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Biotin conjugated anti ifn γ

Manufactured by Cellular Technology
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Biotin-conjugated anti–IFN-γ is a laboratory reagent used in immunoassays and other research applications. It consists of an antibody specific to the cytokine interferon-gamma (IFN-γ) that has been conjugated to biotin, a small molecule that can be used to detect and capture the antibody-antigen complex.

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Lab products found in correlation

Anti cd3, by Thermo Fisher Scientific (4 mentions) Immunospot 4.0 analyzer, by Cellular Technology (2 mentions) Mu ifn γ, by Cellular Technology (2 mentions) Anti ifn γ capture antibody, by Cellular Technology (2 mentions) Sars cov 2 s peptide, by Miltenyi Biotec (2 mentions) N peptide pools, by Miltenyi Biotec (2 mentions) Immunospot software, by Cellular Technology (2 mentions) Sars cov 2 s peptide pool, by Miltenyi Biotec (2 mentions) Elispot plate, by Merck Group (1 mentions) Millipore elispot plates, by Merck Group (1 mentions)

4 protocols using biotin conjugated anti ifn γ

1

SARS-CoV-2 Epitope-Specific T-cell Assay

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ELISPOT was performed according to the manufacturer’s instructions (Cellular Technology Ltd.; MU IFN-γ). Plates were coated with anti–IFN-γ capture antibody (Cellular Technology Ltd.) at 4°C overnight. Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S-peptide (2 μg/ml; Miltenyi Biotec, 130–126-701) or N-peptide pools (2 μg/ml; Miltenyi Biotec, 130–126-699) for 24 hours at 37°C. Splenocytes stimulated with anti-CD3 (1 μg/ml; Thermo Fisher Scientific, 16–0031-82) or medium alone were used as positive and negative control, respectively. This was followed by incubation with biotin-conjugated anti–IFN-γ (Cellular Technology Ltd.) for 2 hours at room temperature and then alkaline phosphatase–conjugated streptavidin for 30 min. The plates were washed and scanned using an ImmunoSpot 4.0 analyzer, and the spots were counted with ImmunoSpot software (Cellular Technology Ltd.) to determine SFC per 106 splenocytes.
Hajnik R.L., Plante J.A., Liang Y., Alameh M.G., Tang J., Bonam S.R., Zhong C., Adam A., Scharton D., Rafael G.H., Liu Y., Hazell N.C., Sun J., Soong L., Shi P.Y., Wang T., Walker D.H., Sun J., Weissman D., Weaver S.C., Plante K.S, & Hu H. (2022). Dual spike and nucleocapsid mRNA vaccination confer protection against SARS-CoV-2 Omicron and Delta variants in preclinical models. Science translational medicine, 14(662), eabq1945.
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2

SARS-CoV-2 Spike Peptide ELISPOT Assay

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ELISPOT plates (Millipore Ltd., Burlington, VT, USA) were coated with anti-IFN-γ capture Ab (Cellular Technology Ltd., Shaker Heights, OH, USA) at 4 °C overnight. Splenocytes or lung leukocytes were then stimulated with SARS-CoV-2 S peptide pools (2 μg/mL, Miltenyi Biotec, Auburn, CA, USA) for 24 h at 37 °C. Cells stimulated with anti-CD3 (1 μg/mL, e-Biosciences, San Diego, CA, USA) or medium alone were used as controls. Next, cells were incubated with biotin-conjugated anti-IFN-γ (Cellular Technology Ltd., Shaker Heights, OH, USA) for 2 h at room temperature, and then alkaline phosphatase-conjugated streptavidin (Cellular Technology Ltd., Shaker Heights, OH, USA) for 30 min. After washing, plates were scanned using an ImmunoSpot 6.0 analyzer and analyzed by ImmunoSpot software to determine the spot-forming cells (SFC) per 106 splenocytes or leukocytes.
Singh A., Adam A., Rodriguez L., Peng B.H., Wang B., Xie X., Shi P.Y., Homma K, & Wang T. (2023). Oral Supplementation with AHCC®, a Standardized Extract of Cultured Lentinula edodes Mycelia, Enhances Host Resistance against SARS-CoV-2 Infection. Pathogens, 12(4), 554.
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3

SARS-CoV-2 Epitope-Specific T-cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISPOT was performed according to the manufacturer’s instructions (Cellular Technology Ltd.; MU IFN-γ). Plates were coated with anti–IFN-γ capture antibody (Cellular Technology Ltd.) at 4°C overnight. Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S-peptide (2 μg/ml; Miltenyi Biotec, 130–126-701) or N-peptide pools (2 μg/ml; Miltenyi Biotec, 130–126-699) for 24 hours at 37°C. Splenocytes stimulated with anti-CD3 (1 μg/ml; Thermo Fisher Scientific, 16–0031-82) or medium alone were used as positive and negative control, respectively. This was followed by incubation with biotin-conjugated anti–IFN-γ (Cellular Technology Ltd.) for 2 hours at room temperature and then alkaline phosphatase–conjugated streptavidin for 30 min. The plates were washed and scanned using an ImmunoSpot 4.0 analyzer, and the spots were counted with ImmunoSpot software (Cellular Technology Ltd.) to determine SFC per 106 splenocytes.
Hajnik R.L., Plante J.A., Liang Y., Alameh M.G., Tang J., Bonam S.R., Zhong C., Adam A., Scharton D., Rafael G.H., Liu Y., Hazell N.C., Sun J., Soong L., Shi P.Y., Wang T., Walker D.H., Sun J., Weissman D., Weaver S.C., Plante K.S, & Hu H. (2022). Dual spike and nucleocapsid mRNA vaccination confer protection against SARS-CoV-2 Omicron and Delta variants in preclinical models. Science translational medicine, 14(662), eabq1945.
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4

SARS-CoV-2 Spike Peptide-Specific IFN-γ ELISPOT Assay

Cited 1 time
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As described earlier (24 (link)), Millipore ELISPOT plates (Millipore Ltd.) were coated with anti-mouse IFN-γ capture Ab at 1:100 dilution (Cellular Technology Ltd) at 4°C overnight. Splenocytes were stimulated with SARS-CoV-2 S peptide pools (2 μg/mL, Miltenyi Biotec) for 24 h at 37°C. Cells were stimulated with anti-CD3 (1 μg/mL, e-Biosciences) or medium alone, as controls. This was followed by incubation with biotin-conjugated anti-IFN-γ at 1:100 dilution (Cellular Technology Ltd.) for 2 h at room temperature, followed by incubation with alkaline phosphatase-conjugated streptavidin for 30 min. The plates were washed and scanned using an ImmunoSpot 6.0 analyzer and analyzed by ImmunoSpot software to determine the spot-forming cells (SFCs) per 106 splenocytes.
Singh A., Adam A., A.d.i.t.i., Peng B.H., Yu X., Zou J., Kulkarni V.V., Kan P., Jiang W., Shi P.Y., Samir P., Cisneros I, & Wang T. (2024). A murine model of post-acute neurological sequelae following SARS-CoV-2 variant infection. Frontiers in Immunology, 15, 1384516.
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