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Isocage

Manufactured by Tecniplast
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Sourced in Italy, United States

Isocages are a line of ventilated animal enclosures designed for laboratory use. They provide a controlled environment for housing small laboratory animals, such as rodents, while maintaining proper air circulation and filtration. The core function of Isocages is to create a safe and hygienic housing solution for research subjects.

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Lab products found in correlation

Serum separator tube, by BD (2 mentions) Wild type c57bl 6 mice, by Charles River Laboratories (1 mentions) Balb c mice, by Japan SLC (1 mentions) Mesocricetus auratus, by Janvier Labs (1 mentions)

Close spelling variants of this product

Sterile ISOcages HEPA® filtered isocages Isocages bioexclusion system IsoCage N Bio-containment System Isocage system ISOcage P - Bioexclusion System ISOcage P ISOcage biocontainment isolator cages IsoCage N IsoCage bioexclusion systems

17 protocols using isocage

1

Murine Model for Prenatal Studies

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Pregnant C57BL/6 wild type (WT) mice were purchased from the Beijing Vital River Laboratory Animal Technology (Beijing, China) at 12 weeks of age. The mice were kept in level-3 isolators (Isocage, Tecniplast, Italy) in an animal biosafety level 3 (ABSL-3) facility at the Wuhan Institute of Virology (WIV), Chinese Academy of Sciences (CAS), according to the Regulations for the Administration of Affairs Concerning Experimental Animals in China (1988) and the Guidelines for Animal Care and Use, WIV, CAS. New-born mice were housed with the mother until they were 21 days old.
Zhang Y., Yan H., Li X., Zhou D., Zhong M., Yang J., Zhao B., Fan X., Fan J., Shu J., Lu M., Jin X., Zhang E, & Yan H. (2022). A high-dose inoculum size results in persistent viral infection and arthritis in mice infected with chikungunya virus. PLoS Neglected Tropical Diseases, 16(1), e0010149.
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2

IFNAR-/- Mice Maintained in SPF

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IFNAR-/- mice [38 (link)] backcrossed more than 20-fold on the C57BL/6 background were bred under specified-pathogen-free (SPF) conditions, housed in isolated cage units (IsoCage, Tecniplast, Hohenpeißenberg, Germany) and had access to food and water ad libitum. All experiments were approved by the Government of Upper Bavaria, Munich Germany and were performed in compliance with the German Animal Welfare Act.
Forster D., Schwarz J.H., Brosinski K., Kalinke U., Sutter G, & Volz A. (2020). Obstetric Ultrasonography to Detect Fetal Abnormalities in a Mouse Model for Zika Virus Infection. Viruses, 12(1), 72.
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3

Flea-Mediated Plague Transmission in Mice

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At 72 h p.i., pools of 10 potentially infectious fleas were placed in a feeding capsule and attached, using a beeswax/rosin mixture, to the dorsal side of an anaesthetized naïve SKH-1 mouse (50–75 mg ketamine kg−1/0.5–1.0 mg dexmedetomidine kg−1, intaperitoneally). After 1 h, fleas were removed from the capsule using a mechanical aspirator and examined using light microscopy to determine feeding success. Following exposure to potentially infectious fleas, anaesthetic was reversed using atipamezole (5 mg kg−1, intaperitoneally) and mice were placed individually into HEPA-filtered ISOcages (Tecniplast ISOcage). Mice were monitored daily and euthanized when signs of infection with Y. pestis were evident (e.g. slow response to stimuli, hunched posture). Infection was confirmed as described above. Mice not exhibiting signs of infection were held for 22 days p.i., at which time mice were euthanized and blood was collected for serology. Serological evidence of exposure to Y. pestis was determined using passive haemagglutination and inhibition tests (Chu, 2000 ). Titres of ≥ 1 : 10 were considered positive and indicative of Y. pestis transmission from flea to mouse (Chu, 2000 ).
Johnson T.L., Hinnebusch B.J., Boegler K.A., Graham C.B., MacMillan K., Montenieri J.A., Bearden S.W., Gage K.L, & Eisen R.J. (2014). Yersinia murine toxin is not required for early-phase transmission of Yersinia pestis by Oropsylla montana (Siphonaptera: Ceratophyllidae) or Xenopsylla cheopis (Siphonaptera: Pulicidae). Microbiology, 160(Pt 11), 2517-2525.
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4

Germ-Free Mouse Housing and Care

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Adult Swiss Webster GF and CC mice were purchased from Taconic Biosciences (Germantown, NY, USA). All mice were housed in our GF facility in an isolated, ventilated caging system (Isocage, Techniplast, Buguggiate VA, Italy). Mice were maintained on a 12:12 light-dark cycle with ad libitum access to autoclaved food and water. All animal procedures were approved by Georgia State University’s Institutional Animal Care and Use Committee (protocol #A20013) and followed the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
Castillo-Ruiz A., Gars A., Sturgeon H., Ronczkowski N.M., Pyaram D.N., Dauriat C.J., Chassaing B, & Forger N.G. (2023). Brain effects of gestating germ-free persist in mouse neonates despite acquisition of a microbiota at birth. Frontiers in Neuroscience, 17, 1130347.
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5

Establishing Altered Schaedler Flora in Mice

Cited 1 time
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C57BL/6 WT germ free mice were inoculated with ASF feces purchased from ASF Taconic, Inc. (Hudson, NY), which contained the 8 ASF strains, to established Altered Schaedler Flora (ASF) mice as previously described [38 (link)]. Eight-week-old ASF male mice were fed with autoclaved chow and irradiated WSD for 1 week before infecting with 4×108 CFU/per mouse C. rodentium strain ICC180, and placed in isolated ventilated caging system (Isocage, Techniplast, Buguggiate VA, Italy) that prevents exogenous bacterial contamination. Fecal samples were collected at indicated days post infection, and the number of C. rodentium in feces was counted according to described above. After 35 days, the mice were then removed from Isocages and transferred to ABSL2 unit, immediately orally administered 200 μl of the fecal suspension which generated from feces of conventional raised mice, and maintained in a sterile condition (autoclaved cage, food and water).
An J., Zhao X., Wang Y., Noriega J., Gewirtz A.T, & Zou J. (2021). Western-style diet impedes colonization and clearance of Citrobacter rodentium. PLoS Pathogens, 17(4), e1009497.
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6

Murine AIEC Colonization Study

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AIEC, reference strain LF82 [16 (link)], was grown overnight in 200 mL of Luria-Bertani broth at 37°C without agitation. Twenty-four hours post-inoculation, bacterial suspensions, with OD620nm of 2.0, were placed in water bottles of three to four-week-old ASF C57BL/6 mice, which had been placed in isolated ventilated caging system (Isocage, Techniplast, Buguggiate VA, Italy) that prevents exogenous bacterial contamination [24 (link)]. Two weeks later, bacterial suspensions were removed and replaced with autoclaved drinking water, using a new sterile bottle. Fresh feces were collected every other week for downstream analysis. Eight-weeks post-inoculation, mice were fasted for 5 h at which time blood was collected by retro-bulbar intra-orbital capillary plexus. Hemolysis-free serum was generated by centrifugation of blood using serum separator tubes (Becton Dickinson, Franklin Lakes, NJ). Mice were then euthanized, and colon length, colon weight, spleen weight and adipose weight measured. Organs were collected for downstream analysis.
Chassaing B, & Gewirtz A.T. (2018). Mice harboring pathobiont-free microbiota do not develop intestinal inflammation that normally results from an innate immune deficiency. PLoS ONE, 13(4), e0195310.
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7

Establishment and Infection of ASF Mice

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C57BL/6 WT germ free mice were inoculated with ASF feces purchased from ASF Taconic, Inc.
(Hudson, NY), which contained the 8 ASF strains, to established Altered Schaedler Flora (ASF) mice as previously described [30] . Eight-week-old ASF male mice were fed with autoclaved chow and irradiated WSD for 1 week before infecting with 4×10 8 CFU/per mouse C. rodentium, and placed in isolated ventilated caging system (Isocage, Techniplast, Buguggiate VA, Italy) that prevents exogenous bacterial contamination. Fecal samples were collected at indicated days post infection, and the number of C. rodentium in feces was counted according to described above.
After 35 days, the mice were then removed from Isocages and transferred to ABSL2 unit, immediately orally administered 200 μl of the fecal suspension which generated from feces of conventional raised mice, and maintained in a sterile condition (autoclaved cage, food and water).
Gewirtz A.T., Zou J., Zhao X., & Noriega J. (2020). Western-style diet impedes colonization and clearance of gut bacterial pathogens.
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8

BALB/c Mice Infection Protocol

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Five-week-old female BALB/c mice were purchased from Japan SLC Inc., and housed in individually ventilated cages (Isocages; Techniplast) in enhanced BSL3 containment laboratories at the University of Tokyo. The mice were 6 weeks old at the time of infection. All experiments with mice were performed in accordance with the University of Tokyo’s Regulations for Animal Care and Use and approved by the Animal Experiment Committee of the Institute of Medical Science, the University of Tokyo.
Imai M., Watanabe T., Kiso M., Nakajima N., Yamayoshi S., Iwatsuki-Horimoto K., Hatta M., Yamada S., Ito M., Sakai-Tagawa Y., Shirakura M., Takashita E., Fujisaki S., McBride R., Thompson A.J., Takahashi K., Maemura T., Mitake H., Chiba S., Zhong G., Fan S., Oishi K., Yasuhara A., Takada K., Nakao T., Fukuyama S., Yamashita M., Lopes T.J., Neumann G., Odagiri T., Watanabe S., Shu Y., Paulson J.C., Hasegawa H, & Kawaoka Y. (2017). A highly pathogenic avian H7N9 influenza virus isolated from a human is lethal in some ferrets infected via respiratory droplets. Cell host & microbe, 22(5), 615-626.e8.
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9

Feeding Trials with C57Bl/6J Mice

Cited 2 times
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In the feeding trials, 54 female C57Bl/6J mice in maintenance metabolism were used (eight weeks old, bred by Envigo RMS B.V., Netherlands). The mice were housed in groups of 2–3 mice in isocages (Techniplast, Buguggiate, Italy) under specified-pathogen-free (SPF) conditions on silicate bedding (Tigerino Crystals, Matina GmbH, Munich, Germany). Two trials (Trials #1 and #2) were conducted consecutively and followed the procedure as described in previous publications [11 (link), 16 (link)] (study protocol approved by the Committee of the Faculty of Veterinary Medicine, LMU München, protocol code 169-03-05-2019). They did not involve any invasive procedures for the animals. The mice were sacrified by cervical dislocation after the experiment.
Böswald L.F., Wenderlein J., Siegert W., Straubinger R.K, & Kienzle E. (2023). True mineral digestibility in C57Bl/6J mice. PLOS ONE, 18(8), e0290145.
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10

Gnotobiotic Mouse Colonization Protocol

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Germ-free C57Bl/6 mice were colonized via oral gavage with 200μl of individual bacterial cultures or mixed bacterial consortia. Mock communities A and B consisted of the following taxa: Community A: Bacteroides spp; P. distasonis; Peptoniphilus spp; B. ovatus; Clostridiales UC/UC; Lachnospiraceae UC/UC; C. stercoris; B. uniformis and Parabacteroides spp.; and Community B: Streptococcus spp; C. perfringens; B. fragilis; Erisipelotrichaceae spp; C. aerofaciens; Bacteroides UC; B. producta; Allobaculum spp and Oscillospira spp. All strains were grown to roughly mid-log phase in GMM, mixed in equal ratios based on optical density, and then frozen at −80 °C in GMM containing 20% glycerol in rubber capped 2ml Wheaton vials until use. All gnotobiotic mice were maintained in Techniplast P Isocages and manipulated aseptically for the duration of the experiment. 16S rRNA gene sequencing of the V4 region to confirm colonization and microbial composition was performed essentially as described previously (Palm et al., 2014 (link)) except that data processing and analysis was done using QIIME2-DADA2 (Caporaso et al., 2010 (link)).
Chen H., Nwe P.K., Yang Y., Rosen C.E., Bielecka A.A., Kuchroo M., Cline G.W., Kruse A.C., Ring A.M., Crawford J.M, & Palm N.W. (2019). A forward chemical genetic screen reveals gut microbiota metabolites that modulate host physiology. Cell, 177(5), 1217-1231.e18.
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