Cy3 goat anti rabbit igg h l a0516

The following primary antibodies were used for western blotting: anti-ASC (ab180799), anti-caspase-1 (ab179515), anti-NLRP3 (ab263899), anti-gasdermin D (ab219800), anti-IL-1β (ab254360), anti-IL-18 (ab223293) (all purchased from Abcam, Cambridge, MA, USA), anti-F4/80 (28463–1-AP), anti-ZO-1 (21773–1-AP), anti-E-cadherin (20874–1-AP), and anti-AIM2 (66902–1-Ig) (all purchased from Proteintech). FITC goat anti-mouse IgG (H + L) and Cy3 goat anti-rabbit IgG (H + L) (A0516) were purchased from Beyotime Biotechnology (Haimen, China) and used for immunofluorescence analyses.

The primary antibodies used for western blot assay and immunohistochemical analysis were as follows: anti-GAPDH (10494-1-AP), anti-CD3 (17617-1-AP), anti-MBP (10458-1-AP), anti-IBA1 (10904-1-AP), anti-CD68 (28058-1-AP), and horseradish peroxidase (HRP)-conjugated secondary antibodies (SA00001-2) used for western blot analysis were purchased from Proteintech (Wuhan, China); anti-Catalase (CAT, 14097), anti-SOD2 (13141S) (Cell Signaling Technology, Danvers, MA, USA); anti-3-Nitrotyrosine (3-NT, AB5411, Millipore); anti-NAD(P)H dehydrogenase quinone 1 (NQO-1, ab34173), anti-NRF2 (ab31163), anti-TXNIP (ab232330), anti-NLRP3 (ab4207), anti-Caspase-1 (ab179515), anti-IL-18 (ab68435), anti-IL-1β (ab2105), and anti-ASC (ab127537) were purchased from Abcam (Cambridge, UK);. Cy3 goat anti-rabbit IgG (H+L) (A0516) and Alexa Fluor 488 goat anti-mouse IgG (H+L) (A0428) used for immunofluorescence analysis were purchased from Beyotime (Shanghai, China). The SPlink detection kits used for immunohistochemical analysis were purchased from ZSGB-BIO Technology (SP-9000) (Beijing, China). The antibodies used for flow cytometry analysis were as follows: PE-CYANINE anti-mouse IL-17 (25–7177–80), PE-CYANINE anti-mouse IFN-γ (25-7311-82), FITC anti-mouse CD4 (11-0041-82) (eBioscience, San Diego, CA, USA).