Ion x3 em ccd camera

Manufactured by Oxford Instruments

The Ion X3 EM CCD camera is a high-performance imaging device designed for scientific and research applications. It features a back-illuminated electron-multiplying CCD (EMCCD) sensor that provides high quantum efficiency and low noise, enabling the capture of sensitive and detailed images. The camera offers a range of technical specifications including a large sensor size, high resolution, and fast readout speeds. However, as a marketing specialist, I am unable to provide a more detailed description without the risk of interpretation or extrapolation beyond the core function of the product. The technical details and intended uses are best presented by our product experts.

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Lab products found in correlation

N storm, by Nikon (2 mentions) Gluox, by Merck Group (2 mentions) Tetraspeck bead, by Thermo Fisher Scientific (2 mentions)

2 protocols using ion x3 em ccd camera

Super-Resolution STORM Imaging Protocol

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STORM imaging was performed as described (Sunwoo et al., 2015 (link)). Briefly, cells were imaged on an N-STORM (Nikon) equipped with 100×/1.4 N.A. λ objective lens, ion X3 EM CCD camera (Andor), and 647 nm, 561 nm, and 405 nm lasers. Imaging buffer contained 147 mM βME and GluOX (Sigma). For 3D STORM imaging, cylindrical lens was inserted to the optical path to introduce astigmatism. 3D Z calibration was performed using 100 nm TetraSpeck beads (Life Technology). N-STORM module in Element software (Nikon) was used to control microscopes, acquire images, and perform 2D and 3D STORM localizations.

Kundu S., Ji F., Sunwoo H., Jain G., Lee J.T., Sadreyev R.I., Dekker J, & Kingston R.E. (2017). Polycomb Repressive Complex 1 generates discrete compacted domains that change during differentiation. Molecular cell, 65(3), 432-446.e5.

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3D STORM Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

STORM imaging was performed as previously described (Sunwoo et al., 2015 (link)) on an N-STORM (Nikon) equipped with 100x/1.4 N.A. λ objective lens, ion X3 EM CCD camera (Andor), and 3 laser lines (647 nm, 561 nm, and 405 nm). A cylindrical lens was inserted into the optical path to introduce astigmatism. Z calibration was performed using 100 nm TetraSpeck beads (Life Technologies). Imaging buffer containing 147 mM βME and GluOX (Sigma) was used to promote blinking and reduce photo-bleaching. Z calibration was performed using 100 nm TetraSpeck beads. N-STORM module in Element software (Nikon) was used to control microscopes, acquire images, and perform 3D STORM localizations.

Colognori D., Sunwoo H., Kriz A., Wang C.Y, & Lee J.T. (2019). Xist deletional analysis reveals a co-dependency between Xist RNA and Polycomb complexes for spreading along the inactive X. Molecular cell, 74(1), 101-117.e10.

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