Shoot height, root length, and fresh weights were determined 25 DAT (days after treatment) and then the samples were separated into shoot and root and perfectly washed with ddH 2 O to eliminate any foreign material. Samples from each treatment with 4 biological replicates were oven dried at 75 • C for three days and subsequently, the dry weight of the roots and shoots were determined in gram. Later each dried sample was weighed (about 0.2 g), ground, and made into ashes by heating the samples at 550 • C for half a day. Before dilution with ddH 2 O, the ashes were digested in 30% HNO 3 and then Na + and K + concentration were quantified using flame atomic absorption spectrometry (SHIMADZU AA-6300, Columbia, Maryland, USA) [35] (link).
Aa 6300
Manufactured by Shimadzu
Sourced in Japan
The AA-6300 is an atomic absorption spectrometer manufactured by Shimadzu. It is a laboratory instrument used for the quantitative determination of chemical elements using the atomic absorption technique.
Automatically generated - may contain errors
Lab products found in correlation
Gfa ex7i, by Shimadzu (6 mentions)
Spad 502, by Konica Minolta (3 mentions)
Suprapure, by Merck Group (2 mentions)
Hvg 1, by Shimadzu (2 mentions)
K alpha, by Thermo Fisher Scientific (2 mentions)
Ea 3000, by Vector Laboratories (1 mentions)
Fiastar 5000, by Foss (1 mentions)
Vcx130, by Sonics (1 mentions)
M 410, by Corning (1 mentions)
Li 6200, by LI COR (1 mentions)
Mini pam, by Walz (1 mentions)
Titan mps, by PerkinElmer (1 mentions)
Uv 4802h, by Unico (1 mentions)
Ccm 200 plus, by Opti-Sciences (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Dry thermo unit, by TAITEC (1 mentions)
Multiskan spectrum microplate spectrophotometer, by Agilent Technologies (1 mentions)
Vario micro cube, by Elementar (1 mentions)
Whatman filter paper, by Cytiva (1 mentions)
Atomic absorption spectrophotometer, by Shimadzu (1 mentions)
97 protocols using aa 6300
1
Quantifying plant growth and mineral content
2
Quantifying Zn2+ in Tetraselmis marina
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To measure Zn2+ in living and non-living biomass of the Tetraselmis marina AC16-MESO microalgae, 0.5 and 1 g of microalgal biomass sample (in triplicate) was homogenized using an agate mortar in a reflux system with a glass funnel covered with a watch glass. The pieces were placed in a 125 mL beaker for disintegration with 10 mL of HNO3 Suprapur and heated on a heating plate at 150 °C for 2 h. Subsequently, the resulting solution was filtered (0.45 µm) and packed into a 25 mL volumetric flask with deionized water. The quantification of metals was performed by atomic absorption spectrophotometry (Shimadzu, AA 6300) by using the flame technique at a wavelength of 213.9 nm with a mixture of C2H2 gas. The concentrations were expressed as mg g−1 wet weight [47 (link)].
3
Mineral Content Analysis of Bread
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The mineral composition of the developed bread, specifically its calcium, phosphorus, magnesium, iron, and zinc content, was evaluated using the methodology outlined by Ref. [23 ]. The standard solutions of minerals were prepared in distilled water. These standard and sample solutions were evaluated for mineral content with an atomic absorption spectrophotometer (Model: AA-6300, Shimadzu, ASIA Japan-Korea). The standard curves for absorption against concentration were drawn to calculate the results.
4
Measuring Heavy Metal Accumulation in Plants
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After 15 days of treatment, the SPAD values (chlorophyll meter readings) were determined in the top second fully expanded leaves using a chlorophyll meter (Minolta SPAD-502). Plants were collected and separated into roots and shoots after the plant height (PH) and root length (RL) were measured. Shoot dry weight (SDW) and root dry weight (RDW) was measured, after which the shoots and roots were powdered and ashed at 550 °C for 12 h. The ash was digested with 5 mL 30% HNO3 and then diluted with deionized water. Cd, Mn, and Zn concentrations in shoots (ShCd, ShMn, and ShZn) and roots (RCd, RMn, and RZn) were determined using flame atomic absorption spectrometry (AA6300; Shimadzu, Tokyo, Japan).
5
Analyzing Soil Chemical Properties and Solar Radiation
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The contents of SOC and TN were determined by CN Analyzer (EA 3000, Vector, Italy) using 10 mg of dried powder samples. Soil samples for TP were pretreated by Micro-Kjeldahl digestion (HNO3: HClO4 at 3:1) and determined using a flow injection auto-analyzer (FIA Star 5000, FOSS, Denmark). The NH4+-N and NO3--N were extracted by 50 mL of 1 mol L-1 KCl from 30-g fresh soil samples and determined by a flow injection auto-analyzer (FIA Star 5000, FOSS, Denmark). The AK, ACa, and AMg were extracted by 100 mL of 1 mol L-1 NH4Cl from 0.5 g dried soil samples and determined by an atomic absorption spectrophotometer (AA-6300, Shimadzu, Japan). The solar radiation intensity (SRI) was measured with an illuminance meter (TES-1339, TES, China), and the value was recorded at 2:00 p.m., 30 cm above the top of the B. striata. In this study, soil chemical properties and SRI were regarded as environmental information, and the results were presented in Table 1
6
Mineral Content Analysis of Cricket Powder
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The cricket powder was ashed and digested in 6N HCl and the content of the various minerals (Iron, Zinc, Calcium, magnesium, sodium, potassium, manganese, copper and cobalt) determined using atomic absorption spectrometry (AAS) (Shimadzu, AA-6300, Tokyo, Japan) according to AOAC methods (23 ).
7
Metal Bioaccumulation in Recombinant E. coli
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The metal bioaccumulations in E. coli cells expressing MTs were determined according to the method of Sauge-Merle et al. (2012) . Cultures of the control cells (harboring pET-28a-SUMO) and the three recombinant bacteria (harboring pET28a-SUMO-MT, pET28a-SUMO-2MT and pET28a-SUMO-3MT) were diluted 1:100 in 50 mL LB medium with 50 μg/mL kanamycin. IPTG (0.7mM) and metal ions with the same concentration (300 μM ZnSO 4, CdCl 2 , and CuSO 4 ) were added and cells were cultured for 6h at 37℃. Cells were collected by centrifugation at 6000g for 20 min at 4 ℃. Cell pellets were washed twice in fresh LB medium and dry weight of cells measured after dehydration at 80℃ for 48h. Metal ion accumulations were determined by flame atomic spectrometry (SHIMADZU AA-6300, Japan), measuring Cu at 324.8nm, Cd at 228.8nm and Zn at 213.9nm. Metal ion accumulation was expressed as μmol/g dry weight bacterial cells.
8
Mineral Analysis of Fish Samples
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The preparation of samples for mineral elements analysis followed a method described by AOAC (1995 ). Approximately 5 g of respective raw, fried, and powdered samples were placed in a Teflon digestion vessel and double acid digested with nitric acid (HNO3) and perchloric acid (HCLO4). Samples were then analyzed for mineral contents of iron (Fe), manganese (Mn), zinc (Zn), potassium (K), calcium (Ca) using the atomic absorption spectrophotometer (Shimadzu AAS, AA‐6300). Total phosphorus was determined by spectrophotometric vanadium phosphomolybdate method. The mineral concentration was expressed as mg mineral/kg fish dry weight.
9
Determination of Sodium Content in Barley
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Dry barley roots and shoots were ground and passed through a 2-mm mesh sieve. A 0.3 g weighed sample was digested in 10 mL 98% H2SO4 and 3 mL 30% H2O2 for 5 h, and the Na+ content was determined according to Skoog et al. (2000) by using a flame photometer (AA6300, Shimadzu, Tokyo, Japan).
10
Slug Mucus Metal Analysis
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Mucus samples from three
slugs were collected, each in 500 μL
DW by the process described above. For metal detection, these samples
were pooled together, 1.5 mL of DW was added to it, and incubated
for 2 h. The samples were then sonicated for 2 min at 60% amplitude
and a 10 s pulse rate with an ultrasonicator model VCX130 (with probe
microtip of diameter of 3 mm) from Sonics & Materials Inc. Newtown,
CT, USA, and centrifuged at 13000 rpm for 15 min. The supernatant
was then collected (no pellet observed) and acid digested in 50 mL
of aqua regia. This extract was then subjected to atomic absorption
spectroscopic (AAS) (AA-6300 Shimadzu) analysis for detection of calcium,
magnesium, copper, and zinc. The whole process was carried out in
triplicates.
slugs were collected, each in 500 μL
DW by the process described above. For metal detection, these samples
were pooled together, 1.5 mL of DW was added to it, and incubated
for 2 h. The samples were then sonicated for 2 min at 60% amplitude
and a 10 s pulse rate with an ultrasonicator model VCX130 (with probe
microtip of diameter of 3 mm) from Sonics & Materials Inc. Newtown,
CT, USA, and centrifuged at 13000 rpm for 15 min. The supernatant
was then collected (no pellet observed) and acid digested in 50 mL
of aqua regia. This extract was then subjected to atomic absorption
spectroscopic (AAS) (AA-6300 Shimadzu) analysis for detection of calcium,
magnesium, copper, and zinc. The whole process was carried out in
triplicates.
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