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Cary 660 series ftir

Manufactured by Agilent Technologies
Sourced in United States, Italy

The Cary 660 series FTIR is a Fourier Transform Infrared Spectrometer, a laboratory instrument used for the analysis of chemical compounds. It measures the absorption and emission spectra of solid, liquid, and gaseous samples, providing information about their molecular structure and composition.

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8 protocols using cary 660 series ftir

1

ATR-FTIR Characterization of Formulation Components

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The components of the formulation under study, either alone or in the form of mixtures or granulates, were characterized by ATR-FTIR (Attenuated Total Reflectance-Fourier Transform InfraRed) spectroscopy (Cary 660 series FTIR, Agilent Technologies, Milano, Italy). Before analysis, they were vacuum dried overnight at 37 °C. The spectra were acquired in the 650–4000 cm−1 range, with a resolution range of 4 cm−1 and 64 scans.
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2

FTIR Analysis of SLN Formulation

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The infrared spectra of the samples were obtained using Cary 660 series FTIR (Agilent Technologies, Santa Clara, CA, USA) and MIRacle ATR (attenuated total reflectance) systems. Pure drug, lipid excipients along with their physical mixtures and the final SLN formulation were studied for any interactions.
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3

Thermal and Spectroscopic Analysis of Inserts

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DSC thermograms for pure IN or CIP, PEO N10 and the 10% w/w inserts were collected using a Diamond Differential Scanning Calorimeter (Perkin-Elmer Life and Analytical Sciences) (24 ).The samples were weighed and sealed in aluminum pans and were heated from 0°C to 270°C at a heating rate of 10°C/min under nitrogen purge (20 mL/min). Infrared spectra (IR) for PEO N10, drug/polymer physical mixture, IN / CIP, and melt cast inserts (10% w/w) were obtained using a Cary 660 series FTIR (Agilent technologies) and MIRacle ATR (Attenuated Total reflectance).
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4

Comprehensive Spectroscopic Analysis of Fractions

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Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy was performed (Cary 660 series FT-IR, Agilent Techologies, Santa Clara, CA, USA). RP-CrudeJSP, RP-JSP1 and RP-JSP2 fraction were analyzed in % of absorbance, between 600–4000 cm−1 with resolution of 4 cm−1 and 32 number of scans.
1H NMR spectra were acquired on Bruker Ultrashield™ spectrometer working at 400 MHz for 1H, at 25 ± 0.1 °C on 2%wt, D2O samples.
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5

IR Spectroscopy of SLN and NLC

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The infrared spectra (IR) of the SLN and NLC formulations were obtained using Cary 660 series FTIR (Agilent Technologies) and MIRacle ATR (attenuated total reflectance) systems. The ratios of drug and lipids used in this set of studies were similar to the weight ratios in the IN lipid formulations.
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6

Thermal and Spectroscopic Analysis of Polymeric Films

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DSC thermograms for pure HT, PEO N10 and the 10 %w/w film was collected using a Diamond Differential Scanning Calorimeter (Perkin-Elmer® Life and Analytical Sciences) [33 ]. The samples were weighed and sealed in aluminum pans and were heated from 0 °C to 270 °C at a heating rate of 10 °C/min under nitrogen purge (20 mL/min). Infrared spectra (IR) for PEO N10, HT, and melt-cast film (10 %w/w and 20 %w/w) were obtained using a Cary 660 series FTIR (Agilent Technologies) and MIRacle™ Single Reflection ATR (PIKE Technologies). Further, the IR spectrum of the 13 mm matrix film was collected randomly from different areas of the film, to evaluate distribution uniformity within the polymer matrix.
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7

Attenuated Total Reflectance FTIR Analysis

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Attenuated total reflectance infrared spectroscopy was performed by using Cary 660 series FTIR, Agilent Technologies, Santa Clara, CA USA. The products are analyzed in a range between 650 and 4000 cm−1, with a resolution range of 4 cm−1 and 64 scans.
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8

ATR-FTIR Characterization of BEO/QA-Ch/MCD

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ATR-FTIR spectra of pure BEO, QA-Ch-MCD and the physical mixture BEO/QA-Ch/MCD (ratio 1:1, w/w) were collected at room temperature in the wavenumber range 400–4000 cm−1 with a resolution of 2 cm−1 and 128 scans using a Cary 660 series FTIR (Agilent Technologies, Milan, Italy). The acquired spectra were processed (WaveMetrics, Inc., SW Nimbus, Portland, OR, USA) software. Spectrum subtraction was performed using the 1738 cm−1 band as a reference.
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