Cy5.5 nhs dye

Manufactured by Lumiprobe

Sourced in Uruguay

Cy5.5 NHS dye is a fluorescent labeling reagent commonly used in various biological and biochemical applications. It is designed to covalently attach to primary amine groups, allowing the labeling of proteins, peptides, and other biomolecules. The dye has an absorption maximum at 675 nm and an emission maximum at 694 nm, making it suitable for detection in the far-red region of the visible spectrum.

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Lab products found in correlation

Cy3 azide, by Lumiprobe (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Paclitaxel ptx, by LC Laboratories (1 mentions) Stannous octoate, by MP Biomedicals (1 mentions) Sephadex g 25 pd 10 column, by GE Healthcare (1 mentions)

2 protocols using cy5.5 nhs dye

Synthesis of Paclitaxel-Loaded Polymeric Micelles

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Methoxy poly(ethylene oxide) (PEO) (M_wt 5000 Da), sodium dodecyl sulfate, and L-Ascorbic
acid (99%) were purchased from Sigma (St Louis, MO). ε-Caprolactone
was purchased from Lancaster synthesis (U.K.). α-Benzylcarboxylate-ε-caprolactone
(BCL) and α-propargyl-carboxylate-ε-caprolactone were
synthesized by Alberta Research Chemicals Inc. (ARCI, Edmonton, AB,
Canada) based on methods published previously by our group.^{39 (link)} 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinimidyl (polyethylene glycol)] (DSPE-PEG3400-NHS)
and methoxy poly(ethylene oxide) (M_wt 2000
Da), covalently linked to distearoyphosphadidyl ethanolamine (mPEO2000-DSPE),
were purchased from Nanocs Inc. Paclitaxel (PTX) (purity> 99.5)
was
purchased from LC Laboratories (Woburn, MA). Rituximab (anti CD20
antibody) and Taxol were provided by Cross Cancer Institute. Stannous
octoate was purchased from MP Biomedicals Inc. (Germany). Spectra/por
dialysis tubing (MWCO, 3.5kDa) was purchased from Spectrum Laboratories
(Rancho Dominguez, CA). Cy3 azide, Cy5.5 NHS dye, and Cu(II) (Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine) (TBTA) complex were purchased
from Lumiprobe (Hallandale Beach, Florida). All other chemicals and
reagents used were of analytical grade.

Saqr A., Vakili M.R., Huang Y.H., Lai R, & Lavasanifar A. (2019). Development of Traceable Rituximab-Modified PEO-Polyester Micelles by Postinsertion of PEG-phospholipids for Targeting of B-cell Lymphoma. ACS Omega, 4(20), 18867-18879.

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Nanoparticle Fluorescent Labeling Protocol

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After amination and sonication, the nanoparticles were conjugated with a Cy5.5-NHS dye (Lumiprobe) to allow for fluorescent/photoacoustic imaging. Briefly, 100 μL of the dye (10 mM stock) suspended in DMSO was mixed with 400 μL of a sodium citrate buffer (20 mM Na-Citrate, 150 mM NaCl, pH 8.4) to dissolve fully. Next, 1.1 mL of the freshly prepared nanoparticles was added to this mixture and placed on a rotor overnight at room temperature. After the conjugation was complete, unconjugated dye was removed with a Sephadex G-25 PD-10 column (GE Healthcare) against the sodium citrate buffer pH 8.4.

Robertson N., Sempere L., Kenyon E., Mallet C., Smith K., Hix J., Halim A., Fan J, & Moore A. (2022). Omniparticle contrast agent for multimodal imaging: synthesis and characterization in an animal model. Molecular imaging and biology, 25(2), 401-412.

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