Quantikine human endoglin cd105

HUVEC monolayers were scratched with a pipette tip as described above. Culture supernatants were collected at the indicated times and analyzed by ELISA assays. Concentrations of human soluble endoglin and human IL-6 in the cell culture media were determined according to the manufacturer protocol by Quantikine Human Endoglin/CD105 and Quantikine Human IL-6, respectively (DNDG00 and D6050; R&D Systems). Soluble endoglin was also measured in the culture media of HUVECs or HEK293T cells previously transfected with expression vectors, as indicated. All immunoassays were measured in a GloMax multidetection system (Promega) and normalized by the percentage of cells in each control and post-wounded well.

Blood samples were collected from 37 RC and 81 CC patients at time 0 (immediately before surgery) and at different time points (3, 6, and 9 months) after resection of the tumor sample. sEng and human VEGF concentrations in plasma were measured and quantified by ELISA, according to the manufacturer’s protocol by Quantikine Human Endoglin/CD105 and Quantikine Human VEGF, respectively (DNDG00 and DVE00; R&D Systems). All immunoassays were measured with a GloMax multidetection system (Promega).