Anti slug clone c19g7

The immunoblotting, isolation of nuclear protein fractions, immunoprecipitation and mice protein extraction procedures were performed as previously described [22 (link)].
The primary antibodies used in this work are as follows: human anti-p53 (clone BP53-12, Novocastra Laboratories, Newcastle Upon Tyne, UK), mouse anti-p53 (clone 1C12, Cell Signaling Technology, Beverly, MA, USA), anti-RPL11 (clone 3A4A7, Invitrogen, Carlsbad, UK), anti-PARP-1 (Cell Signaling Technology), anti-RPS6 (clone C-8, Santa Cruz Biotechnology, CA, USA), anti-RPS14 (clone H-130, Santa Cruz Biotechnology), anti-Mdm2 (clone SMP14 and clone H-221, Santa Cruz Biotechnology), anti-Slug (clone C19G7, Cell Signaling Technology), anti-E-cadherin (clone 24E10, Cell Signaling Technology), anti-c-Myc (clone N-262, Santa Cruz Biotechnology), anti-β-actin (clone AC-74, Sigma-Aldrich), and anti-Lamin B (C-20, Santa Cruz Biotechnology). Horseradish peroxidase-conjugated secondary antibodies were from GE-Healthcare (Milan, Italy).

Freshly isolated SCs were cytospun onto slides and fixed with 4% PFA. After being permeabilized by 0.2% Trition X-100, cells were then blocked in PBS with 1% bovine serum albumin (BSA) for 1 h at room temperature followed by staining with anti-Pax7 (DSHB, Iowa, USA, 1:10) and anti-Slug (Clone C19G7, Cell Signaling, USA, 1:400) primary antibodies overnight at 4 °C. Cells were washed three times with PBS and incubated with appropriate secondary antibody for 1 h at room temperature.