC lines in this study are as follows: UCLA1 (46, XX), UCLA2 (46, XY)(Diaz Perez et al., 2012 (link)), UCLA8 (46, XX)(Chen et al., 2017 (link)), and H1 OCT4-GFP (H1) (46, XY)(Gkountela et al., 2015 (link)). All hESCs were cultured on mitomycin C-inactivated mouse embryonic fibroblasts (MEFs) and split every 7 days using Collagenase type IV (GIBCO, 17104–019). hESC media was comprised of 20% knockout serum replacement (KSR) (GIBCO, 10828–028), 100mM L-Glutamine (GIBCO,25030–081), 1x MEM Non-Essential Amino Acids (NEAA) (GIBCO, 11140–050), 55mM 2-Mercaptoethanol (GIBCO, 21985–023), 10ng/mL recombinant human FGF basic (Proteintech HZ1285), 1x Penicillin-Streptomycin (GIBCO, 15140–122), and 50ng/mL primocin (InvivoGen, ant-pm-2) in DMEM/F12 media (GIBCO, 11330–032). All hESC lines used in this study are registered with the National Institute of Health Human Embryonic Stem Cell Registry and are available for research use with NIH funds. hESCs used in this study were routinely tested for mycoplasma (Lonza, LT07–418). All experiments were approved by the UCLA Embryonic Stem Cell Research Oversight Committee.
Recombinant human fgf basic
Manufactured by Proteintech
Recombinant human FGF basic is a protein that belongs to the fibroblast growth factor (FGF) family. It is produced using recombinant DNA technology. FGF basic is a signaling molecule that plays a role in various cellular processes, including cell proliferation, differentiation, and angiogenesis.
Automatically generated - may contain errors
Lab products found in correlation
Ant pm 2, by Thermo Fisher Scientific (2 mentions)
Collagenase type 4, by Thermo Fisher Scientific (2 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (2 mentions)
Lt07 418, by Lonza (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Primocin, by InvivoGen (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
2 protocols using recombinant human fgf basic
1
Characterization of hESC Lines for Research
2
Culturing Primed Human Embryonic Stem Cells
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UCLA2 and UCLA1 hESC are cultured as previously described16 (link), briefly the hESCs are cultured in hESC media, which was composed of 20% knockout serum replacement (KSR) (Life Technologies, A3181502), 1x MEM Non-Essential Amino Acids (NEAA) (Fisher Scientific, 25-025-CI), 1x Penicillin/Streptomycin/Glutamine (Thermo Fisher, 10378016), 55 µM 2- Mercaptoethanol (Life Technologies, 21985-023), 10 ng/mL recombinant human FGF basic (Proteintech, HZ1285), and 50 ng/mL primocin (InvivoGen, ant-pm-2) in DMEM/F12 media (GIBCO, 11330-032). The primed hESCs were split by 1 mg/ml Collagenase type IV (GIBCO, 17104-019) and maintained routinely on mitomycin C (MMC)-inactivated mouse embryonic fibroblasts (MEFs). The hESCs were split every 7 days using Collagenase type IV (GIBCO, 17104-019). HEK293 cells were acquired from ATCC (Cat# CRL-3216). No lines used in this study belong to the International Cell Line Authentication Committee register of misidentified cell lines.
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