The miR-18a mimic, inhibitor, and negative control miRNA were purchased from RiboBio (Guangzhou China) and transfected into cells at 100 nM concentrations via Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. KLF4 plasmid (1ug; Origene, Rockville, MD, USA) was also transfected into cells in the presence or absence of the miR-18a mimic by Lipofectamine 2000. After transfection twice in 48 h, cells were used in the subsequent experiments. RT-PCR and Western blotting were used to evaluate the transfection efficacy.
Mir 18a mimic
Manufactured by RiboBio
Sourced in China
MiR-18a mimic is a synthetic RNA molecule designed to mimic the function of the naturally occurring microRNA miR-18a. MicroRNAs are small, non-coding RNA molecules that play a crucial role in gene expression regulation. The MiR-18a mimic is a laboratory tool used for research purposes, intended to facilitate the study of miR-18a and its biological functions.
Automatically generated - may contain errors
Lab products found in correlation
Mir 18a inhibitor, by RiboBio (3 mentions)
Mir 18a mimic, by OriGene (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Klf4 plasmid, by OriGene (1 mentions)
Lipofectamine 2000, by OriGene (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Polybrene, by Merck Group (1 mentions)
Rpmi 1640 media, by Corning (1 mentions)
4 protocols using mir 18a mimic
1
Transfection Protocol for miR-18a Mimic and KLF4 Plasmid
2
Lentiviral Overexpression of miR-18a in NPC
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Lentivirus overexpressing miR-18a was purchased from GenePharma (Shanghai, China). NPC cells were infected with recombinant lentivirus transducing units plus 8 mg/ml Polybrene (Sigma, St Louis, Missouri, USA). Stable cell lines were selected using 4 μg/mL puromycin. Transient transfection was performed using Lipofectamine 2000 reagent (Invitrogen, CA, USA) in OPTI-MEM media. miR-18a mimic, miR-18a inhibitor, siRNA against LMP1 or SMG1 and their negative controls were obtained from RiboBio (Guangzhou, China). pcDNA3.1(+)-LMP1 plasmid was kindly provided by Professor BiJun Huang (Sun Yat-sen University Cancer Center).
3
Oligonucleotide Transfection with Lipofectamine
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Lipofectamine 2000 (Invitrogen) was used for oligonucleotide transfection in this study per the manufacturer’s recommendations. The miR-18a mimic, miR-18a inhibitor, and negative control (NC) miRNA were designed and synthesized by RiboBio (Guangzhou, P.R. China). IRF2 or CBX7 expression constructs (1 μg; OriGene, Beijing, P.R. China) were transfected into cells with or without miR-18a mimic. After transfection for 48 h, the cells were collected and analyzed by quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot to evaluate the transfection efficacy.
4
Regulating miR-18a and Bcl2L10 in HCC
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Human HCC cell lines HepG2 and SMMC7721 and normal liver cell line LO2 were purchased commercially from Tianjin Medical University Cancer Institute and Hospital. DMEM or RPMI-1640 media (Corning Incorporated, Corning, NY, USA) containing 10% FBS (Hyclone, Logan, UT, USA) were used to maintain cells. Cells and media were kept in a 5% CO2 environment in a incubator at 37°C. The medium was replaced every 2 days. miR-18a mimic (Ribobio, Guangzhou, China), miR-18a inhibitor (Ribobio), and Bcl2L10-overexpressing plasmids (Genechem, Shanghai, China) were used to transfect HCC cell lines in order to regulate miR-18a and Bcl2L10 expressions.
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