Anti pdgfrα pe

Cells were analyzed using the LSR II (BD, Pharmingen) and sorted using FACSAria III (BD, Pharmingen), using FACSDiva software (BD, v8). Analysis was performed with FlowJo (v10). Samples were stained with the following antibodies at 1 ul antibody per 100ul cell suspension: anti-CD45 FITC, anti-CD31 FITC, anti-Ter119 FITC, anti-Sca1 Pacific Blue, anti-PDGFRα PE, anti-CD3 APC, anti-CD8 FITC, anti-CD45 Pacific Blue, anti-CD4 PE (all purchased from Biolegend). Tumors were enzymatically digested to single-cell suspension and filtered twice through 70 μm filters. Red blood cells were lysed with ACK solution (Gibco), washed twice with PBS, and then stained with the fluorophore-conjugated antibodies for 15 minutes at room temperature. The excess of unbound antibodies was washed out before acquisition in flow cytometry. Intracellular staining was performed to detect dsRNAs. Briefly, cells were fixed and permeabilized by using Cyto-Fast fix/perm buffers (Biolegend), following vendor’s protocol. After performing the staining for the surface markers, J2 antibody (Jena Bioscience, 2ug total) was added to the cells and incubated for 1 hour at room temperature, followed by incubation for 20 minutes with Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) secondary antibody (A32723, Invitrogen/Thermo Fisher).