Analyst 1

Manufactured by Thermo Fisher Scientific

Sourced in United States, Canada, Germany

Analyst 1.5 software is a data analysis tool designed for use with Thermo Fisher Scientific's analytical instruments. The software provides users with functions to collect, process, and analyze data generated from these instruments.

Automatically generated - may contain errors

Lab products found in correlation

Api 3200 qtrap mass, by AB Sciex (7 mentions) Oasis mcx, by Waters Corporation (6 mentions) Zorbax eclipse xdb c18 column, by Agilent Technologies (5 mentions) Ultimate 3000, by Thermo Fisher Scientific (5 mentions) Agilent 1200 hplc system, by Agilent Technologies (4 mentions) 3200 q trap hybrid triple quadrupole linear ion trap mass spectrometer, by Thermo Fisher Scientific (4 mentions) 20ad hplc system, by Shimadzu (4 mentions) 3200 q trap, by Thermo Fisher Scientific (3 mentions) 2h4 sa, by Merck Group (3 mentions) 13c6 iaa, by Cambridge Isotopes (3 mentions) Api 4000, by Thermo Fisher Scientific (3 mentions) Uplc 1 class system, by Waters Corporation (2 mentions) 1260 hplc system, by Agilent Technologies (2 mentions) Ascentis si 3μ, by Merck Group (2 mentions) Api 3200 tandem mass spectrometer, by Thermo Fisher Scientific (2 mentions) Acquity uplc system, by Waters Corporation (2 mentions) Spss 25, by IBM (2 mentions) Agilent 1100 series hplc system, by Agilent Technologies (2 mentions) Multiquant software, by Thermo Fisher Scientific (2 mentions) Agilent 1260 hplc system, by Agilent Technologies (2 mentions)

Close spelling variants of this product

ProcartaPlex Analyst 1.0 software (29 citations) Analyst Software version 1.6 (20 citations) Analyst version 1.4.2 (13 citations) Analyst QS 1.1 (8 citations) Analyst v. 1.4.2 (5 citations) Analyst Software (version 1.4; (4 citations) ProcartaPlex Analyst Software 1.0 (3 citations) ProtocartaPlex Analyst 1.0 software (3 citations) Analyst® software 1.6 (2 citations)

117 protocols using analyst 1

Sphingolipid Profiling in Epidermis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Epidermal strips were harvested and lysed in RIPA buffer followed by extraction of sphingolipids, as previously reported [26 (link),27 (link),28 (link)]. The extracted lipids dried using a vacuum system (Vision, Daejeon, Korea) were re-dissolved in methanol and analyzed by LC-ESI-MS/MS (API 3200 QTRAP mass, AB SCIEX, MA, USA) by the multiple reaction mode (MRM) [27 (link)]. The ceramide MS/MS transitions (m/z) were 510→264 for C14-ceramide, 538→264 for C16-ceramide, 566→264 for C18-ceramide, 594→264 for C20-ceramide, 622→264 for C22-ceramide, 648→264 for C24:1-ceramide, and 650→264 for C24-ceramide. The dihydroceramide MS/MS transitions (m/z) were 512→284 for C14-dihydroceramide, 540→284 for C16-dihydroceramide, 568→284 for C18-dihydroceramide, 596→284 for C20-dihydroceramide, 624→264 for C22-dihydroceramide, 650→284 for C24:1-dihydroceramide, and 652→284 for C24-dihydroceramide. The sphingoid bases MS/MS transitions (m/z) were 286→238 for C17 sphingosine, 366→250 for C17 sphingosine 1-phosphate (S1P) as an internal standard, 300→252 for C18 sphingosine, and 380→264 for C18 S1P. Data were acquired using Analyst 1.5.1 software (Applied Biosystems, Foster City, CA, USA). The levels of each ceramide subspecies were quantitated using calibration curves with the various concentrations of analytes and internal standard ratio and expressed as pmol/g wet weight of epidermis.

Shin K.O., Ha D.H., Kim J.O., Crumrine D.A., Meyer J.M., Wakefield J.S., Lee Y., Kim B., Kim S., Kim H.K., Lee J., Kwon H.H., Park G.H., Lee J.H., Lim J., Park S., Elias P.M., Park K., Yi Y.W, & Cho B.S. (2020). Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis. Cells, 9(3), 680.

+ Open protocol

+ Expand

Quantification of Cellular Sphingolipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the levels of cellular sphingolipids, such as ceramides and S1P, human KC pre-treated with or without NOX (Apocynin [APO], 100 μM) or ROS generation (N-Acetylcysteine [NAC], 1 mM) inhibitors for 30 mins were incubated with DPE (100 μg/mL) for 24 hrs, followed by extraction of sphingolipids as we reported previously [30 (link),31 (link)]. The extracted lipids dried using a vacuum system (Vision, Seoul, Korea) were re-dissolved in methanol and analyzed by LC-ESI-MS/MS (API 3200 QTRAP mass, AB/SCIEX, Framingham, MA, USA) by selective ion monitoring mode. The ceramide MS/MS transitions (m/z) were 510→264 for C14-ceramide, 538→264 for C16-ceramide, 552→264 for C17-ceramide, 566→264 for C18-ceramide, 594→264 for C20-ceramide, 648→264 for C24:1-ceramide, 650→264 for C24-ceramide, 676→264 for C26:1-ceramide, and 678→264 for C26-ceramide, respectively. The S1P MS/MS transitions (m/z) were 366→250 for C17 S1P as an internal standard and 380→264 for C18 sphingosine 1-phosphate, respectively. Data were acquired using Analyst 1.5.1 software (Applied Biosystems, Foster City, CA). Sphingolipid levels are expressed as pmol per mg protein.

Lee H.S., Park H.Y., Kwon S.P., Kim B., Lee Y., Kim S., Shin K.O, & Park K. (2020). NADPH Oxidase-Mediated Activation of Neutral Sphingomyelinase Is Responsible for Diesel Particulate Extract-Induced Keratinocyte Apoptosis. International Journal of Molecular Sciences, 21(3), 1001.

+ Open protocol

+ Expand

Quantification of Cellular Ceramides

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the levels of cellular ceramides, human HaCaT KC were pretreated with IL-4 (50 ng/mL) for 20 h, followed by incubation with exogenous PS (25 μM) with or without 10 μM of CB1 inhibitor (AM-251, Tocris Bioscience, Ellisville, MO, USA) for 4 h. Extraction of ceramides was performed as we have reported previously [23 (link)]. The extracted lipids were dried using a vacuum system (Vision, Seoul, Korea), re-dissolved in methanol, and analyzed by LC-ESI-MS/MS (API 3200 QTRAP mass, AB/SCIEX, Framingham, MA, USA) in the multiple reaction monitoring (MRM) mode. The ceramide MS/MS transitions (m/z) were 510→264 for C14-ceramide, 538→264 for C16-ceramide, 552→264 for C17-ceramide, 566→264 for C18-ceramide, 594→264 for C20-ceramide, 648→264 for C24:1-ceramide, 650→264 for C24-ceramide, 676→264 for C26:1-ceramide, and 678→264 for C26-ceramide, respectively. Data were acquired using the Analyst 1.5.1 software (Applied Biosystems, Foster City, CA, USA). Ceramide levels are expressed in pmol per mg protein.

Shin K.O., Kim S., Park B.D., Uchida Y, & Park K. (2021). N-Palmitoyl Serinol Stimulates Ceramide Production through a CB1-Dependent Mechanism in In Vitro Model of Skin Inflammation. International Journal of Molecular Sciences, 22(15), 8302.

+ Open protocol

+ Expand

Quantitative LC-MS/MS Analysis of Plasma and Brain Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

All plasma and tissue samples from the PK studies were analyzed with an electrospray ionization LC/MS/MS system [HPLC, Shimadzu, Kyoto, Japan; QTrap 5500, Applied Biosystems, Foster City, CA] described previously(16 (link)). To 10 μl samples of plasma, normal brain or brain tumor homogenate [20% w/w tissue/water], 40 μl of cold acetonitrile was used to precipitate proteins followed by centrifugation at 15000 rpm for 5 min. For both plasma and brain samples, 10 μl aliquots of the resultant supernatant were injected into the LC/MS/MS system. Data collection and analysis were performed using Analyst 1.5.1 software [Applied Biosystems MDS Sciex, Ontario, Canada].

Zhang X., Lv H., Zhou Q., Elkholi R., Chipuk J.E., Reddy M.V., Reddy E.P, & Gallo J.M. (2014). Preclinical Pharmacological Evaluation of a Novel Multiple Kinase Inhibitor, ON123300, in Brain Tumor Models. Molecular cancer therapeutics, 13(5), 1105-1116.

+ Open protocol

+ Expand

Analyzing Macrophage Tryptophan Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols

We cultured 7 × 10⁶ BMDMs in 10-cm dishes overnight in macrophage growth medium to ~90% confluence. The following day, the medium was replaced with cRPMI 6 h prior to stimulation with IL-4 (10 ng ml⁻¹) or LPS (100 ng ml⁻¹) for 24 h. Cells were washed twice with cold PBS, and gently scraped off, pelleted, flash frozen and shipped to Y.-M.L.’s laboratory on dry ice. The extracted metabolites were analysed by LC–electrospray ionization–tandem MS (LC–ESI–MS/MS) (API 5000 QTRAP mass, AB/SCIEX) by MRM mode. Tryptophan and metabolite MS/MS transitions (m/z) were 205 → 188 for tryptophan, 209 → 192 for kynurenine, 225 → 208 for 3-hydroxy-l-kynurenine, 154 → 136 for 3-hydroxyanthranilic acid, 206 → 160 for kynurenic acid, 190 → 144 for xanthurenic acid, 138 → 120 for anthranilic acid, 124 → 106 for 2-picolinic acid, 168 → 150 for quinolinic acid and 199 → 112 for l-mimosine as an internal standard. The metabolites were separated on a Luna C18 column (2.1 × 150 mm, 5.0 μm) with an injection volume of 5 μl and flow rate of 0.3 ml min⁻¹ using 0.1% trifluoroacetic acid for mobile phase A and acetonitrile for mobile phase B. The gradient was as follows: 0 min, 2% B; 12 min, 60% B; 13 min, 60% B; 14 min, 2% B; 20 min, 2% B. Data were acquired using Analyst 1.5.1 software (Applied Biosystems), and are presented as pmol per mg protein.

Covarrubias A.J., Kale A., Perrone R., Lopez-Dominguez J.A., Pisco A.O., Kasler H.G., Schmidt M.S., Heckenbach I., Kwok R., Wiley C.D., Wong H.S., Gibbs E., Iyer S.S., Basisty N., Wu Q., Kim I.J., Silva E., Vitangcol K., Shin K.O., Lee Y.M., Riley R., Ben-Sahra I., Ott M., Schilling B., Scheibye-Knudsen M., Ishihara K., Quake S.R., Newman J., Brenner C., Campisi J, & Verdin E. (2020). Senescent cells promote tissue NAD+ decline during ageing via the activation of CD38+ macrophages. Nature metabolism, 2(11), 1265-1283.

+ Open protocol

+ Expand

Characterization of MβCD Molecules

Check if the same lab product or an alternative is used in the 5 most similar protocols

To identify the average molecular weight and levels of methylation in side chains of MβCD molecules, we carried out the MS analysis. Initially, 0.4 mg of MβCD powder was dissolved in 3 mL of deionized Millipore (Sigma-Aldrich, St. Louis, MO) water as the stock solution (100 μM). An aliquot of the MβCD stock solution was further diluted with 90% methanol to 10 μg/mL (8 μM) for MS analysis. The diluted MβCD solution was directly infused into the API-4000 Mass Spectrometer (Applied Biosystems, Forster City, CA) at a rate of 10 μL/min. The mass spectrometer was used for Q1 scan with positive ion electrospray mode at 5000V and 100V declustering potential. The MS scan range was adjusted from m/z 1,200 to 1,450 at a scan rate of 2 s. The data analysis was performed by Analyst 1.5.1 software (Applied Biosystems, Foster City, CA).

Li R., Hao J., Fujiwara H., Xu M., Yang S., Dai S., Long Y., Swaroop M., Li C., Vu M., Marugan J.J., Ory D.S, & Zheng W. (2017). Analytical Characterization of Methyl-β-Cyclodextrin for Pharmacological Activity to Reduce Lysosomal Cholesterol Accumulation in Niemann-Pick Disease Type C1 Cells. Assay and Drug Development Technologies, 15(4), 154-166.

+ Open protocol

+ Expand

Quantification of Skin and Brain Sphingolipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Skin or brain biopsies from both VPA- and vehicle-treated mice were taken for sphingolipid quantifications after anesthetization with 2% isoflurane in a combination of nitrous oxide and oxygen (7:3, v/v) via an isoflurane vaporizer (VetEquip, Livermore, CA, USA). The levels of ceramide (Cer) and sphingomyelin (SM) were quantified using the LC–ESI–MS/MS (API 3200 QTRAP mass, AB/SCIEX) by selective ion monitoring mode, as described previously [70 (link)–72 (link)]. The MS/MS transitions of ceramides depending on their acyl chain length were 510 → 264 for C14-ceramide, 538 → 264 for C16-ceramide, 552 → 264 for C17-ceramide, 566 → 264 for C18-ceramide, 594 → 264 for C20-ceramide, 648 → 264 for C24:1-ceramide, and 650 → 264 for C24-ceramide, respectively. In addition, the sphingomyelin MS/MS transitions were 718 → 184 for C17 SM (d18:1/17:0) as an internal standard, 704 → 184 for C16 SM, 732 → 184 for C18 SM, 760 → 184 for C20 SM, 788 → 184 for C22 SM, 814 → 184 for C24:1 SM and 816 → 184 for C24 SM, respectively. Data were acquired using Analyst 1.5.1 software (Applied Biosystems, Foster City, CA). The results are expressed as pmol/mg protein.

Shin K.O., Crumrine D.A., Kim S., Lee Y., Kim B., Abuabara K., Park C., Uchida Y., Wakefield J.S., Meyer J.M., Jeong S., Park B.D., Park K, & Elias P.M. (2021). Phenotypic overlap between atopic dermatitis and autism. BMC Neuroscience, 22, 43.

+ Open protocol

+ Expand

Quantification of Sphingosine Kinase and Lyase

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Activities of sphingosine kinase (Sphk) 1 or S1P lyase (S1PL) were assessed as described previously [29 (link),30 (link),31 (link)]. Briefly, lipid extractions were performed by the addition of 100 pmol C17-sphinganine-1-phosphate or 100 pmol of (2E)-d5-hexadecenal, respectively. The extracted lipids were dried using a vacuum system (Vision), to then be re-dissolved in methanol and analyzed by an LC-ESI-MS/MS (AB SCIEX), as described previously [31 (link),32 (link)]. Data were acquired using Analyst 1.5.1 software (Applied Biosystems, Foster City, CA, USA). The activity of Sphk1 or S1PL was expressed as S1P or pentadecanal, respectively (pmol per mg of protein per min).

+ Open protocol

+ Expand

Quantification of Ceramide and S1P

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were treated with or without NOX inhibitor (Apocynin [APO], 100 μM) or ROS generation (N-Acetylcysteine [NAC], 1 mM) for 30 min and then further incubated with DPM for 24 h, followed by extraction of lipids as we reported previously [26 (link),27 (link)]. Ceramide, and S1P were quantitated using LC-ESI-MS/MS (API 3200 QTRAP mass), as we described previously [26 (link),27 (link)]. The ceramide MS/MS transitions (m/z) were 510→264 for C14-ceramide, 538→264 for C16-ceramide, 552→264 for C17-ceramide, 566→264 for C18-ceramide, 594→264 for C20-ceramide, 648→264 for C24:1-ceramide, 650→264 for C24-ceramide, 676→264 for C26:1-ceramide, and 678→264 for C26-ceramide, respectively. The S1P MS/MS transitions (m/z) were 366→250 for C17 S1P as an internal standard and 380→264 for C18 sphingosine 1-phosphate, respectively. Data were acquired using Analyst 1.5.1 software (Applied Biosystems). Sphingolipid levels are expressed as pmol per mg protein.

Shin K.O., Kim S., Kim B., Park H.Y., Jung E., Kim G., Kim D., Cho H.E., Uchida Y, & Park K. (2022). Euphorbia supina Extracts Block NADPH Oxidase-Mediated, Ceramide-Induced Apoptosis Initiated by Diesel Particulate Matter. Pharmaceuticals, 15(4), 431.

+ Open protocol

+ Expand

Quantitative Bioanalysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromatographic separation was performed using Shimadzu Nexera X2 (Shimadzu, Japan) equipped with an autosampler, a column heater, and a binary pump. Utilizing the Cadenza CD-C18 column (150 × 3.0 mm, 3 µm) (Imtakt, Japan) at 40℃, the analytes were separated using the isocratic mobile phase of 10 mM ammonium acetate:100% methanol (5:95, v/v) at a flow rate of 0.4 mL/min. The column was eluted into the Applied Biosystems MDS SCIEX API 4000 triple quadrupole mass spectrometer (Applied Biosystems, Canada) with an ESI source, operating in the multiple reaction monitoring (MRM) mode under unit mass resolution conditions in mass analyzers. The optimized MS condition was as follows: curtain gas flow 20 L/h, collision gas flow 6 L/h, collision energy 45 V, and declustering potential 51 V. Data analysis was performed using Analyst 1.6 software (Applied Biosystems, Canada).

Kim D.H., Cho J.Y., Chae S.I., Kang B.K., An T.G., Shim W.S., Noh Y.S., Hwang S.J., Chung E.K, & Lee K.T. (2017). Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study. Translational and Clinical Pharmacology, 25(4), 173-178.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!