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Phospholipids c

Manufactured by Fujifilm
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Phospholipids C is a specialized lab equipment product manufactured by Fujifilm. It is designed to facilitate the analysis and identification of phospholipids, which are an important class of lipid molecules found in biological systems.

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Lab products found in correlation

Hr series nefa hr 2, by Fujifilm (1 mentions) 14c oleic acid, by PerkinElmer (1 mentions) 14c triolein, by PerkinElmer (1 mentions) Infinity triglyceride, by Thermo Fisher Scientific (1 mentions) Autokit glucose, by Fujifilm (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Qpcr core kit for sybr green 1, by Eurogentec (1 mentions) Infinity cholesterol, by Thermo Fisher Scientific (1 mentions) 3h cholesterol, by PerkinElmer (1 mentions) Omniscript rt, by Qiagen (1 mentions) Poloxamer 407, by Spectrum Chemical (1 mentions) Omniscan, by GE Healthcare (1 mentions) Dspe peg2000, by Merck Group (1 mentions) Superdex 200 increase column, by Cytiva (1 mentions) Dionex ultimate 300, by Thermo Fisher Scientific (1 mentions) Free cholesterol e, by Fujifilm (1 mentions) Powerwave xs, by Agilent Technologies (1 mentions) Chod pap, by Roche (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Gpo pap, by Roche (1 mentions)

Spelling variants of this product

Phospholipid C-test (13 citations) Phospholipid (8 citations) Phospholipids C Test (5 citations) Phospholipid C-test wako (5 citations) Phospholipids C kit (3 citations) Phospholipid C- (2 citations) Phospholipids C-test Wako (2 citations) Free cholesterol E and phospholipids C kits (2 citations)

5 protocols using phospholipids c

1

Comprehensive Lipid Metabolism Profiling

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Infinity Cholesterol (catalog #TR13421), Infinity Triglyceride (catalog #TR22421), and TRIzolTM (catalog #15596018) reagents were purchased from Thermo Fisher Scientific (Middletown, VA, USA). Autokit Glucose (catalog #997-03001), Phospholipids C (catalog #997-01801), and HR Series NEFA-HR(2) (catalog #999-34691, 995-34791, 991-34891, and 993-35191) kits were purchased from Fujifilm Wako Chemicals USA (Richmond, VA, USA). Omniscript RT (catalog #205113) kit was purchased from Qiagen (Germantown, MD, USA) and qPCRTM core kit for SYBR Green I (catalog #10-SN10-05) was from Eurogentec (San Diego, CA, USA). 3H-cholesterol (catalog #NET139001MC), 14C-oleic acid (catalog #NEC317250UC), and 14C-triolein (catalog #NEC674250UC) were from PerkinElmer (Shelton, CT, USA). Poloxamer 407 (catalog #P1166) was purchased from Spectrum Chemical (New Brunswick, NJ, USA). Primary and secondary antibodies were purchased from either Cell Signaling (Danvers, MA, USA) or Abcam (Cambridge, MA, USA). All other chemicals and solvents were obtained from Fisher Scientific through its local distributor in the Kingdom of Saudi Arabia.
Otaibi A.A., Mubarak S.A., Qarni A.A., Hawwari A., Bakillah A, & Iqbal J. (2022). ATP-Binding Cassette Protein ABCC10 Deficiency Prevents Diet-Induced Obesity but Not Atherosclerosis in Mice. International Journal of Molecular Sciences, 23(22), 13813.
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2

Multifunctional Theranostic Nanoparticles

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Distearoyl phosphatidylcholine (DSPC), distearoyl phosphatidylethanolamine methoxy(polyethylene glycol) conjugate (DSPE-PEG2000), cholesterol, and indocyanine green dye (ICG) were purchased from Sigma-Aldrich Co. (St. Louis, MO). scVEGF-PEG3400-DSPE conjugate was prepared by site-specific conjugation of DSPE-PEG3400-maleimide (Nektar Therapeutics) to [C4]-monothiol-scVEGF (SibTech, Inc. Brookfield, CT), using a four-fold molar excess of DSPE-PEG3400-maleimide and 10 min incubation at room temperature. Phospholipids C was purchased from Wako Diagnostics (Richmond, VA). Omniscan™ (GE Healthcare, gadodiamide injection) was used as a T1 contrast agent, and Feridex I.V.® (Bayer HealthCare Pharmaceuticals Inc., ferumoxides injectable solution) and nanomag®-D-spio (micromod Partikeltechnologie GmbH, Rostock, Germany) were used as T2 contrast agents. All other solvents or chemicals were of reagent grade.
Kato Y., Zhu W., Backer M.V., Neoh C.C., Hapuarachchige S., Sarkar S.K., Backer J.M, & Artemov D. (2015). Noninvasive imaging of liposomal delivery of superparamagnetic iron oxide nanoparticles to orthotopic human breast tumor in mice. Pharmaceutical research, 32(11), 3746-3755.
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3

Lipid Extraction and Quantification

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Lipids were extracted from a section of liver at 4 °C overnight in 20 × 2:1 chloroform:methanol solution. An equal volume of phosphate-buffered saline (PBS) was added to the chloroform:methanol and mixed vigorously for 2 min and then spun for 15 min at 4 °C at 3000 rpm in a Sorvall™ Legend™ XTR Centrifuge TX-1000 (Thermo Scientific). The bottom layer containing the lipids in chloroform was extracted, and the chloroform was left to evaporate in the fume hood. The lipids were re-suspended in 1 mL of Isopropanol 10% Triton X-100. Cholesterol and triglyceride assays were performed as just described. Phospholipids were measured using a phospholipid-specific enzymatic assay from Wako (Phospholipids C).
McCabe K.M., Hsieh J., Thomas D.G., Molusky M.M., Tascau L., Feranil J.B., Qiang L., Ferrante AW J.r, & Tall A.R. (2020). Antisense oligonucleotide treatment produces a type I interferon response that protects against diet-induced obesity. Molecular Metabolism, 34, 146-156.
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4

Lipoprotein Separation and Analysis

Cited 1 time
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We sterile-filtered the samples after incubation and applied 350 μl to the FLP for fractionation. We achieved separation using high-resolution SEC that consisted of three Superdex 200 increase columns (Cytiva) in-tandem (2 (link)) operating at a flow rate of 0.3 ml/min in STB. We monitored the lipoprotein distribution in real time using a Dionex Ultimate 300 (Thermo Fisher Scientific) equipped with a flow cell integrated between the last Superdex columns and the fraction collector. The Dionex acquired signal by sampling at 2 Hz with an excitation wavelength of 560 nm and an emission wavelength of 583 nm. We obtained the plasma signal at a sensitivity setting of 1 and CSF signal at a sensitivity setting of 8. We eluted samples with five single column volumes of buffer (~120 ml) and collected 1-ml fractions that we sealed and stored at 4°C until ready for analysis. For plasma, we determined phospholipid content enzymatically (Phospholipids C, Wako) on individual fractions as previously described (2 (link)).
Merrill N.J., Davidson W.S., He Y., Díaz Ludovico I., Sarkar S., Berger M.R., McDermott J.E., Van Eldik L.J., Wilcock D.M., Monroe M.E., Kyle J.E., Bruce K.D., Heinecke J.W., Vaisar T., Raber J., Quinn J.F, & Melchior J.T. (2023). Human cerebrospinal fluid contains diverse lipoprotein subspecies enriched in proteins implicated in central nervous system health. Science Advances, 9(35), eadi5571.
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5

Comprehensive HDL Composition Analysis

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HDL chemical composition was measured using commercially available enzymatic kits, in the microplate reader Power Wave XS (BioTek®, Winooski, USA). Total proteins (Pierce™ BCA Protein Assay Kit, Thermo Scientific, Rockford, USA), TC (CHOD-PAP, total cholesterol, Roche Diagnostics® reagents, Mannheim, Germany), FC (Free Cholesterol E, Wako Chemicals, Richmond, USA), PL (Phospholipids C, Wako Chemicals, Richmond, USA), TG (TG, GPO-PAP, Roche Diagnostics® reagents, Mannheim, Germany) and ApoA-I (TINA QUANT APOA1 V2, Roche Diagnostics® reagents, Mannheim, Germany) were measured, while CE was calculated according to the following formula: (TC-FC) x 1.67 [12] . The relative content of ApoA-I (HDL-ApoA-I) or lipids in HDL was calculated based on their proportion to the total mass of HDL, calculated as the sum of FC, PL, TG, CE, and total proteins. HDL molar concentration was estimated based on particle total mass and molecular weight [12] .
de Lima-Junior J.C., Virginio V.W.M., Moura F.A., Bertolami A., Bertolami M., Coelho-Filho O.R., Zanotti I., Nadruz W., de Faria E.C., de Carvalho L.S.F, & Sposito A.C. (2020). Excess weight mediates changes in HDL pool that reduce cholesterol efflux capacity and increase antioxidant activity. Nutrition, metabolism, and cardiovascular diseases : NMCD, 30(2).
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